Human/Mouse/Rat UCH-L3 Antibody

Catalog #: AF6008 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6008
AF6008-SP

Detection of Human, Mouse, and Rat UCH‑L3 by Western Blot.

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Human/Mouse/Rat UCH-L3 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat UCH‑L3 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human UCH‑L3
Glu2-Ala230
Accession # P15374

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat UCH‑L3 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat UCH-L3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6008) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for UCH-L3 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2X.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: UCH-L3

UCH-L3 (Ubiquitin carboxyterminal hydrolase isozyme 3) is a 26‑28 kDa member of the peptidase C12 family of enzymes. It shows wide expression, being noted in adipocytes, renal duct epithelium, neurons, and striated muscle. UCH‑L3 cleaves both monomeric ubiquitin (Ub) from Ub‑protein conjugates, and a GGLRQ peptide from the C‑terminus of the Ub‑like protein NEDD‑8. Notably, UCH-L3 activity is muted in the presence of Ub interacting dimers. Human UCH-L3 is 230 amino acids (aa) in length. It is phosphorylated on Ser75 and Ser130, and contains two ubiquitin-binding sequences between aa 40‑57 and 178‑186. Four potential splice forms have been reported. Two show a four aa deletion between aa 15‑18, with one also containing a 14 aa substitution for aa 179‑230. Two others contain an alternative start site at Met37, one of which is also accompanied by a seven aa substitution for aa 143‑230. Full-length human UCH-L3 shares 98% aa identity with mouse and rat UCH‑L3.

Long Name

Ubiquitin C-terminal Hydrolase L3

Entrez Gene IDs

7347 (Human); 50933 (Mouse); 114094 (Rat); 100350137 (Rabbit)

Alternate Names

EC 3.4.19.12; ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase); ubiquitin carboxyl-terminal hydrolase isozyme L3; Ubiquitin Thioesterase L3; ubiquitin thiolesterase; UCHL3; UCH-L3