Human/Mouse UBE2K/E2-25K Antibody

Catalog #: MAB6609
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6609
MAB6609-SP

Detection of Human UBE2K/E2‑25K by Western Blot.

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse UBE2K/E2-25K Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human UBE2K/E2‑25K in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 701316

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human UBE2K/E2‑25K
Ala2-Asn200
Accession # P61086

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human UBE2K/E2‑25K by Western Blot. Western blot shows lysates of human brain (cortex) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human UBE2K/E2-25K Monoclonal Antibody (Catalog # MAB6609) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for UBE2K/E2-25K at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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UBE2K/E2‑25K in Human Brain. UBE2K/ E2-25K was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Mouse Anti-Human UBE2K/E2-25K Monoclonal Antibody (Catalog # MAB6609) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: UBE2K/E2-25K

UBE2K (Ubiquitin-conjugating enzyme E2; also E2-25K, HIP-2, UBE2K-25K and LIG) is a 24-26 kDa cytosolic member of the UBE family of proteins. It is widely expressed (in neurons, monocytes and neutrophils), and acts to ubiquitinate proteins targeted for degradation. UBE2K serves as an E2 ubiquitin conjugating enzyme with a preference for ubiquitin chain generation through Lys48. It appears to polyubiquitinate targets once they have been monoubiquitinated, and to act as either a monomer or dimer. Human UBE2K is 200 amino acids (aa) in length. It contains one UBA domain (aa 160-200). There are two potential splice variants that show a deletion of aa 22-72 and 134-176, respectively. Human and mouse UBE2K are identical in aa sequence.

Long Name

Ubiquitin-conjugating Enzyme E2

Entrez Gene IDs

3093 (Human); 53323 (Mouse); 289623 (Rat)

Alternate Names

E2(25K); E225K; E2-25K; EC 6.3.2.19; Hip2; HIP-2; huntingtin interacting protein 2; Huntingtin-interacting protein 2; HYPG; LIG; UbcH1; UBE2K; UBE2K-25K; ubiquitin carrier protein; ubiquitin-conjugating enzyme E2 K; Ubiquitin-conjugating enzyme E2(25K); Ubiquitin-conjugating enzyme E2-25 kDa; Ubiquitin-conjugating enzyme E2-25K; ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast); ubiquitin-protein ligase

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human/Mouse UBE2K/E2-25K Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Proteasome proteolysis supports stimulated platelet function and thrombosis.
Authors: Gupta N, Li W, Willard B, Silverstein R, McIntyre T
Arterioscler Thromb Vasc Biol, 2013-10-31;34(1):160-8.
Species: Human
Sample Types: Whole Blood
Applications: Western Blot