Human Ribosomal Protein S6/RPS6 Biotinylated Antibody

Catalog #: BAM5436 Datasheet / COA / SDS

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BAM5436

Ribosomal Protein S6/RPS6 in HeLa Human Cell Line.

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Human Ribosomal Protein S6/RPS6 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Conjugated Ribosomal Protein S6/RPS6 antibodies are ideal for immunocytochemistry colocalization studies in ribosomes. Detects human Ribosomal Protein S6/RPS6 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 522731

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Ribosomal Protein S6/RPS6
Met1-Lys249
Accession # P62753

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Ribosomal Protein S6/RPS6 in HeLa Human Cell Line. Ribosomal Protein S6/RPS6 was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Ribosomal Protein S6/RPS6 Biotinylated Monoclonal Antibody (Catalog # BAM5436) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (orange; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to ribosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ribosomal Protein S6/RPS6

40S Ribosomal Protein S6 (RPS6) is the major substrate of protein kinases, particularly p70 S6 kinase, in eukaryotic ribosomes. RPS6 phosphorylation at S235, S236, S240, and S244 upregulates the translation of mRNAs containing an oligopyrimidine tract at their transcriptional start sites. This phosphorylation is stimulated by growth factors, tumor promoting agents, and other mitogens. RPS6 is dephosphorylated during growth arrest.

Entrez Gene IDs

6194 (Human); 20104 (Mouse); 29304 (Rat)

Alternate Names

Phosphoprotein NP33, 40S ribosomal protein S6; pS6; Ribosomal Protein S6; RPS6; S6