Human sTransferrin Receptor/sTfR Quantikine ELISA, RUO New

Catalog #: DTFRU0 Datasheet / COA / SDS

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QC302: Quantikine Immunoassay Control Set 1143 for Human sTfR RUO

Human sTfR Quantikine ELISA Standard Curve

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human sTransferrin Receptor/sTfR Quantikine ELISA, RUO Summary

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Serum (25 uL), EDTA Plasma (50 uL), Heparin Plasma (25 uL)

Sensitivity

0.064 nmol/L

Assay Range

1.3 - 40 nmol/L (Serum, EDTA Plasma, Heparin Plasma)

Specificity

This assay recognizes natural human sTfR.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human sTfR Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human sTfR in serum and plasma. Results obtained using natural human sTfR showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human sTfR.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (nmol/L)	4.82	18.4	28.8	5.02	19.8	29.5
Standard Deviation	0.115	0.375	0.791	0.202	1.31	2.56
CV%	2.4	2	2.7	4	6.6	8.7

Recovery

The recovery of human sTfR spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	107	98-114
Heparin Plasma (n=4)	107	96-119
Serum (n=4)	109	94-116

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of human sTfR in various matrices were diluted with the calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data

N/A

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Human sTfR Quantikine ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TfR (Transferrin R)

Transferrin receptors are transmembrane, homodimeric proteins that play a role in iron transport, uptake, and storage. TfR is an essential receptor, found on almost all cell types, regulated by intracellular iron concentration, and has a high affiinity for transferrin. Soluble TfR (sTfR) arises from the proteolysis of TfR into monomers that can be measured in plasma and serum. TfR2 has limited tissue distribution (hepatocytes and enterocytes), is regulated by the cell cycle, and binds transferrin poorly.

Long Name:

Transferrin Receptor

Entrez Gene IDs:

7037 (Human); 22042 (Mouse); 64678 (Rat)

Alternate Names:

CD71 antigen; CD71; CD71TRFR; p90; T9; TfR (Transferrin R); TfR (TransferrinR); TfR; TFR1; TFRC; TR; transferrin receptor (p90, CD71); transferrin receptor protein 1; TRFR