Human Phospho-EGFR (Y1173) Antibody

Catalog # Availability Size / Price Qty
AF1095
AF1095-SP
Detection of Human Phospho-EGFR (Y1173) by Western Blot.
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Product Details
Citations (10)
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Human Phospho-EGFR (Y1173) Antibody Summary

Species Reactivity
Human
Specificity
Detects human EGFR when phosphorylated at Y1173 in Western blots.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide containing human EGFR Y1173 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Simple Western
2 µg/mL
See below
Immunohistochemistry
0.5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Phospho-EGFR (Y1173) antibody by Western Blot. View Larger

Detection of Human Phospho-EGFR (Y1173) by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 100 µM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human Phospho-EGFR (Y1173) Antigen Affinity-purified Polyclonal Antibody, followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-EGFR (Y1173) at approximately 185 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Phospho-EGFR (Y1173) antibody in A431 Human Cell Line by Immunocytochemistry (ICC). View Larger

Phospho-EGFR (Y1173) in A431 Human Cell Line. EGFR phosphorylated at Y1173 was detected in immersion fixed A431 human epithelial carcinoma cell line untreated (lower panel) or treated (upper panel) with pervanadate using Rabbit Anti-Human Phospho-EGFR (Y1173) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1095) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Phospho-EGFR (Y1173) antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Phospho-EGFR (Y1173) in Mouse Embryo. EGFR phosphorylated at Y1173 was detected in immersion fixed frozen sections of mouse embryo using Rabbit Anti-Human Phospho-EGFR (Y1173) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1095) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunohistochemistry View Larger

EGFR in Human Lung Cancer. EGFR was detected in immersion fixed frozen sections of human lung cancer using Rabbit Anti-Human Phospho-EGFR (Y1173) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1095) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in cancer cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Intracellular Staining by Flow Cytometry Detection of Phospho-EGFR (Y1173) antibody in A431 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Phospho-EGFR (Y1173) in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cells were untreated (open histogram), or treated for 5 minutes with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG; filled histogram) then stained with Rabbit Anti-Human Phospho-EGFR (Y1173) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1095), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed with para-formaldehyde and permeabilized with saponin.

Simple Western Detection of Human Phospho-EGFR (Y1173) antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human Phospho-EGFR (Y1173) by Simple WesternTM. Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 10 ng/mL Recombinant Human EGF (Catalog # 236-EG) for 5 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-EGFR (Y1173) at approximately 265 kDa (as indicated) using 2 µg/mL of Rabbit Anti-Human Phospho-EGFR (Y1173) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1095). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EGFR

Epidermal growth factor receptor (EGFR, also known as ErbB1 and HER1) is the founding member of the ErbB family of receptor tyrosine kinases. Ligand binding induces receptor dimerization and autophosphorylation on multiple tyrosine residues. Phosphorylation of Y1173 creates a binding site for the protein tyrosine phosphatase SHP-1, leading to attenuation of receptor signaling.

Long Name
Epidermal Growth Factor Receptor
Entrez Gene IDs
1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)
Alternate Names
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC 2.7.10.1; EGF R; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

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Citations for Human Phospho-EGFR (Y1173) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Piezo1 activates noncanonical EGFR endocytosis and signaling
    Authors: Carlos Pardo-Pastor, Jody Rosenblatt
    Sci Adv
  2. Cell Derived Matrix Fibulin-1 Associates With Epidermal Growth Factor Receptor to Inhibit Its Activation, Localization and Function in Lung Cancer Calu-1 Cells
    Authors: Keerthi Harikrishnan, Omkar Joshi, Saili Madangirikar, Nagaraj Balasubramanian
    Frontiers in Cell and Developmental Biology
  3. Amivantamab (JNJ-61186372), an Fc Enhanced EGFR/cMet Bispecific Antibody, Induces Receptor Downmodulation and Antitumor Activity by Monocyte/Macrophage Trogocytosis.
    Authors: Vijayaraghavan S, Lipfert L et al.
    Mol Cancer Ther
    Species: Human
    Sample Types:
    Applications: Simple Western
  4. DNA damage alters EGFR signaling and reprograms cellular response via Mre-11
    Authors: Y Volman, R Hefetz, E Galun, J Rachmilewi
    Scientific Reports, 2022-04-06;12(1):5760.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. EGFR phosphorylates HDAC1 to regulate its expression and anti-apoptotic function
    Authors: S Bahl, H Ling, NPN Acharige, I Santos-Bar, MKH Pflum, E Seto
    Cell Death & Disease, 2021-05-11;12(5):469.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, IP, Western Blot
  6. ADAM17 substrate release in proximal tubule drives kidney fibrosis
    JCI Insight, 2016-08-18;1(13):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Phenotypic diversity of breast cancer-related mutations in metalloproteinase-disintegrin ADAM12.
    Authors: Qi, Yue, Duhachek-Muggy, Sara, Li, Hui, Zolkiewska, Anna
    PLoS ONE, 2014-03-20;9(3):e92536.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  8. Analysis of Epithelial Growth Factor-Receptor (EGFR) Phosphorylation in Uterine Smooth Muscle Tumors: Correlation to Mucin-1 and Galectin-3 Expression.
    Authors: Weissenbacher, Tobias, Vrekoussis, Thomas, Roeder, David, Makrigiannakis, Antonis, Mayr, Doris, Ditsch, Nina, Friese, Klaus, Jeschke, Udo, Dian, Darius
    Int J Mol Sci, 2013-02-28;14(3):4783-92.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. Repulsion of cerebellar granule neurons by chondroitin sulfate proteoglycans is mediated by MAPK pathway.
    Authors: Kaneko M, Kubo T, Hata K, Yamaguchi A, Yamashita T
    Neurosci. Lett., 2007-07-05;423(1):62-7.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Protein C is an autocrine growth factor for human skin keratinocytes.
    Authors: Xue M, Campbell D, Jackson CJ
    J. Biol. Chem., 2007-02-09;282(18):13610-6.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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