Human Siglec-7/CD328 Antibody

Catalog #: MAB1138
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1138
MAB1138-SP

Product Details

Citations (4)

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Siglec-7/CD328 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Siglec‑7/CD328 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Siglec-2, -3, -5, or -9 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 194212

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Siglec‑7/CD328
Gln19-Gly357 (predicted)
Accession # Q9Y286

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Siglec‑7 Fc Chimera (Catalog # 1138-SL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-7/CD328

Siglecs (1) (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (1, 2). Eleven human Siglecs have been cloned and characterized. They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglecs 5 to 11 (1‑4). To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Siglecs 5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. They are collectively referred to as CD33-related Siglecs. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system (2, 3). This fact, together with the presence of two conserved immunoreceptor tyrosine-based inhibition motifs (ITIMs) in their cytoplasmic tails, suggests that CD33-related Siglecs are involved in the regulation of cellular activation within the immune system.

The cDNA of human Siglec-7, also known as adhesion inhibitory receptor module-1 (AIRM-1) and designated CD328, encodes a 467 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, two Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail (5). Siglec-7 exists as a monomer on the cell surface and is expressed on natural killer cells, CD8⁺ T cells and monocytes (3, 5). It binds equally well to both alpha 2,3- and alpha 2,6-linked sialic acid (5). The gene encoding Siglec-7 was mapped to chromosome 19q13.3.

References

Crocker, P.R. et al. (1998) Glycobiology 8:v.
Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337.
Crocker, P.R. and A. Varki (2001) Immunology 103:137.
Angata, T. et al. (2002) J. Biol. Chem. 277:24466.
Nicoll, G. et al. (1999) J. Biol. Chem. 274:34089.

Long Name

Sialic Acid Binding Ig-like Lectin 7

Entrez Gene IDs

27036 (Human)

Alternate Names

Adhesion inhibitory receptor molecule 1; adhesion inhibitory receptor molecule 1, siglec-7; AIRM1; AIRM-1; AIRM1QA79 membrane protein; CD328 antigen; CD328; CDw328; D-siglec; p75; p75/AIRM1; QA79; sialic acid binding Ig-like lectin 7; sialic acid binding immunoglobulin-like lectin 7; sialic acid-binding Ig-like lectin 7; Siglec7; Siglec-7

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Siglec-7/CD328 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
Filter your results:

Filter by:

Decidual glycodelin-A polarizes human monocytes towards a decidual macrophage-like phenotype via siglec-7
Authors: M Vijayan, CL Lee, VHH Wong, X Wang, K Bai, J Wu, H Koistinen, M Seppälä, KF Lee, WSB Yeung, EHY Ng, PCN Chiu
J. Cell. Sci., 2020-07-23;0(0):.
Species: Human
Sample Types: Protein
Applications: Western Blot
Glycocalyx engineering reveals a Siglec-based mechanism for NK cell immunoevasion.
Authors: Hudak, Jason E, Canham, Stephen, Bertozzi, Carolyn
Nat Chem Biol, 2013-11-24;10(1):69-75.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Siglec-7 tetramers characterize B-cell subpopulations and leukemic blasts.
Eur J Immunol, 2012-07-09;42(8):2176-86.
Species: Human
Sample Types: Recombinant Protein
Applications: Western Blot
CD molecules 2006--human cell differentiation molecules.
Authors: Zola H, Swart B, Banham A, Barry S, Beare A, Bensussan A, Boumsell L, D Buckley C, Buhring HJ, Clark G, Engel P, Fox D, Jin BQ, Macardle PJ, Malavasi F, Mason D, Stockinger H, Yang X
J. Immunol. Methods, 2006-12-04;319(1):1-5.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry