Human/Rat Lyn Antibody

Catalog #: MAB3206 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3206
MAB3206-SP

Detection of Human/Rat Lyn by Western Blot.

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Human/Rat Lyn Antibody Summary

Species Reactivity

Human, Rat

Specificity

Detects human and rat Lyn.

Source

Monoclonal Mouse IgG_2B Clone # 338716

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Lyn
Met1-Ile67
Accession # P07948

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Simple Western

5 µg/mL

Ramos human Burkitt's lymphoma cell line

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Rat Lyn by Western Blot. Western blot shows lysates of Ramos human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Rat Lyn Monoclonal Antibody (Catalog # MAB3206) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Lyn at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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Lyn in Raji Human Cell Line. Lyn was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Mouse Anti-Human/Rat Lyn Monoclonal Antibody (Catalog # MAB3206) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Simple Western

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Detection of Human Lyn by Simple Western^TM. Simple Western lane view shows lysates of Ramos human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Lyn at approximately 58 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human/Rat Lyn Monoclonal Antibody (Catalog # MAB3206). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lyn

The nonreceptor tyrosine kinase Lyn (lck/yes-related novel tyrosine kinase) is widely expressed, and the predominant Src family member present in B cells. Activation of Lyn occurs upon its association with cell surface receptors such as BCR and CD40. Analyses from both loss- and gain-of-function mutant mice have revealed that Lyn is an essential regulator of B cell activation, maturation, and tolerance.

Long Name

V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog

Entrez Gene IDs

4067 (Human); 17096 (Mouse); 81515 (Rat)

Alternate Names

EC 2.7.10; EC 2.7.10.2; FLJ26625; Hck-2; JTK8; Lyn; tyrosine-protein kinase Lyn; v-yes-1 Yamaguchi sarcoma viral related oncogene homolog; v-yes-1; Yamaguchi sarcoma viral (v-yes-1) related oncogene homolog