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Human/Mouse TAB1 Antibody

Catalog #: AF3578
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3578
AF3578-SP
Detection of Human/Mouse TAB1 by Western Blot.
2 Images
Product Details
Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse TAB1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse TAB1.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human TAB1
Ser7-Glu159
Accession # Q15750
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse TAB1 antibody by Western Blot. View Larger

Detection of Human/Mouse TAB1 by Western Blot. Western blot shows lysates of WS-1 human fetal skin fibroblast cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse TAB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3578) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TAB1 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry TAB1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

TAB1 in HeLa Human Cell Line. TAB1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse TAB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3578) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TAB1

TAK1-binding protein 1 (TAB1), also known as MAP3K7IP1, is an activator of the MAP3K TAK1. The C-terminal 68 amino acids of TAB1 are sufficient for binding and activation of TAK1, while a portion of the N-terminus acts as a dominant-negative inhibitor of TGF-beta, suggesting that TAB1 may function as a signaling intermediate between TGF-beta receptors and TAK1. TAB1 also promotes autophosphorylation and activation of the MAPK p38 alpha.

Long Name
TAK1-binding Protein
Entrez Gene IDs
10454 (Human); 66513 (Mouse); 315139 (Rat)
Alternate Names
MAP3K7IP1; MAP3K7IP13'-Tab1; MGC57664; mitogen-activated protein kinase kinase kinase 7 interacting protein 1; Mitogen-activated protein kinase kinase kinase 7-interacting protein 1; TAB1; TAK1-binding protein 1; TGF-beta activated kinase 1/MAP3K7 binding protein 1; TGF-beta-activated kinase 1 and MAP3K7-binding protein 1; TGF-beta-activated kinase 1-binding protein 1; transforming growth factor beta-activated kinase-binding protein 1

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Citations for Human/Mouse TAB1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. BRD4-specific PROTAC inhibits basal-like breast cancer partially through downregulating KLF5 expression
    Authors: Kong, Y;Lan, T;Wang, L;Gong, C;Lv, W;Zhang, H;Zhou, C;Sun, X;Liu, W;Huang, H;Weng, X;Cai, C;Peng, W;Zhang, M;Jiang, D;Yang, C;Liu, X;Rao, Y;Chen, C;
    Oncogene
    Species: Human
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  2. The Fbw7 tumor suppressor targets KLF5 for ubiquitin-mediated degradation and suppresses breast cell proliferation.
    Authors: Zhao D, Zheng HQ, Zhou Z
    Cancer Res., 2010-05-18;70(11):4728-38.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Chlamydia trachomatis antigen induces TLR4-TAB1-mediated inflammation, but not cell death, in maternal decidua cells
    Authors: Ortiz B, Driscoll A, Menon R et al.
    American journal of reproductive immunology (New York, N.Y. : 1989)

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