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Human Pro-MMP-10 Quantikine ELISA Kit

Catalog #: DM1000
14 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DM1000
Control Products Available
QC131: Quantikine Immunoassay Control Set 1180 for Human Pro-MMP-10
Human Pro-MMP-10 ELISA Standard Curve
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Product Details
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Citations (14)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human Pro-MMP-10 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (25 uL), Heparin Plasma (25 uL)
Sensitivity
15.1 pg/mL
Assay Range
78.1 - 5,000 pg/mL (Cell Culture Supernates, Serum, Heparin Plasma)
Specificity
Natural and recombinant human pro-MMP-10
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Pro-MMP-10 Immunoassay is a 4.5 hour solid phase immunoassay designed to measure pro-MMP-10 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human pro-MMP-10 and antibodies raised against the recombinant protein. This kit will not detect human active MMP-10 existing in either free or TIMP-bound form. Natural human pro-MMP-10 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human pro-MMP-10.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 515 2497 3442 515 2542 3592
Standard Deviation 22.9 115 137 30.4 119 153
CV% 4.4 4.6 4 5.9 4.7 4.3

Recovery

The recovery of pro-MMP-10 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 99 88-109

Linearity

To assess the linearity of the assay, samples containing or spiked with high concentrations of pro-MMP-10 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Pro-MMP-10 ELISA Linearity

Scientific Data

N/A Human Pro-MMP-10 ELISA Standard Curve View Larger

Human Pro-MMP-10 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MMP-10

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

Long Name:
Matrix Metalloproteinase 10
Entrez Gene IDs:
4319 (Human)
Alternate Names:
EC 3.4.24; EC 3.4.24.22; matrix metallopeptidase 10 (stromelysin 2); matrix metalloprotease 10; matrix metalloproteinase 10 (stromelysin 2); Matrix metalloproteinase-10; MMP10; MMP-10; SL-2; STMY2stromelysin-2; Stromelysin 2; transin 2; transin-2
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human Pro-MMP-10 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Association between Circulating Levels of 25-Hydroxyvitamin D3 and Matrix Metalloproteinase-10 (MMP-10) in Patients with Type 2 Diabetes
    Authors: D Abasheva, MM Dolcet-Neg, MA Fernández-, JM Mora-Gutié, J Orbe, FJ Escalada, N Garcia-Fer
    Nutrients, 2022-08-24;14(17):.
    Species: Human
    Sample Types: Serum
  2. Matrix Metalloproteinase 10 Contributes to Choroidal Neovascularisation
    Authors: J González-Z, M Hernandez, S Recalde, J Bezunartea, A Montoliu, V Bilbao-Mal, J Orbe, JA Rodríguez, S Llorente-G, P Fernández-, A García-Lay
    Biomedicines, 2022-06-30;10(7):.
    Species: Human
    Sample Types: Plasma
  3. MMP-10 is Increased in Early Stage Diabetic Kidney Disease and can be Reduced by Renin-Angiotensin System Blockade
    Authors: JM Mora-Gutié, JA Rodríguez, MA Fernández-, J Orbe, FJ Escalada, MJ Soler, MF Slon Roble, M Riera, JA Páramo, N Garcia-Fer
    Sci Rep, 2020-01-08;10(1):26.
    Species: Human
    Sample Types: Serum
  4. A multiplex immunoassay for the non-invasive detection of bladder cancer.
    Authors: Shimizu Y, Furuya H, Bryant Greenwood P, Chan O, Dai Y, Thornquist M, Goodison S, Rosser C
    J Transl Med, 2016-01-30;14(1):31.
    Species: Human
    Sample Types: Urine
  5. Suppression of Aurora-A-FLJ10540 signaling axis prohibits the malignant state of head and neck cancer.
    Authors: Chen, Chang-Ha, Chang, Alice Y, Li, Shau-Hsu, Tsai, Hsin-Tin, Shiu, Li-Yen, Su, Li-Jen, Wang, Wen-Lung, Chiu, Tai-Jen, Luo, Sheng-De, Huang, Tai-Lin, Chien, Chih-Yen
    Mol Cancer, 2015-04-12;14(0):83.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. MMP-1 and Pro-MMP-10 as potential urinary pharmacodynamic biomarkers of FGFR3-targeted therapy in patients with bladder cancer.
    Authors: Du X, Lin B, Wang Q, Li H, Ingalla E, Tien J, Rooney I, Ashkenazi A, Penuel E, Qing J
    Clin Cancer Res, 2014-10-17;20(24):6324-35.
    Species: Human
    Sample Types: Serum
  7. Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation.
    Authors: Reikvam H, Hatfield KJ, Oyan AM, Kalland KH, Kittang AO, Bruserud O
    Eur. J. Haematol., 2009-11-17;84(3):239-51.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Matrix metalloproteinase-9, -10, and tissue inhibitor of matrix metalloproteinases-1 blood levels as biomarkers of severity and mortality in sepsis.
    Authors: Lorente L, Martin MM, Labarta L, Diaz C, Sole-Violan J, Blanquer J, Orbe J, Rodriguez JA, Jimenez A, Borreguero-Leon JM, Belmonte F, Medina JC, Lliminana MC, Ferrer-Aguero JM, Ferreres J, Mora ML, Lubillo S, Sanchez M, Barrios Y, Sierra A, Paramo JA
    Crit Care, 2009-10-02;13(5):R158.
    Species: Human
    Sample Types: Serum
  9. Advanced glycation end products decrease mesangial cell MMP-7: a role in matrix accumulation in diabetic nephropathy?
    Authors: McLennan SV, Kelly DJ, Schache M, Waltham M, Dy V, Langham RG, Yue DK, Gilbert RE
    Kidney Int., 2007-06-06;72(4):481-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Matrix metalloproteinase 10 promotion of collagenolysis via procollagenase activation: implications for cartilage degradation in arthritis.
    Authors: Barksby HE, Milner JM, Patterson AM, Peake NJ, Hui W, Robson T, Lakey R, Middleton J, Cawston TE, Richards CD, Rowan AD
    Arthritis Rheum., 2006-10-01;54(10):3244-53.
    Species: Human
    Sample Types: Synovial Fluid
  11. C-reactive protein induces matrix metalloproteinase-1 and -10 in human endothelial cells: implications for clinical and subclinical atherosclerosis.
    Authors: Montero I, Orbe J, Varo N, Beloqui O, Monreal JI, Rodriguez JA, Diez J, Libby P, Paramo JA
    J. Am. Coll. Cardiol., 2006-03-20;47(7):1369-78.
    Species: Human
    Sample Types: Whole Cells
  12. Induction of host matrix metalloproteinases by Borrelia burgdorferi differs in human and murine lyme arthritis.
    Authors: Behera AK, Hildebrand E, Scagliotti J, Steere AC, Hu LT
    Infect. Immun., 2005-01-01;73(1):126-34.
    Species: Human
    Sample Types: Synovial Fluid
  13. CXCL12-CXCR4 interactions modulate prostate cancer cell migration, metalloproteinase expression and invasion.
    Authors: Singh S, Singh UP, Grizzle WE, Lillard JW
    Lab. Invest., 2004-12-01;84(12):1666-76.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Induction of MMP-10 and MMP-1 in a squamous cell carcinoma cell line by ultraviolet radiation.
    Authors: Ramos MC, Steinbrenner H, Stuhlmann D, Sies H, Brenneisen P
    Biol. Chem., 2004-01-01;385(1):75-86.
    Species: Human
    Sample Types: Cell Culture Supernates

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