Human/Primate EGF Biotinylated Antibody Summary
Applications
Human/Primate EGF Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EGF
Epidermal growth factor (EGF) is the founding member of the EGF family that also includes TGF-alpha, amphiregulin (AR), betacellulin (BTC), epiregulin (EPR), heparin-binding EGF-like growth factor (HB-EGF), epigen, and the neuregulins (NRG)-1 through -6 (1). Members of the EGF family share a structural motif, the EGF-like domain, which is characterized by three intramolecular disulfide bonds that are formed by six similarly spaced conserved cysteine residues (2). All EGF family members are synthesized as type I transmembrane precursor proteins that may contain several EGF domains in the extracellular region. The mature proteins are released from the cell surface by regulated proteolysis (1). The 1207 amino acid (aa) human EGF precursor contains nine EGF domains and nine LDLR class B repeats. The mature protein consists of 53 aa and is generated by proteolytic excision of the EGF domain proximal to the transmembrane region (3). Mature human EGF shares 70% aa sequence identity with mature mouse and rat EGF. EGF is present in various body fluids, including blood, milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid (4). Four ErbB (HER) family receptor tyrosine kinases including EGFR/ErbB1, ErbB2, ErbB3 and ErbB4, mediate responses to EGF family members (5). These receptors undergo a complex pattern of ligand induced homo- or hetero-dimerization to transduce EGF family signals (6, 7). EGF binds ErbB1 and depending on the context, induces the formation of homodimers or heterodimers containing ErbB2. Dimerization results in autophosphorylation of the receptor at specific tyrosine residues to create docking sites for a variety of signaling molecules (5, 8). Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture.
- Harris, R.C. et al. (2003) Exp. Cell Res. 284:2.
- Carpenter, G. and Cohen, S. (1990) J. Biol. Chem. 265:7709.
- Bell, G.I. et al. (1986) Nucl. Acids Res. 14:8427.
- Carpenter, G. and Zendegui, J.G. (1986) Exp. Cell Res. 164:1.
- Jorissen, R.N. et al. (2003) Exp. Cell Res. 284:31.
- Gamett, D.C. et al. (1997) J. Biol. Chem. 272:12052.
- Qian, X. et al. (1994) Proc. Natl. Acad. Sci. 91:1500.
- Qian, X. et al. (1999) J. Biol. Chem. 274:574.
Product Datasheets
Citations for Human/Primate EGF Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Generation of hypoxia-sensing chimeric antigen receptor T cells
Authors: Paris Kosti, Karen I. Larios-Martinez, John Maher, James N. Arnold
STAR Protocols
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Intratumoral pan-ErbB targeted CAR-T for head and neck squamous cell carcinoma: interim analysis of the T4 immunotherapy study
Authors: Sophie Papa, Antonella Adami, Michael Metoudi, Richard Beatson, Molly Sarah George, Daniela Achkova et al.
Journal for ImmunoTherapy of Cancer
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Synergism between Hedgehog-GLI and EGFR Signaling in Hedgehog-Responsive Human Medulloblastoma Cells Induces Downregulation of Canonical Hedgehog-Target Genes and Stabilized Expression of GLI1
Authors: Frank Götschel, Daniela Berg, Wolfgang Gruber, Christian Bender, Markus Eberl, Myriam Friedel et al.
PLoS ONE
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Generation of human parallel chimeric antigen receptor (pCAR) T cells to achieve synergistic T cell co-stimulation
Authors: Daniel Larcombe-Young, Lynsey Whilding, David Marc Davies, Benjamin Draper, Natasha Bechman, John Maher
STAR Protocols
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Generation and application of TGF beta -educated human V gamma 9Vδ2 T cells
Authors: Ana C. Parente-Pereira, Richard E. Beatson, David M. Davies, Caroline Hull, Lynsey M. Whilding, Joanna C. Porter et al.
STAR Protocols
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Hypoxia-sensing CAR T cells provide safety and efficacy in treating solid tumors
Authors: Kosti P, Opzoomer J, Larios-Martinez K et al.
Cell Reports Medicine
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Development and Validation of a Good Manufacturing Process for IL-4-Driven Expansion of Chimeric Cytokine Receptor-Expressing CAR T-Cells
Authors: MCI van Schalk, SJC van der St, L Bosshard-C, H Graves, S Papa, AC Parente-Pe, F Farzaneh, CD Fisher, A Hope, A Adami, J Maher
Cells, 2021-07-15;10(7):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry (CAR-T) -
Accumulation of hEGF and hEGF-fusion proteins in chloroplast-transformed tobacco plants is higher in the dark than in the light.
Authors: Wirth S, Segretin ME, Mentaberry A, Bravo-Almonacid F
J. Biotechnol., 2006-04-03;125(2):159-72.
Species: Human
Sample Types: Tissue Homogenates
Applications: ELISA Development -
Synergistic T cell signaling by 41BB and CD28 is optimally achieved by membrane proximal positioning within parallel chimeric antigen receptors
Authors: Tamara Muliaditan, Leena Halim, Lynsey M. Whilding, Benjamin Draper, Daniela Y. Achkova, Fahima Kausar et al.
Cell Reports Medicine
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Preclinical In Vivo Modeling of Cytokine Release Syndrome Induced by ErbB-Retargeted Human T Cells: Identifying a Window of Therapeutic Opportunity?
Authors: Sjoukje J. C. van der Stegen, David M. Davies, Scott Wilkie, Julie Foster, Jane K. Sosabowski, Jerome Burnet et al.
The Journal of Immunology
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