Human/Mouse/Rat/Yeast Rad23 Antibody

Catalog #: AF4555 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4555
AF4555-SP

Detection of Human/Mouse/Rat/ Yeast Rad23 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat/Yeast Rad23 Antibody Summary

Species Reactivity

Human, Mouse, Rat, Yeast

Specificity

Detects human, mouse, rat, and yeast Rad23 protein in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant yeast Rad23
Accession # P32628

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat/ Yeast Rad23 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Raji human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, Nb2-11 rat lymphoma cell line, and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat/Yeast Rad23 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4555) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Rad23 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Rad23

Rad23 is an evolutionarily conserved protein that is important for nucleotide excision repair. All eukaryotic Rad23 proteins contain an amino-terminal UbL domain that bears a striking resemblance to ubiquitin. Rad23 has a proteolytic function in DNA repair via its interaction with the 26S proteosome through the UbL domain. The elimination of this domain causes sensitivity to ultraviolet radiation.

Entrez Gene IDs

5886 (Human)

Alternate Names

hHR23; hHR23A; HR23A; Rad23