Human TCF-3/E2A Antibody

Catalog # Availability Size / Price Qty
MAB44062
MAB44062-SP
Detection of Human TCF-3/E2A by Western Blot.
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Human TCF-3/E2A Antibody Summary

Species Reactivity
Human
Specificity
Detects human TCF-3/E2A in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) TCF-1 (aa 116‑233), rhTCF-1 (aa 217-358), rhTCF-1 alpha/LEF-1, rhTCF-2, rhTCF-4, rhTCF-12, rhTCF7L1 (aa 151-320), rhTCFL1 (aa 429-581), or rhTCF7L2 (aa 131-300) is observed.
Source
Monoclonal Rat IgG2A Clone # 401703
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E.coli-derived recombinant human TCF-3/E2A
Gln100-Glu500
Accession # NP_003191
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human TCF-3/E2A antibody by Western Blot. View Larger

Detection of Human TCF-3/E2A by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 2 µg/mL of Human TCF-3/E2A Monoclonal Antibody (Catalog # MAB44062) overnight at 4oC followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TCF-3/E2A at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TCF-3/E2A

TCF-3, also known as E2A and ITF-1, is a member of the E protein homeobox family. TCF-3 regulates checkpoints that allow B lineage commitment and B and T cell receptor gene rearrangement. Two isoforms named E12 and E47 are alternatively spliced within the DNA binding and HLH domain. The region of human TCF-3 used as the immunogen, which is identical for E12 and E47, shares 80% and 79% aa identity with mouse and rat TCF-3, respectively.

Long Name
Transcription Factor 3/E2-alpha
Entrez Gene IDs
6929 (Human); 21423 (Mouse); 312451 (Rat)
Alternate Names
bHLHb21; bHLHb21TCF-3; Class B basic helix-loop-helix protein 21; E2A immunoglobulin enhancer-binding factor E12/E47; E2A; E2AKappa-E2-binding factor; Immunoglobulin enhancer-binding factor E12/E47; Immunoglobulin transcription factor 1; ITF1; ITF1Transcription factor ITF-1; Kappa-E2-binding factor; MGC129647; MGC129648; Pan2; TCF-3; Tcfe2a; transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47); Transcription factor 3; transcription factor E2-alpha; VDIR; VDR interacting repressor

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