Human TLR4 Antibody

Catalog #: MAB11187 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11187-100
MAB11187-SP

Detection of Human TLR4 by Western Blot.

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All TLR4 Antibodies

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Human TLR4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TLR4 in direct ELISAs

Source

Monoclonal Mouse IgG_2A Clone # 1039412

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived human TLR4
Glu24-Lys631
Accession # O00206

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

RT4 human bladder carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human TLR4 by Western Blot. Western blot shows lysates of RT4 human bladder carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TLR4 Monoclonal Antibody (Catalog # MAB11187) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for TLR4 at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLR4

TLR4 is a 100 kDa type I transmembrane glycoprotein that belongs to the mammalian Toll‑Like Receptor family of pathogen pattern recognition molecules. The complex of TLR4 with MD‑2 functions as a critical receptor for bacterial endotoxin/lipopolysaccharide (LPS) (1-3). Mature human TLR4 consists of a 608 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 187 aa cytoplasmic domain. TLR4 contains 21 leucine rich repeats in its ECD and one cytoplasmic Toll/IL‑1 receptor (TIR) domain (4). The ECD of human TLR4 shares approximately 25% aa sequence identity with other human TLRs and 60%-74% aa sequence identity with bovine, equine, feline, mouse, rat, and porcine TLR4. On monocytes, macrophages, dendritic cells, and B cells, MD-2 expression is required for cell surface localization of TLR4 and for optimal LPS-induced TLR4 signaling (5-8). MD-2 also forms soluble disulfide‑linked homo‑oligomers which can interact with TLR4 (6). Through a domain separate from its TLR4-binding domain, MD-2 extracts LPS from circulating CD14-LPS complexes and carries the LPS into a ternary complex with TLR4 (9-11). The interaction of MD-2/LPS with TLR4 induces receptor oligomerization and the triggering of an inflammatory response (2, 12). Increased levels of plasma MD-2 in septic shock patients sensitizes MD-2 non-expressing epithelial cells to LPS and promotes widespread tissue inflammation (13).

References

Miyake, K. (2004) Semin. Immunol. 16:11.
Verstrepen, L. et al. (2008) Cell. Mol. Life Sci. 65:2964.
Erridge, C. (2010) J. Leukoc. Biol. 87:989.
Medzhitov, R. et al. (1997) Nature 388:394.
Shimazu, R. et al. (1999) J. Exp. Med. 189:1777.
Visintin, A. et al. (2001) Proc. Natl. Acad. Sci. 98:12156.
Akashi, S. et al. (2000) J. Immunol. 164:3471.
Nagai, Y. et al. (2002) Nat. Immunol. 3:667.
Re, F. and J.L. Strominger (2003) J. Immunol. 171:5272.
Kennedy, M.N. et al. (2004) J. Biol. Chem. 279:34698.
Gioannini, T.L. et al. (2004) Proc. Natl. Acad. Sci. 101:4186.
Saitoh, S. et al. (2004) J. Endotoxin Res. 10:257.
Pugin, J. et al. (2004) Blood 104:4071.

Long Name

Toll-like Receptor 4

Entrez Gene IDs

7099 (Human); 21898 (Mouse); 29260 (Rat)

Alternate Names

ARMD10; CD_antigen: CD284; CD284 antigen; CD284; EC 3.2.2.6; EC:3.2.2.6; homolog of Drosophila toll; hToll; TLR4; TLR-4; toll like receptor 4 protein; TOLL; toll-like receptor 4