Human Nephrin Antibody

Catalog #: MAB42692 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB42692-100
MAB42692-SP

Detection of Human Nephrin by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Nephrin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Nephrin in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 1037720

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived human Nephrin
Gln23-Thr1029
Accession # O60500

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

HEK293T human embryonic kidney cell line and human kidney (medulla)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Nephrin by Western Blot. Western blot shows lysates of HEK293T human embryonic kidney cell line and human kidney (medulla). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Nephrin Monoclonal Antibody (Catalog # MAB42692) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Nephrin at approximately 134 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nephrin

Nephrin (or renal glomerulus-specific cell adhesion receptor; also NPHS1) is a 185 - 200 kDa member of the Ig superfamily. It is expressed on glomerular podocyte membranes, associates with same-cell Neph1, and forms homomeric zipper-like complexes with Nephrin on adjacent podocytes. Mature human Nephrin is a type I transmembrane glycoprotein 1219 amino acids (aa) in length. It contains a 1033 aa extracellular region and a 165 aa cytoplasmic tail. One potential, soluble splice form is known where aa’s 1056 - 1095 are deleted, eliminating the transmembrane region. Over aa 23 - 1029, human Nephrin shares 84% and 89% aa identity with mouse and dog Nephrin, respectively.

Entrez Gene IDs

4868 (Human); 54631 (Mouse); 64563 (Rat)

Alternate Names

CNF; Nephrin; nephrosis 1, congenital, Finnish type (nephrin); NPHNCNF; NPHS1; Renal glomerulus-specific cell adhesion receptor