Human/Mouse TLR9 Antibody

Catalog #: MAB36581 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB36581-100
MAB36581-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse TLR9 Antibody Summary

Specificity

Detects human TLR9 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 980915

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human TLR9 synthetic peptide
Accession # Q9NR96

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

1-25 µg/mL

See below

Immunocytochemistry

3-25 µg/mL

Immersion fixed mouse splenocytes, Daudi Human Burkitt's Lymphoma Cell Line (positive) and HDLM‑2 Human Hodgkin's Lymphoma Cell Line (negative) Cells.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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TLR9 in Mouse Splenocytes. TLR9 was detected in immersion fixed mouse splenocytes using Mouse Anti-Human TLR9 Monoclonal Antibody (Catalog # MAB36581) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry

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TLR9 in Mouse Spleen. TLR9 was detected in perfusion fixed frozen sections of mouse spleen using Mouse Anti-Human TLR9 Monoclonal Antibody (Catalog # MAB36581) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunocytochemistry

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Detection of TLR9 in Daudi Human Burkitt's Lymphoma Cell Line (positive) and HDLM‑2 Human Hodgkin’s Lymphoma Cell Line (negative). TLR9 was detected in immersion fixed Daudi Human Burkitt's Lymphoma Cell Line (positive) and absent in HDLM‑2 Human Hodgkin’s Lymphoma Cell Line (negative) Cells using Mouse Anti-Human/Mouse TLR9 Monoclonal Antibody (Catalog # MAB36581) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLR9

TLR9 (Toll receptor 9; also CD289) is a 145-150 kDa member of the Toll-like receptor family of molecules. It is expressed by colonic epithelium, CD123⁺ plasmacytoid dendritic cells, and transitional B cells, and responds to unmethylated DNA CpG motifs that contain either a GTCGTT sequence (in human), or a GACGTT sequence (in mouse). TLR9 is found in the ER and translocates to either the cell membrane or to lysosomes where it binds bacterial DNA. Precursor human TLR9 is a type I transmembrane protein 1032 amino acids (aa) in length. It possesses a 793 aa extracellular region that contains 26 LRRs (aa 26-818) plus a 193 aa cytoplasmic domain. The full-length 150 kDa form is suggested to be ligand-binding but nonsignaling. The active form is believed to be an 80 kDa cleavage product found in the endosome compartment. There are multiple splice forms. One contains a deletion of aa 2-16, a second possesses an alternate start site at Met58, while a third and fourth show alternative start sites aa 23 and 24 upstream of the standard site. Over aa 64-189, human TLR9 shares 76% aa identity with mouse TLR9.

Long Name

Toll-like Receptor 9

Entrez Gene IDs

54106 (Human); 81897 (Mouse); 338457 (Rat)

Alternate Names

CD289 antigen; CD289; TLR9; toll-like receptor 9