Human NF-H Antibody

Catalog #: MAB3108 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3108
MAB3108-SP

All NF-H Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NF-H Antibody Summary

Species Reactivity

Human

Specificity

Detects human NF-H in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) NF-L or rhNF-M is observed.

Source

Monoclonal Mouse IgG₁ Clone # 321721

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human NF-H
Met1-Ala380
Accession # P12036

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human NF-H

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NF-H

The human Neurofilament Heavy chain, also known as NF-H and NEFH, is a phosphorylated cytoskeletal intermediate filament protein that is expressed in neurons. Neurofilaments are trimers that always contain the 68 kDa NF-L and variably contain 125 kDa NF-M and 200 kDa NF-H. In the region used for immunization, mouse and rat NF-H are each 93% identical to human NF-H.

Long Name

Neurofilament Protein, Heavy Chain

Entrez Gene IDs

4744 (Human)

Alternate Names

200 kDa neurofilament protein; KIAA0845; NEFH; Neurofilament Heavy (200kDa); neurofilament heavy polypeptide; Neurofilament triplet H protein; neurofilament, heavy polypeptide 200kDa; neurofilament, heavy polypeptide; NF200; NFH; NF-H