Human TAZ/WWTR1 Antibody Summary
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human TAZ/WWTR1 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TAZ/WWTR1 Monoclonal Antibody (Catalog # MAB7210) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TAZ/WWTR1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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TAZ/WWTR1 in BG01V Human Embryonic Stem Cells. TAZ/WWTR1 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human TAZ/WWTR1 Monoclonal Antibody (Catalog # MAB7210) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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TAZ/WWTR1 in A431 Human Cell Line. TAZ/WWTR1 was detected in immersion fixed A431 human epithelial carcinoma cell line using Mouse Anti-Human TAZ/WWTR1 Monoclonal Antibody (Catalog # MAB7210) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Detection of Human TAZ/WWTR1 by Western Blot Downregulation of CRB3 confers CSC traits on breast cancer cells through the TAZ/ beta -catenin cascade. (a) Western blot detecting the expressions of AMOT, TAZ and beta -catenin. (b) CoIP/western blot analysis showing endogenous AMOT bound to TAZ. (c) Fluorescence-activated cell sorting of CD44high/CD24low population. (d) Western blot of TAZ and stem cell markers. (e, g) Representative images and quantification of formed mammospheres. (f) Western blot of beta -catenin and stem cell markers. XAV939 was used to inhibit beta -catenin. (h) Flow cytometry analysis of the stem cell marker ALDH and the CD44high/CD24low population. All data are presented as mean±s.e.m. and statistical significance was calculated using a two-tailed t-test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28436991), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Preparation and Storage
Background: TAZ/WWTR1
TAZ (Transcriptional co-Activatorr with PDZ-binding motif; also WWTR1) is a 50-55 kDa protein that is related to YAP65 TEF-1 interacting protein. It is a widely expressed transcriptional coactivator, and should not be confused with tafazzin/Taz, an enzyme associated with lipid metabolism. TAZ influences the nuclear transport of SMAD-2, -3 and -4, and in the nucleus, TAZ is known to interact with transcription factors such as TFF1, Pax8, NKX2-1 and TEADS, serving as a scaffold for transcriptional activation complexes. Human TAZ is 400 amino acids (aa) in length. It contains one WW domain (aa 124-157) that binds to PPXY motifs, a coiled-coil region (aa 225-259), and a PDZ binding domain (aa 394-400). There are at least four utilized phosphorylation sites. When phosphorylated on Ser89, TAZ preferentially bind to 14-3-3 proteins, promoting its retention in the cytoplasm. Over aa 267-400, human TAZ shares 88% aa identity with mouse TAZ.
Product Datasheets
Citations for Human TAZ/WWTR1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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PYK2 is involved in premalignant acinar cell reprogramming and pancreatic ductal adenocarcinoma maintenance by phosphorylatingβ-cateninY654
Authors: C Gao, G Chen, DH Zhang, J Zhang, SF Kuan, W Hu, F Esni, X Gao, JL Guan, E Chu, J Hu
Cell Mol Gastroenterol Hepatol, 2019-07-19;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
CRB3 downregulation confers breast cancer stem cell traits through TAZ/?-catenin
Authors: P Li, Y Wang, X Mao, Y Jiang, J Liu, J Li, J Wang, R Wang, J She, J Zhang, J Yang, Y Liu, P Liu
Oncogenesis, 2017-04-24;6(4):e322.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot
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