Human/Mouse RUNX3/CBFA3 APC-conjugated Antibody Summary
Lys186-Tyr415
Accession # Q13761
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of RUNX3/CBFA3 in Human PBMC lymphocytes by Flow Cytometry. Human peripheral blood mononuclear cells (PBMC) lymphocytes either (A) untreated or (B) treated with PMA were stained with Mouse Anti-Human/Mouse RUNX3/CBFA3 APC-conjugated Monoclonal Antibody (Catalog # IC3765A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: RUNX3/CBFA3
RUNX3, also known as CBFA3, AML-2 or PEBP2-alpha C, is a member of the Runt domain family of nuclear transcriptional regulators. All of the RUNX proteins form dimers with CBF-beta. The Runt domain, amino acids (aa) 54-186, is required for DNA binding, while a pro/ser/thr-rich region (aa 191-415) transcriptionally activates target genes. Isoform 2 has an alternate 19 aa in place of the N-terminal 5 aa of isoform 1. The 415 aa Human RUNX3 shares 91% aa identity with mouse or rat RUNX3. RUNX3 is necessary for growth control of gastric epithelium, neurogenesis of dorsal root ganglia, and T cell differentiation. RUNX3 expression is frequently mutated in tumors and appears to be silenced by methylation.
Product Datasheets
Citations for Human/Mouse RUNX3/CBFA3 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Niche-specific MHC II and PD-L1 regulate CD4+CD8 alpha alpha + intraepithelial lymphocyte differentiation
Authors: Sookjin Moon, Yunji Park, Sumin Hyeon, Young-Min Kim, Ji-Hae Kim, Hyekang Kim et al.
Journal of Experimental Medicine
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Langerhans cells are generated by two distinct PU.1-dependent transcriptional networks.
Authors: Chopin M, Seillet C, Chevrier S, Wu L, Wang H, Morse H, Belz G, Nutt S
J Exp Med, 2013-11-18;210(13):2967-80.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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