Human/Mouse/Rat HSP20/HSPB6 Antibody Summary
Met1-Lys160
Accession # O14558
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse/Rat HSP20/HSPB6 by Western Blot. Western blot shows lysates of human heart tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat HSP20/HSPB6 Monoclonal Antibody (Catalog # MAB4200) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HSP20/HSPB6 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human HSP20/HSPB6 by Simple WesternTM. Simple Western shows lysates of human heart tissue, loaded at 0.5 mg/ml. A specific band was detected for HSP20/HSPB6 at approximately 26 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat HSP20/HSPB6 Monoclonal Antibody (Catalog # MAB4200). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HSP20/HSPB6
Heat shock protein 20 kDa (HSP20), also known as heat shock protein, alpha-crystallin-related, B6 (HSPB6), is a 160 amino acid, 20 kDa member of the small heat shock protein family, which also includes HSP27, HSP25, and the alpha -crystallins. Heat shock proteins (HSPs) are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. HSPs are ubiquitously expressed in all organisms. Many HSPs are induced in response to various types of environmental stresses like heat, cold, and oxygen deprivation. Although HSP20 expression does not increase in response to heat stress, HSP20 does translocate from the cytosol to the nucleus during heat stress. The highest levels of HSP20 are found in skeletal, smooth and cardiac muscle. HSP20 may also be secreted into plasma. Human HSP20 shares 88% and 90% amino acid identity with mouse and rat HSP20, respectively.
Product Datasheets
Citation for Human/Mouse/Rat HSP20/HSPB6 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Small heat shock proteins operate as molecular chaperones in the mitochondrial intermembrane space
Authors: E Adriaensse, B Asselbergh, P Rivera-Mej, S Bervoets, L Vendredy, V De Winter, K Spaas, R de Rycke, G van Isterd, F Impens, T Langer, V Timmerman
Nature Cell Biology, 2023-01-23;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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