Home / Tenascin C / Human/Mouse Tenascin C Antibody

Human/Mouse Tenascin C Antibody

Catalog #: MAB2138
29 Citations 2 Reviews Datasheet / COA / SDS
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MAB2138
MAB2138-SP
Tenascin C in U‑118 MG Human Cell Line.
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Citations (29)
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Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Tenascin C Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detect human and mouse Tenascin C in Western blots.
Source
Monoclonal Rat IgG2A Clone # 578
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse immature astrocyte-derived Tenascin C
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
Morganti, M. et al. (1990) Exp. Neurol. 109:98.
 
Immunocytochemistry
8-25 µg/mL
See below
Neutralization
Husmann, K. et al. (1992) J. Cell Biol. 116:1475.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Tenascin C antibody in U-118 MG Human Cell Line by Immunocytochemistry (ICC). View Larger

Tenascin C in U‑118 MG Human Cell Line. Tenascin C was detected in immersion fixed U-118 MG human glioblastoma/astrocytoma cell line using Rat Anti-Human/Mouse Tenascin C Monoclonal Antibody (Catalog # MAB2138) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tenascin C

Tenascin C, also known as hexabrachion, cytotactin, neuronectin, GMEM, JI, myotendinous antigen, glioma-associated-extracellular matrix antigen, and GP 150‑225, is a member of the Tenascin family of extracellular matrix proteins. It is secreted as a disulfide-linked homohexamer whose subunits can vary in size from approximately 200 kDa to over 300 kDa due to differences in glycosylation (1). Rotary-shadowed electron micrographs of the purified molecule show six strands joined to one another at one end in a globular domain with each arm terminating in a knob-like structure (2, 3). The human Tenascin C monomer is synthesized as a precursor with a 22 amino acid (aa) signal sequence and a 2179 aa mature chain. The mature chain consists of a coiled-coil region (aa 118‑145), followed by
15 EGF‑like domains, 15 fibronectin type-III domains, and a fibrinogen C-terminal domain. In addition, there are 23 potential sites of N‑linked glycosylation. Alternative splicing within the fibronectin type-III repeats produces six isoforms for human Tenascin C. Mature human Tenascin C (isoform 1) shares 84% aa sequence identity with mature mouse Tenascin C. In the developing embryo, Tenascin C is expressed during neural, skeletal, and vascular morphogenesis (1, 2). In the adult, it virtually disappears with continued basal expression detectable only in tendon-associated tissues (1, 2). However, great up-regulation in expression occurs in tissues undergoing remodeling processes seen during wound repair and neovascularization or in pathological states such as inflammation or tumorigenesis (1, 4, 5). Biologically, Tenascin C functions as an adhesion-modulatory extracellular matrix protein (1, 4‑8). Specifically, it antagonizes the adhesive effects of fibronectin, and impacts the ability of fibroblasts to deposit and contract the matrix by affecting the morphology and signaling pathways of adherent cells (5‑7). Tenascin C acts by blocking syndecan-4 binding at the edges of the wound and by suppressing fibronectin-mediated activation of RhoA and focal adhesion kinase (FAK) (4‑8). Tenascin C thus promotes epidermal cell migration and proliferation during wound repair.

References
  1. Hsia, H.C. and J.E. Schwarzbauer (2005) J. Biol. Chem. 280:26641.
  2. Nies, D.E. et al. (1991) J. Biol. Chem. 266:2818.
  3. Erickson, H.P and J.L. Iglesias (1984) Nature 311:267.
  4. Orend, G. et al. (2003) Oncogene 22:3917.
  5. Wenk, M.B. et al. (2000) J. Cell Biol. 150:913.
  6. Midwood, K.S. et al. (2004) Mol. Biol. Cell 15:5670.
  7. Midwood, K.S. and J. E. Schwarzbauer (2002) Mol. Biol. Cell 13:3601.
  8. Hsia, H.C. and J.E. Schwarzbauer (2006) J. Surg. Res. 136:92.
Entrez Gene IDs
3371 (Human); 21923 (Mouse); 116640 (Rat)
Alternate Names
150-225; Cytotactin; Glioma-associated-extracellular matrix antigen; GMEM; GP 150-225; hexabrachion (tenascin C, cytotactin); hexabrachion (tenascin); Hexabrachion; HXB; HXBcytotactin; JI; MGC167029; Myotendinous antigen; neuronectin; Tenascin C; Tenascin J1; tenascin; tenascin-C isoform 14/AD1/16; Tenascin-C; TNC; TN-C; TNGP

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Citations for Human/Mouse Tenascin C Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

29 Citations: Showing 1 - 10
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  1. Fibroblast activation and abnormal extracellular matrix remodelling as common hallmarks in three cancer‐prone genodermatoses
    Authors: E. Chacón‐Solano, C. León, F. Díaz, F. García‐García, M. García, M.J. Escámez et al.
    British Journal of Dermatology
  2. The Matrikine Tenascin-C Protects Multipotential Stromal Cells/Mesenchymal Stem Cells from Death Cytokines Such as FasL
    Authors: Melanie Rodrigues, Cecelia C. Yates, Austin Nuschke, Linda Griffith, Alan Wells
    Tissue Engineering Part A
  3. Regulation of IL-6 Secretion by Astrocytes via TLR4 in the Fragile X Mouse Model
    Authors: Victoria Krasovska, Laurie C. Doering
    Frontiers in Molecular Neuroscience
  4. CAQK, a peptide associating with extracellular matrix components targets sites of demyelinating injuries
    Authors: Charly Abi-Ghanem, Deepa Jonnalagadda, Jerold Chun, Yasuyuki Kihara, Barbara Ranscht
    Frontiers in Cellular Neuroscience
  5. Scaffold-free 3D cell culture of primary skin fibroblasts induces profound changes of the matrisome
    Authors: Bich Vu, Glauco R. Souza, Jörn Dengjel
    Matrix Biology Plus
  6. Proteomic Analysis of Laser Microdissected Melanoma Cells from Skin Organ Cultures
    Authors: Brian L. Hood, Jelena Grahovac, Melanie S. Flint, Mai Sun, Nuno Charro, Dorothea Becker et al.
    Journal of Proteome Research
  7. Tenascin-C-mediated suppression of extracellular matrix adhesion force promotes entheseal new bone formation through activation of Hippo signalling in ankylosing spondylitis
    Authors: Zihao Li, Siwen Chen, Haowen Cui, Xiang Li, Dongying Chen, Wenjun Hao et al.
    Annals of the Rheumatic Diseases
  8. Tenascin-C promotes angiogenesis in fibrovascular membranes in eyes with proliferative diabetic retinopathy
    Authors: Yoshiyuki Kobayashi, Shigeo Yoshida, Yedi Zhou, Takahito Nakama, Keijiro Ishikawa, Mitsuru Arima et al.
    Mol. Vis
  9. Global remodelling of cellular microenvironment due to loss of collagen VII
    Authors: Victoria Küttner, Claudia Mack, Kristoffer T G Rigbolt, Johannes S Kern, Oliver Schilling, Hauke Busch et al.
    Molecular Systems Biology
  10. Mesothelial cells with mesenchymal features enhance peritoneal dissemination by forming a protumorigenic microenvironment
    Authors: Yonemura, A;Semba, T;Zhang, J;Fan, Y;Yasuda-Yoshihara, N;Wang, H;Uchihara, T;Yasuda, T;Nishimura, A;Fu, L;Hu, X;Wei, F;Kitamura, F;Akiyama, T;Yamashita, K;Eto, K;Iwagami, S;Iwatsuki, M;Miyamoto, Y;Matsusaki, K;Yamasaki, J;Nagano, O;Saya, H;Song, S;Tan, P;Baba, H;Ajani, JA;Ishimoto, T;
    Cell reports
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  11. Mechanical tension mobilizes Lgr6+ epidermal stem cells to drive skin growth
    Authors: Y Xue, C Lyu, A Taylor, A Van Ee, A Kiemen, Y Choi, N Khavanian, D Henn, C Lee, L Hwang, E Wier, S Wang, S Lee, A Li, C Kirby, G Wang, PH Wu, D Wirtz, LA Garza, SK Reddy
    Science Advances, 2022-04-27;8(17):eabl8698.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC/IF
  12. Intestinal fibroblastic reticular cell niches control innate lymphoid cell homeostasis and function
    Authors: HW Cheng, U Mörbe, M Lütge, C Engetschwi, L Onder, M Novkovic, C Gil-Cruz, C Perez-Shib, T Hehlgans, E Scandella, B Ludewig
    Nature Communications, 2022-04-19;13(1):2027.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Pro-inflammatory immunity supports fibrosis advancement in epidermolysis bullosa: intervention with Ang-(1-7)
    Authors: R Bernasconi, K Thriene, E Romero-Fer, C Gretzmeier, T Kühl, M Maler, P Nauroy, S Kleiser, AC Rühl-Muth, M Stumpe, D Kiritsi, SF Martin, B Hinz, L Bruckner-T, J Dengjel, A Nyström
    Embo Molecular Medicine, 2021-08-30;0(0):e14392.
    Species: Human, Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  14. Capturing human trophoblast development with naive pluripotent stem cells in�vitro
    Authors: S Io, M Kabata, Y Iemura, K Semi, N Morone, A Minagawa, B Wang, I Okamoto, T Nakamura, Y Kojima, C Iwatani, H Tsuchiya, B Kaswandy, E Kondoh, S Kaneko, K Woltjen, M Saitou, T Yamamoto, M Mandai, Y Takashima
    Cell Stem Cell, 2021-04-07;28(6):1023-1039.e13.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC
  15. Expansion and characterization of epithelial stem cells with potential for cyclical hair regeneration
    Authors: M Takeo, K Asakawa, KE Toyoshima, M Ogawa, J Tong, T Irié, M Yanagisawa, A Sato, T Tsuji
    Scientific Reports, 2021-02-10;11(1):1173.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  16. Autophagy deficiency promotes triple-negative breast cancer resistance to T cell-mediated cytotoxicity by blocking tenascin-C degradation
    Authors: ZL Li, HL Zhang, Y Huang, JH Huang, P Sun, NN Zhou, YH Chen, J Mai, Y Wang, Y Yu, LH Zhou, X Li, D Yang, XD Peng, GK Feng, J Tang, XF Zhu, R Deng
    Nat Commun, 2020-07-30;11(1):3806.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay, Neutralization
  17. Transforming Growth Factor-Beta and Sonic Hedgehog Signaling in Palatal Epithelium Regulate Tenascin-C Expression in Palatal Mesenchyme During Soft Palate Development
    Authors: S Ohki, K Oka, K Ogata, S Okuhara, M Rikitake, M Toda-Nakam, S Tamura, M Ozaki, S Iseki, T Sakai
    Front Physiol, 2020-06-04;11(0):532.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. Single-cell analysis of progenitor cell dynamics and lineage specification in the human fetal kidney.
    Authors: Menon R, Otto E, Kokoruda A, Zhou J, Zhang Z, Yoon E, Chen Y, Troyanskaya O, Spence J, Kretzler M, Cebrian C
    Development, 2018-08-30;145(16):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  19. Combined CSL and p53 downregulation promotes cancer-associated fibroblast activation.
    Authors: Procopio M, Laszlo C, Al Labban D, Kim D, Bordignon P, Jo S, Goruppi S, Menietti E, Ostano P, Ala U, Provero P, Hoetzenecker W, Neel V, Kilarski W, Swartz M, Brisken C, Lefort K, Dotto G
    Nat Cell Biol, 2015-08-24;17(9):1193-204.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. Temporal expression of growth factors triggered by epiregulin regulates inflammation development.
    Authors: Harada M, Kamimura D, Arima Y, Kohsaka H, Nakatsuji Y, Nishida M, Atsumi T, Meng J, Bando H, Singh R, Sabharwal L, Jiang J, Kumai N, Miyasaka N, Sakoda S, Yamauchi-Takihara K, Ogura H, Hirano T, Murakami M
    J Immunol, 2015-01-02;194(3):1039-46.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  21. The missense mutation p.R1303Q in type XVII collagen underlies junctional epidermolysis bullosa resembling Kindler syndrome.
    Authors: Has, Cristina, Kiritsi, Dimitra, Mellerio, Jemima E, Franzke, Claus-We, Wedgeworth, Emma, Tantcheva-Poor, Iliana, Kernland-Lang, Kristin, Itin, Peter, Simpson, Michael, Dopping-Hepenstal, Patricia, Fujimoto, Wataru, McGrath, John A, Bruckner-Tuderman, Leena
    J Invest Dermatol, 2013-09-04;134(3):845-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  22. Melanoma cell invasiveness is promoted at least in part by the epidermal growth factor-like repeats of tenascin-C.
    Authors: Grahovac, Jelena, Becker, Dorothea, Wells, Alan
    J Invest Dermatol, 2012-09-06;133(1):210-20.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: IHC, Western Blot
  23. Mechanisms of fibroblast cell therapy for dystrophic epidermolysis bullosa: high stability of collagen VII favors long-term skin integrity.
    Authors: Kern JS, Loeckermann S, Fritsch A, Hausser I, Roth W, Magin TM, Mack C, Muller ML, Paul O, Ruther P, Bruckner-Tuderman L
    Mol. Ther., 2009-06-30;17(9):1605-15.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  24. ELR-negative CXC chemokine CXCL11 (IP-9/I-TAC) facilitates dermal and epidermal maturation during wound repair.
    Authors: Yates CC, Whaley D, Y-Chen A, Kulesekaran P, Hebda PA, Wells A
    Am. J. Pathol., 2008-07-31;173(3):643-52.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  25. A hypomorphic mouse model of dystrophic epidermolysis bullosa reveals mechanisms of disease and response to fibroblast therapy.
    Authors: Fritsch A, Loeckermann S, Kern JS, Braun A, Bosl MR, Bley TA, Schumann H, von Elverfeldt D, Paul D, Erlacher M, Berens von Rautenfeld D, Hausser I, Fassler R, Bruckner-Tuderman L
    J. Clin. Invest., 2008-05-01;118(5):1669-79.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  26. Essential role of Smad3 in infarct healing and in the pathogenesis of cardiac remodeling.
    Authors: Bujak M, Ren G, Kweon HJ, Dobaczewski M, Reddy A, Taffet G, Wang XF, Frangogiannis NG
    Circulation, 2007-10-22;116(19):2127-38.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  27. Adipose tissue derived stem cells differentiate into carcinoma-associated fibroblast-like cells under the influence of tumor derived factors
    Authors: Constantin Jotzu, Eckhard Alt, Gabriel Welte, Jie Li, Bryan T. Hennessy, Eswaran Devarajan et al.
    Cell Oncol (Dordr)
  28. Human Subacromial Bursal Cells Display Superior Engraftment Versus Bone Marrow Stromal Cells in Murine Tendon Repair
    Authors: Felix Dyrna, Philip Zakko, Leo Pauzenberger, Mary Beth McCarthy, Augustus D. Mazzocca, Nathaniel A. Dyment et al.
    The American Journal of Sports Medicine
  29. Genetic Background is a Key Determinant of Glomerular Extracellular Matrix Composition and Organization
    Authors: Michael J. Randles, Adrian S. Woolf, Jennifer L. Huang, Adam Byron, Jonathan D. Humphries, Karen L. Price et al.
    Journal of the American Society of Nephrology

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Human/Mouse Tenascin C Antibody
By Anonymous on 11/23/2020
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Melanoma tissue Species: Human
Immunocytochemistry/Immunofluorescence Human/Mouse Tenascin C Antibody MAB2138
Human/Mouse Tenascin C Antibody
By cristian De Gregorio on 11/11/2020
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Skin tissue Species: Mouse

Skin tissue derived from a mouse model of epidermolysis bullosa. The skin displays a splitting in the dermal-epidermal junction, and an exacerbated tenascin C deposition in the dermis.

Immunocytochemistry/Immunofluorescence Human/Mouse Tenascin C Antibody MAB2138