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Human Phospho-PGC1 alpha (S571) Antibody

Catalog #: AF6650
13 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF6650
AF6650-SP
Detection of Human Phospho-PGC1 alpha (S571) by Western Blot.
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Citations (13)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-PGC1 alpha (S571) Antibody Summary

Species Reactivity
Human
Specificity
Detects human PGC1 alpha when phosphorylated at S571 in Western blots.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide containing the human PGC1 alpha S571 site.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Phospho-PGC1a (S571) antibody by Western Blot. View Larger

Detection of Human Phospho-PGC1 alpha (S571) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 10 nm Recombinant Human IGF-I (Catalog # 291-G1) for 1 hour. PVDF Membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-PGC1a (S571) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6650) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-PGC1a (S571) at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PGC1 alpha

PGC-1 alpha (PPAR-gamma coactivator 1; also LEM6) is a 97-120 kDa member of the PGC-1 family of proteins. It is expressed in select cell types, including brown adipocytes, skeletal muscle and hepatocytes. PGC-1 alpha participates in both RNA processing and transcriptional coactivation in conjunction with multiple nuclear hormone receptors such as PPAR gamma, RAR and TR. Human PCG-1 alpha is 798 amino acids (aa) in length. It contains an LxxLL nuclear receptor binding motif (aa 144-148), one PPAR-gamma interaction domain (aa 293-339), two NLSs and an RNA binding/processing region (aa 566-710). PGC-1 alpha activity is regulated by phosphorylation. AMPK is known to phosphorylate Thr178 and Ser539, promoting cotranscriptional activity. Conversely, Akt-mediated phosphorylation at Ser571 is reported to downregulate PGC-1 alpha activity. This latter effect is achieved by an initial Ser571 phosphorylation, followed by GCN5 binding and subsequent PCG-1 alpha acetylation that promotes PGC-1 alpha dissociation from target gene promoters.

Long Name
Peroxisome Proliferator-activated Receptor gamma, Coactivator 1 alpha
Entrez Gene IDs
10891 (Human); 19017 (Mouse); 83516 (Rat)
Alternate Names
LEM6; Ligand effect modulator 6; ligand effect modulator-6; L-PGC-1alpha; peroxisome proliferative activated receptor, gamma, coactivator 1, alpha; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-3ext; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-8a; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B5-NT; peroxisome proliferator-activated receptor gamma coactivator 1-alpha; peroxisome proliferator-activated receptor gamma, coactivator 1 alpha; PGC1 alpha; PGC1; PGC-1(alpha); PGC1A; PGC-1-alpha; PGC1APGC-1(alpha); PGC1peroxisome proliferative activated receptor, gamma, coactivator 1; PGC-1v; PPAR gamma coactivator variant form; PPAR gamma coactivator-1; PPARgamma coactivator 1alpha; PPAR-gamma coactivator 1-alpha; PPARGC1; PPARGC1A; PPARGC-1-alpha

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Citations for Human Phospho-PGC1 alpha (S571) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Regulatory chromatin rewiring promotes metabolic switching during adaptation to oncogenic receptor tyrosine kinase inhibition
    Authors: S Ogden, K Carys, I Ahmed, J Bruce, AD Sharrocks
    Oncogene, 2022-09-24;0(0):.
  2. Caloric restriction increases the resistance of aged heart to myocardial ischemia/reperfusion injury via modulating AMPK-SIRT1-PGC1a energy metabolism pathway
    Authors: Guo Z, Wang M, Ying X et al.
    Scientific reports
  3. Inorganic nitrate and nitrite ameliorate kidney fibrosis by restoring lipid metabolism via dual regulation of AMP-activated protein kinase and the AKT-PGC1alpha pathway
    Authors: X Li, Z Zhuge, LRRA Carvalho, VA Braga, RB Lucena, S Li, TA Schiffer, H Han, E Weitzberg, JO Lundberg, M Carlström
    Redox Biology, 2022-02-17;51(0):102266.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. AMPK modulation ameliorates dominant disease phenotypes of CTRP5 variant in retinal degeneration
    Authors: Kiyoharu J. Miyagishima, Ruchi Sharma, Malika Nimmagadda, Katharina Clore-Gronenborn, Zoya Qureshy, Davide Ortolan et al.
    Communications Biology
  5. Effects of whole-body vibration training in a cachectic C26 mouse model
    Authors: M van der En, RLC Plas, M van Dijk, JT Dwarkasing, F van Gemerd, A Sarokhani, HJM Swarts, EM van Schoth, S Grefte, RF Witkamp, K van Norren
    Scientific Reports, 2021-11-03;11(1):21563.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. Dyslipidemia, insulin resistance, and impairment of placental metabolism in the offspring of obese mothers
    Authors: Bucher M, Montaniel KRC, Myatt L et al.
    Journal of Developmental Origins of Health and Disease
  7. Post‐translational regulation of PGC‐1 alpha modulates fibrotic repair
    Authors: Jennifer L. Larson‐Casey, Linlin Gu, Dana Davis, Guo‐Qiang Cai, Qiang Ding, Chao He et al.
    The FASEB Journal
  8. Wnt/?-catenin/RAS signaling mediates age-related renal fibrosis and is associated with mitochondrial dysfunction
    Authors: J Miao, J Liu, J Niu, Y Zhang, W Shen, C Luo, Y Liu, C Li, H Li, P Yang, Y Liu, FF Hou, L Zhou
    Aging Cell, 2019-07-18;0(0):e13004.
    Species: Human, Mouse
    Sample Types: Cell Lysates, Tissue Homogenates
    Applications: Western Blot
  9. Chloroquine and amodiaquine enhance AMPK phosphorylation and improve mitochondrial fragmentation in diabetic tubulopathy
    Authors: HY Jeong, JM Kang, HH Jun, DJ Kim, SH Park, MJ Sung, JH Heo, DH Yang, SH Lee, SY Lee
    Sci Rep, 2018-06-08;8(1):8774.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Coordinated regulation of hepatic FoxO1, PGC-1? and SREBP-1c facilitates insulin action and resistance
    Authors: MP Sajan, MC Lee, F Foufelle, J Sajan, C Cleland, RV Farese
    Cell. Signal., 2017-12-18;43(0):62-70.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  11. NADPH Oxidase 4 (Nox4) Suppresses Mitochondrial Biogenesis and Bioenergetics in Lung Fibroblasts via a Nuclear Factor Erythroid-derived 2-like 2 (Nrf2)-dependent Pathway
    Authors: K Bernard, NJ Logsdon, V Miguel, GA Benavides, J Zhang, AB Carter, VM Darley-Usm, VJ Thannickal
    J. Biol. Chem, 2017-01-03;292(7):3029-3038.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Metabolic Reprogramming Is Required for Myofibroblast Contractility and Differentiation.
    Authors: Bernard K, Logsdon N, Ravi S, Xie N, Persons B, Rangarajan S, Zmijewski J, Mitra K, Liu G, Darley-Usmar V, Thannickal V
    J Biol Chem, 2015-08-28;290(42):25427-38.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. A locked immunometabolic switch underlies TREM2 R47H loss of function in human iPSC-derived microglia
    Authors: Piers TM, Cosker K, Mallach A et al.
    FASEB J.

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Human Phospho-PGC1 alpha (S571) Antibody
By Anonymous on 10/20/2021
Application: WB Sample Tested: HepG2 human hepatocellular carcinoma cell line Species: Human
Western Blot Human Phospho-PGC1 alpha (S571) Antibody AF6650