Home / Podocalyxin / Human Podocalyxin PE-conjugated Antibody

Human Podocalyxin PE-conjugated Antibody

Catalog #: FAB1658P
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB1658P
Detection of Podocalyxin in BG01V Human Embryonic Stem Cells by Flow Cytometry.
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Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Podocalyxin PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Podocalyxin in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse Podocalyxin or recombinant human Endoglycan is observed.
Source
Monoclonal Mouse IgG2A Clone # 222328
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Podocalyxin
Ser23-Arg427
Accession # AAB61574
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Podocalyxin antibody in BG01V Human Embryonic Stem Cells antibody by Flow Cytometry. View Larger

Detection of Podocalyxin in BG01V Human Embryonic Stem Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Mouse Anti-Human Podocalyxin PE-conjugated Monoclonal Antibody (Catalog # FAB1658P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Podocalyxin

Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology (1). Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors (2). It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells.

References
  1. Li, J. et al. (2001) DNA Seq. 12:407.
  2. Hara, T. et al. (1999) Immunity 11:567.
Entrez Gene IDs
5420 (Human); 27205 (Mouse)
Alternate Names
GCTM; Gp200MGC138240; PCLP; PCLP1; PCLP-1; PCLPGCTM-2 antigen; PCpodocalyxin; POD XL; Podocalyxin; Podocalyxin-like protein 1; podocalyxin-like; PODXL

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Citations for Human Podocalyxin PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Loss of KLF15 accelerates chronic podocyte injury
    Authors: Seung Seok Han, Mi‑Yeon Yu, Kyung Don Yoo, Jung Pyo Lee, Dong Ki Kim, Yon Su Kim et al.
    International Journal of Molecular Medicine
  2. Detection of urinary podocytes by flow cytometry in idiopathic membranous nephropathy
    Authors: A Mella, I Deambrosis, S Mingozzi, L Colla, M Burdese, F Giaretta, S Bruno, G Camussi, E Boaglio, C Dolla, R Clari, L Biancone
    Sci Rep, 2020-10-01;10(1):16362.
    Species: Human
    Sample Types: Urine
    Applications: ICC
  3. Podocalyxin promotes proliferation and survival in mature B-cell non-Hodgkin lymphoma cells
    Authors: E Tamayo-Orb, L Amo, M Riñón, N Nieto, E Amutio, N Maruri, M Solaun, A Arrieta, S Larrucea
    Oncotarget, 2017-09-27;8(59):99722-99739.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Regeneration of glomerular podocytes by human renal progenitors.
    Authors: Ronconi E, Sagrinati C, Angelotti ML
    J. Am. Soc. Nephrol., 2008-12-17;20(2):322-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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