Home / Protocadherin-8 / Human Protocadherin-8 Antibody

Human Protocadherin-8 Antibody

Catalog #: MAB5434
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB5434
MAB5434-SP
Protocadherin‑8 in MCF‑7 Human Cell Line.
1 Image
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Protocadherin-8 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Protocadherin-8 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Protocadherin-1, -10, or -15 is observed.
Source
Monoclonal Mouse IgG2B Clone # 562615
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Protocadherin-8
Lys30-Thr479
Accession # O95206
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Protocadherin-8 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC). View Larger

Protocadherin‑8 in MCF‑7 Human Cell Line. Protocadherin-8 was detected in immersion fixed MCF-7 human breast cancer cell line using Human Protocadherin-8 Monoclonal Antibody (Catalog # MAB5434) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Protocadherin-8

Protocadherin 8 (PCDH-8) is a 160 kDa group F member of the protocadherin family of molecules.  It is expressed in breast epithelium, the synaptic membranes of neurons, and presumably forms Ca++-dependent homophilic complexes.  In breast, it is believed to contribute to a stable epithelial architecture, while in brain, it impacts LTP.  Mature human PCDH-8 is a type I transmembrane protein that is 1041 amino acids (aa) in length.  It contains a 720aa extracellular domain (ECD) (aa 30-749) plus a 300 aa cytoplasmic region.  There are six cadherin domains in the ECD (aa 30-723).  Multiple splice variants exist.  Two show deletions of aa 134-607 and 781-877, respectively, while a third shows a six aa substitution for aa 365-527.  Over aa 30-689, human PCDH-8 shares 93% aa identity with mouse PCDH-8.

Entrez Gene IDs
5100 (Human); 18530 (Mouse); 64865 (Rat)
Alternate Names
Arcadlin; PAPC; PCDH8; protocadherin 8; Protocadherin8; Protocadherin-8

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citation for Human Protocadherin-8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Transcription factor Oct1 is a somatic and cancer stem cell determinant.
    Authors: Maddox, Jessica, Shakya, Arvind, South, Samuel, Shelton, Dawne, Andersen, Jared N, Chidester, Stephani, Kang, Jinsuk, Gligorich, Keith M, Jones, David A, Spangrude, Gerald J, Welm, Bryan E, Tantin, Dean
    PLoS Genet, 2012-11-08;8(11):e1003048.

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human Protocadherin-8 Antibody

There are currently no reviews for this product. Be the first to review Human Protocadherin-8 Antibody and earn rewards!

Have you used Human Protocadherin-8 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review