Home / Serpin A8/Angiotensinogen / Human Serpin A8/Angiotensinogen Antibody

Human Serpin A8/Angiotensinogen Antibody

Catalog #: AF3156
12 Citations Datasheet / COA / SDS
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AF3156
AF3156-SP
Detection of Human Serpin A8/Angiotensinogen by Western Blot.
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Citations (12)
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Human Serpin A8/Angiotensinogen Antibody Summary

Species Reactivity
Human
Specificity
Detects human Serpin A8/Angiotensinogen in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Serpin A8/Angiotensinogen is observed, and less than 5% cross-reactivity with recombinant human Serpin A1, A4, A5, F2, G1, I1, and I2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Serpin A8/Angiotensinogen
Asp34-Ala485
Accession # ABM85122
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
2-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Serpin A8/Angiotensinogen antibody by Western Blot. View Larger

Detection of Human Serpin A8/Angiotensinogen by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Serpin A8/Angiotensinogen Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3156) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Serpin A8/Angiotensinogen at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Serpin A8/Angiotensinogen antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

Serpin A8/Angiotensinogen in Human Brain. Serpin A8/Angiotensinogen was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 1.7 µg/mL Goat Anti-Human Serpin A8/Angiotensinogen Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3156) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human Serpin A8/Angiotensinogen antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human Serpin A8/Angiotensinogen by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Serpin A8/Angiotensinogen at approximately 64 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Serpin A8/Angiotensinogen Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3156) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin A8/Angiotensinogen

Serpin A8 (serine proteinase inhibitor-clade A #8; also angiotensinogen/ANGT) is a secreted, 52-62 kDa glycoprotein member of the clade F-subfamily, serpin superfamily of protease inhibitors.  It is expressed by neurons and hepatocytes, and undergoes extracellular cleavage by renin to create a ten amino acid (aa) peptide termed Ang/angiotensin I.  This inactive peptide is further cleaved by ACE on the endothelial cell membrane to create bioactive Ang II.  Ang II induces vascoconstriction and aldosterone release.  Mature human angiotensinogen is 452 aa in length (aa 34-485).  It contains Ang I (aa 34-43) that is cleaved to create Ang II (aa 34-41).  Angiotensinogen may circulate in a 200 kDa complex with major basic protein (MBP), or as part of a larger 300 kDa complex with MBP and complement C3dg.  Over aa 34-485, human serpin A8 shares 61% aa identity with mouse serpin A8.        

Entrez Gene IDs
183 (Human); 11606 (Mouse); 24179 (Rat)
Alternate Names
AGT; alpha-1 antiproteinase, antitrypsin; Angiotensin II; angiotensinogen (serpin peptidase inhibitor, clade A, member 8); Angiotensinogen; ANHU; Aogen; FLJ92595; FLJ97926; PAT; pre-angiotensinogen; serine (or cysteine) proteinase inhibitor; Serpin A8; SERPINA8angiotensin I

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Citations for Human Serpin A8/Angiotensinogen Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. miR-6869-5p Transported by Plasma Extracellular Vesicles Mediates Renal Tubule Injury and Renin-Angiotensin System Activation in Obesity
    Authors: Liu HH, Li XQ, Liu JF et al.
    Frontiers in Medicine
  2. The muscular expression of RAS in patients with achalasia
    Authors: A Casselbrant, S Kostic, H Lönroth
    Journal of the Renin-Angiotensin-Aldosterone System
  3. Expression of classical components of the renin-angiotensin system in the human eye
    Authors: Andrew J R White, Sarat C Cheruvu, Maria Sarris, Surabhi S Liyanage, Eugenie Lumbers, Jeanie Chui et al.
    Journal of the Renin-Angiotensin-Aldosterone System
  4. Expression of angiotensinogen and receptors for angiotensin and prorenin in the monkey and human substantia nigra: an intracellular renin–angiotensin system in the nigra
    Authors: Pablo Garrido-Gil, Rita Valenzuela, Begoña Villar-Cheda, Jose L. Lanciego, Jose L. Labandeira-Garcia
    Brain Structure and Function
  5. Molecular characterization of renin-angiotensin system components in human intrauterine tissues and fetal membranes from vaginal delivery and cesarean section
    Authors: F.Z. Marques, K.G. Pringle, A. Conquest, J.J. Hirst, M.A. Markus, M. Sarris et al.
    Placenta
  6. The expression of renin-angiotensin system components in the human gastric mucosa
    Authors: Peter Hallersund, Anders Elfvin, Herbert F Helander, Lars Fändriks
    Journal of the Renin-Angiotensin-Aldosterone System
  7. CSF protein ratios with enhanced potential to reflect Alzheimer's disease pathology and neurodegeneration
    Authors: Mravinacová, S;Alanko, V;Bergström, S;Bridel, C;Pijnenburg, Y;Hagman, G;Kivipelto, M;Teunissen, C;Nilsson, P;Matton, A;Månberg, A;
    Molecular neurodegeneration
    Species: Human
    Sample Types: Cerebrospinal Fluid
    Applications: ELISA Capture
  8. miR-6869-5p Transported by Plasma Extracellular Vesicles Mediates Renal Tubule Injury and Renin-Angiotensin System Activation in Obesity
    Authors: Liu HH, Li XQ, Liu JF et al.
    Frontiers in Medicine
  9. Expression of renin angiotensin system (RAS) components in endometrial cancer
    Authors: Sarah J Delforce
    Endocr Connect, 2016-12-12;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. Hepatocarcinogenesis driven by GSNOR deficiency is prevented by iNOS inhibition.
    Authors: Tang C, Wei W, Hanes M, Liu L
    Cancer Res, 2013-02-25;73(9):2897-904.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  11. The inflammatory response in the MPTP model of Parkinson's disease is mediated by brain angiotensin: relevance to progression of the disease.
    Authors: Joglar B, Rodriguez-Pallares J, Rodriguez-Perez AI, Rey P, Guerra MJ, Labandeira-Garcia JL
    J. Neurochem., 2009-02-20;109(2):656-69.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  12. The expression and localization of the human placental prorenin/renin-angiotensin system throughout pregnancy: Roles in trophoblast invasion and angiogenesis?
    Authors: K.G. Pringle, M.A. Tadros, R.J. Callister, E.R. Lumbers
    Placenta

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