Human PER1 Antibody

Catalog #: AF4565 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4565
AF4565-SP

All PER1 Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PER1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human PER1 in direct ELISAs.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PER1
Ser159-Thr365
Accession # O15534

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of human brain (cortex and hypothalamus)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PER1

PER1 (Period circadian protein 1; also known as RIGUI) is a 188 kDa member of the PER family of molecules. It apparently influences circadian rhythms by interacting with, and stabilizing, other circadian regulatory proteins. PER1 undergoes extensive phosphorylation that may regulate its intracellular localization. Human PER1 is 1290 amino acids (aa) in length. It contains a bHLH region (aa 122-173), an extended PAS domain (aa 183-463) plus an NLS and NES. Multiple splice forms exist. Two show deletions (aa 741-820 and aa 209-217), while two show aa substitutions: 9 aa for the C-terminal 1005 aa, and 29 aa for the C-terminal 747 amino acids. Over aa 159-365, human PER1 is 96% identical to mouse, rat, and dog PER1.

Long Name

Period Circadian Protein 1

Entrez Gene IDs

5187 (Human); 18626 (Mouse)

Alternate Names

Circadian clock protein PERIOD 1; Circadian pacemaker protein Rigui; hPER1; KIAA0482; PER1; PERhPER; period (Drosophila) homolog 1; period circadian protein homolog 1; period homolog 1 (Drosophila); Period, drosophila, homolog of; PERIOD1; RIGUI; RIGUIMGC88021