Human/Mouse SATB1 Antibody

Catalog #: MAB8075 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8075
MAB8075-SP

Detection of Human SATB1 by Western Blot.

2 Images

All SATB1 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse SATB1 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse SATB1 in ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 815427

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human SATB1
Met1-Ser260
Accession # Q01826

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human SATB1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human SATB1 Monoclonal Antibody (Catalog # MAB8075) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SATB1 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

View Larger

Detection of Human SATB1 by Simple Western^TM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.5 mg/mL. A specific band was detected for SATB1 at approximately 113 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse SATB1 Monoclonal Antibody (Catalog # MAB8075). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SATB1

SATB1 (special AT-rich sequence binding protein-1) is an approximately 103 kDa nuclear matrix-associated homeobox transcription factor that binds AT rich DNA sequences at scaffolding protein attachment and viral genome integration sites. It is a key factor integrating higher-order chromatin architecture with gene regulation. Specifically, it is involved in MHC class-I gene organization and chromatin organization in undifferentiated thymocytes. SATB1 is cleaved into 65-70 kDa and 25‑30 kDa fragments during apoptosis. Human SATB1 shares 99% amino acid sequence identity with mouse and rat SATB1.

Long Name

SATB Homeobox 1

Entrez Gene IDs

6304 (Human); 20230 (Mouse); 316164 (Rat)

Alternate Names

DNA-binding protein SATB1; SATB homeobox 1; SATB1; special AT-rich sequence binding protein 1 (binds to nuclearmatrix/scaffold-associating DNA's); Special AT-rich sequence-binding protein 1