Human/Mouse/Rat XPB Antibody

Catalog #: AF6349
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6349
AF6349-SP

Detection of Human, Mouse, and Rat XPB by Western Blot.

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All XPB Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat XPB Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat XPB in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human XPB
Met1-Lys375
Accession # P19447

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat XPB by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line, NRK rat normal kidney cell line, and RAW 264.7 mouse monocyte/macrophage cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat XPB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6349) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for XPB at approximately 86 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: XPB

XPB (Xeroderma pigmentosum group B complementing protein; also ERCC3 and TFIIH 89 kDa subunit) is an 86 kDa, nuclear member of the RAD25/XPB subfamily, helicase family of proteins. It is expressed in multiple cell types, including neurons, fibroblasts and keratinocytes. XPB is part of the 10 subunit TFIIH complex. As such, it appears to participate in both nucleotide excision repair (NER) and transcription initiation from RNA polymerase II promoters. During NER, XPB utilizes an intrinsic ATPase activity to resolve nucleotide excision and repair. Human XPB is 782 amino acids (aa) in length. It contains an NLS (aa 6‑18), multiple acidic-rich regions, an ATPase region (aa 327‑488) and one helicase domain (aa 542‑702). Over aa 1‑375, human XPB shares 93% aa identity with mouse XPB.

Long Name

Xeroderma Pigmentosum B

Entrez Gene IDs

2071 (Human); 13872 (Mouse); 291703 (Rat)

Alternate Names

Basic transcription factor 2 89 kDa subunit; BTF2 p89; BTF2; DNA excision repair protein ERCC-3; DNA repair protein complementing XP-B cells; EC 3.6.1; EC 3.6.4.12; ERCC3; excision repair cross-complementing rodent repair deficiency, complementationgroup 3 (xeroderma pigmentosum group B complementing); GTF2H; RAD25; TFIIH basal transcription factor complex 89 kDa subunit; TFIIH basal transcription factor complex helicase XPB subunit; TFIIH p89; TFIIH; Xeroderma pigmentosum group B-complementing protein; xeroderma pigmentosum, complementation group B; XPB; XPBC; XPBTFIIH 89 kDa subunit

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human/Mouse/Rat XPB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

OVO-like 1 regulates progenitor cell fate in human trophoblast development.
Authors: Renaud S, Chakraborty D, Mason C, Rumi M, Vivian J, Soares M
Proc Natl Acad Sci U S A, 2015-10-26;112(45):E6175-84.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: IHC, Western Blot