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Human T-bet/TBX21 Fluorescein-conjugated Antibody

Catalog #: IC5385F
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
IC5385F
IC5385F-025
Detection of T‑bet/TBX21 in Jurkat Human Cell Line by Flow Cytometry.
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Citations (2)
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Human T-bet/TBX21 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human T-bet.
Source
Monoclonal Mouse IgG1 Clone # 525803
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human T-bet
Glu326-Asn535
Accession # Q9UL17
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of T-bet/TBX21 antibody in Jurkat Human Cell Line antibody by Flow Cytometry. View Larger

Detection of T‑bet/TBX21 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human T-bet/TBX21 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC5385F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: T-bet/TBX21

T-box expressed in T cells (T-bet), also known as T-box transcription factor TBX21, is a 62 kDa member of the T-box family of transcription factors and the Tbr1 subfamily. Human T-bet is 535 amino acids in length and contains a T-box DNA binding domain (aa 136-327). Human T-bet shares 88% aa sequence identity with mouse T-bet. T-bet is a nuclear protein highly apparent in Th1-cells. Northern blot analysis revealed that it is also expressed in lung, thymus and spleen. Functionally, T-bet controls the expression of the Th1 cytokine, IFN gamma, and initiates Th1 lineage development from naïve Th precursor cells by both activating Th1 genetic programs and by repressing the opposing Th2 programs.

Long Name
T-box transcription factor TBX21
Entrez Gene IDs
30009 (Human); 57765 (Mouse); 303496 (Rat)
Alternate Names
Tbet; T-bet; TBETT-PET; TBLYM; TBLYMT-cell-specific T-box transcription factor T-bet; T-box 21; T-box expressed in T cells; T-box protein 21; T-box transcription factor TBX21; TBX21; T-PET; Transcription factor TBLYM

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Citations for Human T-bet/TBX21 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Combined in vitro IL-12 and IL-15 stimulation promotes cellular immune response in dogs with visceral leishmaniasis
    Authors: SF Costa, VO Gomes, MO Dos Santos, LM Melo, GL Venturin, JP Bragato, GT Rebech, C de Oliveir, BM Nascimento, G Gileno de, VM Felix de L
    PLoS Negl Trop Dis, 2020-01-21;14(1):e0008021.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Induction of miR 21 impairs the anti-Leishmania response through inhibition of IL-12 in canine splenic leukocytes
    Authors: LM Melo, JP Bragato, GL Venturin, GT Rebech, SF Costa, LE Garcia, FL Lopes, FR Eugênio, PS Patto Dos, VMF de Lima
    PLoS ONE, 2019-12-11;14(12):e0226192.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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