Human Podocalyxin Antibody

Name: Human Podocalyxin Antibody
Brand: R&D Systems
SKU: MAB1658
Rating: 4.5 (6 reviews)

Catalog #: MAB1658
20 Citations 6 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1658
MAB1658-SP

Detection of Podocalyxin in BG01V Human Cells by Flow Cytometry.

2 Images

All Podocalyxin Antibodies

Product Details

Citations (20)

FAQs

Supplemental Products

Reviews (6)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Podocalyxin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Podocalyxin in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse Podocalyxin or recombinant human Endoglycan is observed.

Source

Monoclonal Mouse IgG_2A Clone # 222328

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Podocalyxin
Ser23-Arg427
Accession # AAB61574

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Podocalyxin

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of Podocalyxin in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells was stained with Mouse Anti-Human Podocalyxin Monoclonal Antibody (Catalog # MAB1658, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry

View Larger

Podocalyxin in BG01V Human Stem Cells. Podocalyxin was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Mouse Anti-Human Podocalyxin Monoclonal Antibody (Catalog # MAB1658) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Podocalyxin

Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology (1). Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors (2). It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells.

References

Li, J. et al. (2001) DNA Seq. 12:407.
Hara, T. et al. (1999) Immunity 11:567.

Entrez Gene IDs

5420 (Human); 27205 (Mouse)

Alternate Names

GCTM; Gp200MGC138240; PCLP; PCLP1; PCLP-1; PCLPGCTM-2 antigen; PCpodocalyxin; POD XL; Podocalyxin; Podocalyxin-like protein 1; podocalyxin-like; PODXL

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Podocalyxin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
Filter your results:

Filter by:

A single cell characterisation of human embryogenesis identifies pluripotency transitions and putative anterior hypoblast centre
Authors: Matteo A. Molè, Tim H. H. Coorens, Marta N. Shahbazi, Antonia Weberling, Bailey A. T. Weatherbee, Carlos W. Gantner et al.
Nature Communications
Podocalyxin Expressed in Antigen Presenting Cells Promotes Interaction With T Cells and Alters Centrosome Translocation to the Contact Site
Authors: Laura Amo, Javier Díez-García, Estíbaliz Tamayo-Orbegozo, Natalia Maruri, Susana Larrucea
Frontiers in Immunology
Modeling kidney development, disease, and plasticity with clonal expandable nephron progenitor cells and nephron organoids
Authors: Huang B, Zeng Z, Li H et al.
bioRxiv
PCDH12 loss results in premature neuronal differentiation and impeded migration in a cortical organoid model
Authors: Rakotomamonjy, J;Rylaarsdam, L;Fares-Taie, L;McDermott, S;Davies, D;Yang, G;Fagbemi, F;Epstein, M;Fairbanks-Santana, M;Rozet, JM;Guemez-Gamboa, A;
Cell reports
Species: Human
Sample Types: Whole Cells
Applications: ICC
Genome-wide screening in human kidney organoids identifies developmental and disease-related aspects of nephrogenesis
Authors: R Ungricht, L Guibbal, MC Lasbennes, V Orsini, M Beibel, A Waldt, R Cuttat, W Carbone, A Basler, G Roma, F Nigsch, JS Tchorz, D Hoepfner, PS Hoppe
Cell Stem Cell, 2021-11-29;0(0):.
Species: Human
Sample Types: Organoid
Applications: IHC
Comparative evaluation of isogenic mesodermal and ectomesodermal chondrocytes from human iPSCs for cartilage regeneration
Authors: MS Lee, MJ Stebbins, H Jiao, HC Huang, EM Leiferman, BE Walczak, SP Palecek, EV Shusta, WJ Li
Science Advances, 2021-05-19;7(21):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Naive stem cell blastocyst model captures human embryo lineage segregation
Authors: A Yanagida, D Spindlow, J Nichols, A Dattani, A Smith, G Guo
Cell Stem Cell, 2021-05-05;28(6):1016-1022.e4.
Species: Human
Sample Types: Embryo
Applications: ICC
Identification of the SHREK Family of Proteins as Broad-Spectrum Host Antiviral Factors
Authors: D Dabbagh, S He, B Hetrick, L Chilin, A Andalibi, Y Wu
Viruses, 2021-05-04;13(5):.
Species: Virus
Sample Types: Viral Particles
Applications: Immunoprecipitation
Identification of the SHREK family of proteins as broad-spectrum host antiviral factors
Authors: D Dabbagh, S He, B Hetrick, L Chilin, A Andalibi, Y Wu
bioRxiv : the preprint server for biology, 2021-02-03;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ELISA Capture, ICC
Developmental potential of aneuploid human embryos cultured beyond implantation
Authors: MN Shahbazi, T Wang, X Tao, BAT Weatherbee, L Sun, Y Zhan, L Keller, GD Smith, A Pellicer, RT Scott, E Seli, M Zernicka-G
Nat Commun, 2020-08-10;11(1):3987.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC

Podocalyxin promotes proliferation and survival in mature B-cell non-Hodgkin lymphoma cells
Authors: E Tamayo-Orb, L Amo, M Riñón, N Nieto, E Amutio, N Maruri, M Solaun, A Arrieta, S Larrucea
Oncotarget, 2017-09-27;8(59):99722-99739.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Self-organization of the human embryo in the absence of maternal tissues
Nat. Cell Biol., 2016-05-04;18(6):700-8.
Species: Human
Sample Types: Whole Cells
Applications: IHC

Podocalyxin-like protein is expressed in glioblastoma multiforme stem-like cells and is associated with poor outcome.
Authors: Binder Z, Siu I, Eberhart C, ap Rhys C, Bai R, Staedtke V, Zhang H, Smoll N, Piantadosi S, Piccirillo S, Dimeco F, Weingart J, Vescovi A, Olivi A, Riggins G, Gallia G
PLoS ONE, 2013-10-16;8(10):e75945.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Characterization of renal progenitors committed toward tubular lineage and their regenerative potential in renal tubular injury.
Authors: Angelotti ML, Ronconi E, Ballerini L, Peired A, Mazzinghi B, Sagrinati C, Parente E, Gacci M, Carini M, Rotondi M, Fogo AB, Lazzeri E, Lasagni L, Romagnani P
Stem Cells, 2012-08-01;30(8):1714-25.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Notch activation differentially regulates renal progenitors proliferation and differentiation toward the podocyte lineage in glomerular disorders.
Authors: Lasagni L, Ballerini L, Angelotti ML
Stem Cells, 2010-09-01;28(9):1674-85.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr

Regeneration of glomerular podocytes by human renal progenitors.
Authors: Ronconi E, Sagrinati C, Angelotti ML
J. Am. Soc. Nephrol., 2008-12-17;20(2):322-32.
Species: Human
Sample Types: Whole Cells
Applications: Cell Selection

Human Embryonic Stem Cells Express Elevated Levels of Multiple Pro-Apoptotic BCL-2 Family Members
Authors: David T. Madden, Diana Davila-Kruger, Simon Melov, Dale E. Bredesen
PLoS ONE

An apicosome initiates self-organizing morphogenesis of human pluripotent stem cells
Authors: Kenichiro Taniguchi, Yue Shao, Ryan F. Townshend, Chari L. Cortez, Clair E. Harris, Sasha Meshinchi et al.
Journal of Cell Biology

Effect of Cell Spreading on Rosette Formation by Human Pluripotent Stem Cell-Derived Neural Progenitor Cells
Authors: Ryan F. Townshend, Yue Shao, Sicong Wang, Chari L. Cortez, Sajedeh Nasr Esfahani, Jason R. Spence et al.
Frontiers in Cell and Developmental Biology