Human S100B Antibody

Catalog #: MAB18202 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB18202-100
MAB18202-SP

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Human S100B Antibody Summary

Species Reactivity

Human

Specificity

Detects human S100B in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 721713

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human S100B
Met1-Glu92
Accession # P04271

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human S100B Monoclonal Antibody (Catalog # MAB18201).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human S100B DuoSet ELISA Kit (Catalog # DY1820-05) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human S100B ELISA Standard Curve. Recombinant Human S100B protein was serially diluted 2-fold and captured by Mouse Anti-Human S100B Monoclonal Antibody (Catalog # MAB18201) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human S100B Monoclonal Antibody (Catalog # MAB18202) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S100B

S100B belongs to the S100 subgroup of the EF-hand family of Ca²⁺ binding proteins. It is a homodimer that is expressed primarily in the brain by astrocytes, oligodendrocytes and Schwann cells. S100B has multiple intracellular functions, but can also be secreted from cells to exert extracellular functions. Some of the extracellular functions of S100B may be mediated by RAGE (receptor for advanced glycation end products). Blood levels of S100B can be used to monitor the extent of brain injury and malignant melanoma.

Long Name

S100 Calcium Binding Protein B

Entrez Gene IDs

6285 (Human)

Alternate Names

beta (neural); NEF; S100 beta; S100 calcium binding protein B; S100 calcium-binding protein B; S100 calcium-binding protein, beta (neural); S-100 calcium-binding protein, beta chain, 10protein S100-B; S-100 protein beta chain; S-100 protein subunit beta; S100; S100B; S100beta