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Human/Mouse/Rat Total GAPDH DuoSet IC ELISA

Catalog #: DYC5718-2
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DYC5718-5
DYC5718-2
Ancillary Products Available
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
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Product Details
Citations (2)
FAQs
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Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human/Mouse/Rat Total GAPDH DuoSet IC ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4 hours 40 mins (after plate preparation)
Sample Volume Required
Cell lysates (100 µL)
Assay Range
156.0 - 10,000 pg/mL
Sufficient Materials
Kits available for two, five, or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure GAPDH in cell lysates. An immobilized capture antibody specific for GAPDH binds both phosphorylated and unphosphorylated GAPDH. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Available in 2, 5, and 15- (96-well) plate pack sizes
  • Economical alternative to Western blot

Kit Content

  • Capture Antibody
  • Conjugated Detection Antibody
  • Calibrated Immunoassay Standard or Control
  • Streptavidin-HRP

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Scientific Data

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Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: GAPDH

GAPDH (Glyceraldehyde-3-phosphate Dehydrogenase) is a relatively stable enzyme involved in the second phase of glycolysis. It is generally thought to be present at a constant level in cells, regardless (in most cases) of experimental treatment or technical procedure. For this reason, measurement of GAPDH is generally used as an internal control for experimental error.

Long Name:
Glyceraldehyde-3-phosphate Dehydrogenase
Entrez Gene IDs:
2597 (Human); 14433 (Mouse); 24383 (Rat)
Alternate Names:
aging-associated gene 9 protein; EC 1.2.1; EC 1.2.1.12; EC 2.6.99.-; G3PD; G3PDH; GAPD; GAPDH; glyceraldehyde 3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; MGC88685; Peptidyl-cysteine S-nitrosylase GAPDH

Citations for Human/Mouse/Rat Total GAPDH DuoSet IC ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Zinc Finger Protein St18 Protects against Septic Death by Inhibiting VEGF-A from Macrophages
    Authors: K Maruyama, H Kidoya, N Takemura, E Sugisawa, O Takeuchi, T Kondo, MMA Eid, H Tanaka, MM Martino, N Takakura, Y Takayama, S Akira, A Vandenbon, Y Kumagai
    Cell Rep, 2020-07-14;32(2):107906.
    Species: Mouse
    Sample Types: Cell Lysates
  2. Gene activation of SMN by selective disruption of lncRNA-mediated recruitment of PRC2 for the treatment of spinal muscular atrophy
    Authors: CJ Woo, VK Maier, R Davey, J Brennan, G Li, J Brothers, B Schwartz, S Gordo, A Kasper, TR Okamoto, HE Johansson, B Mandefro, D Sareen, P Bialek, BN Chau, B Bhat, D Bullough, J Barsoum
    Proc. Natl. Acad. Sci. U.S.A, 2017-02-13;114(8):E1509-E1518.
    Species: Human
    Sample Types: Cell Lysates

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