Human NF-H Antibody

Catalog #: MAB31082 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB31082-100
MAB31082-SP

Detection of Human NF‑H by Western Blot.

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All NF-H Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NF-H Antibody Summary

Species Reactivity

Human

Specificity

Detects human NF-H in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 1016103

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human NF-H peptide
Accession # P12036

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

IMR‑32 human neuroblastoma cell line

Simple Western

5 µg/mL

Human motor cortex and human hippocampus

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human NF‑H by Western Blot. Western blot shows lysates of IMR‑32 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human NF‑H Monoclonal Antibody (Catalog # MAB31082) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for NF‑H at approximately 210 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western

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Detection of Human NF‑H by Simple Western^TM. Simple Western shows lysates of human motor cortex and human hippocampus, loaded at 0.2 mg/ml. A specific band was detected for NF‑H at approximately 270-350 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human NF‑H Monoclonal Antibody (Catalog # MAB31082). This experiment was conducted under reducing conditions and using the 66-440kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NF-H

The human Neurofilament Heavy chain, also known as NF-H and NEFH, is a phosphorylated cytoskeletal intermediate filament protein that is expressed in neurons. Neurofilaments are trimers that always contain the 68 kDa NF-L and variably contain 125 kDa NF-M and 200 kDa NF-H. In the region used for immunization, mouse and rat NF-H are each 93% identical to human NF-H.

Long Name

Neurofilament Protein, Heavy Chain

Entrez Gene IDs

4744 (Human)

Alternate Names

200 kDa neurofilament protein; KIAA0845; NEFH; Neurofilament Heavy (200kDa); neurofilament heavy polypeptide; Neurofilament triplet H protein; neurofilament, heavy polypeptide 200kDa; neurofilament, heavy polypeptide; NF200; NFH; NF-H