Human STEAP1 APC-conjugated Antibody

Name: Human STEAP1 APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB55871A
Rating: 1 (1 reviews)

Catalog #: FAB55871A 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB55871A-100

Detection of STEAP1 in HEK293 cells by Flow Cytometry.

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Human STEAP1 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human STEAP1 in direct ELISA.

Source

Monoclonal Rabbit IgG Clone # 2959F

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human STEAP1
Met1-Trp71
Accession # Q9UHE8

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

see below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of STEAP1 in HEK293 cells by Flow Cytometry. HEK293 cells transfected with Human STEAP-1 and eGFP (A) vs irrelevant cells (B) were stained with Rabbit Anti-Human STEAP1 APC-conjugated MonoclonalAntibody (Catalog # FAB55871A). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of STEAP1 in LnCaP cells by Flow Cytometry LnCaP cells were stained with Rabbit Anti-Human STEAP1 APC‑conjugated Monoclonal Antibody (Catalog # FAB55871A, filled histogram) or isotype control antibody (Catalog # IC1051A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with saponin. View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: STEAP1

STEAP1 (six-transmembrane epithelial antigen of the prostate-1) is a 40 kDa protein (predicted) of the STEAP family of metalloreductases. It is expressed mainly at cell‑cell junctions between prostate secretory epithelium, and up‑regulated in prostate and some bladder, colon and ovarian cancers and Ewing’s sarcomas. Human STEAP1 is 339 amino acids (aa) in length. It contains a ferric oxidoreductase domain (aa 119‑265) that includes transmembrane sequences (# 2, 3, 4, and part of 5, out of 6 sequences). Two splice forms diverge at aa 255, terminating after aa 58 or 259. Over aa 1‑71, human and mouse STEAP1 share 68% aa identity.

Long Name

Six Transmembrane Epithelial Antigen of the Prostate 1

Entrez Gene IDs

26872 (Human); 70358 (Mouse); 297738 (Rat)

Alternate Names

EC 1.16.1.-; PRSS24; PRSS24MGC19484; six transmembrane epithelial antigen of the prostate 1; six transmembrane epithelial antigen of the prostate; Six-transmembrane epithelial antigen of prostate 1; STEAP1; STEAPmetalloreductase STEAP1