Human/Mouse/Rat/Hamster ACE-2 Antibody

Catalog # Availability Size / Price Qty
MAB3437
MAB3437-SP
Detection of Human ACE‑2 by Western Blot.
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Product Details
Citations (7)
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Human/Mouse/Rat/Hamster ACE-2 Antibody Summary

Species Reactivity
Human, Mouse, Rat, Hamster
Specificity
Detects mouse ACE-2 in direct ELISAs and human, mouse, rat and hamster ACE-2 in Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse ACE-1 is observed.
Source
Monoclonal Rat IgG2B Clone # 460502
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse ACE-2
Gln18-Thr740 (predicted)
Accession # Q8R0I0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
Human kidney and recombinant human ACE-2 protein, recombinant mouse ACE-2 protein, recombinant rat ACE-2 protein, and recombinant hamster ACE-2 protein

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Human ACE‑2 by Western Blot. Western blot shows lysates of human kidney. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human/Mouse/Rat/Hamster ACE‑2 Monoclonal Antibody (Catalog # MAB3437) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band was detected for ACE‑2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Western Blot View Larger

Detection of Human, Mouse, Rat, and Hamster ACE‑2 by Western Blot. Western blot shows recombinant human ACE-2 protein, recombinant mouse ACE-2 protein, recombinant rat ACE-2 protein, and recombinant hamster ACE-2 protein. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human/Mouse/Rat/Hamster ACE‑2 Monoclonal Antibody (Catalog # MAB3437) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band was detected for ACE‑2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ACE-2

ACE-2, also called ACEH (ACE homologue), is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE (1). Mouse ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of mouse somatic ACE. The predicted mouse ACE-2 protein sequence consists of 798 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 mRNA is found at high levels in testis, kidney and heart and at moderate levels in colon, small intestine and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3). In addition, ACE-2 is a key SARS-CoV Spike protein receptor in vivo and has a critical function in acute lung injury (4, 5).

References
  1. Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
  2. Crackower, M.A. et al. (2002) Nature 417:822.
  3. Huang, L. et al. (2003) J. Biol. Chem. 278:15532.
  4. Kuba, K. et al. (2005) Nature Med. 11:875.
  5. Ima, Y. et al. (2005) Nature 436:112.
Long Name
Angiotensin I Converting Enzyme 2
Entrez Gene IDs
59272 (Human); 70008 (Mouse); 302668 (Rat); 100144303 (Porcine); 480847 (Canine); 418623 (Chicken); 102130864 (Cynomolgus Monkey); 554349 (Feline); 101673097 (Ferret); 101823817 (Hamster); 108390919 (Malayan Pangolin)
Alternate Names
ACE2; ACE-2; ACEH; ACEHangiotensin I converting enzyme 2; ACE-related carboxypeptidase; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin-converting enzyme 2; Angiotensin-converting enzyme homolog; DKFZp434A014; EC 3.4.17; EC 3.4.17.23; Metalloprotease MPROT15

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Citations for Human/Mouse/Rat/Hamster ACE-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Age-dependent regulation of SARS-CoV-2 cell entry genes and cell death programs correlates with COVID-19 disease severity
    Authors: Zintis Inde, Clarence Yapp, Gaurav N. Joshi, Johan Spetz, Cameron Fraser, Brian Deskin et al.
    bioRxiv
  2. Gut microbiota-derived metabolites confer protection against SARS-CoV-2 infection
    Authors: Julia A. Brown, Katherine Z. Sanidad, Serena Lucotti, Carolin M. Lieber, Robert M. Cox, Aparna Ananthanarayanan et al.
    Gut Microbes
  3. Characterization of SARS-CoV-2 Spike mutations important for infection of mice and escape from human immune sera
    Authors: R Rathnasing, S Jangra, C Ye, A Cupic, G Singh, C Martínez-R, LCF Mulder, T Kehrer, S Yildiz, A Choi, ST Yeung, I Mena, V Gillespie, J De Vrieze, S Aslam, D Stadlbauer, DA Meekins, CD McDowell, V Balaraman, MJ Corley, JA Richt, BG De Geest, L Miorin, PVI study, F Krammer, L Martinez-S, V Simon, A García-Sas, M Schotsaert
    Nature Communications, 2022-07-07;13(1):3921.
    Species: African Green Monkey
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Interferon-alpha or -beta facilitates SARS-CoV-2 pulmonary vascular infection by inducing ACE2
    Authors: Timothy Klouda, Yuan Hao, Hyunbum Kim, Jiwon Kim, Judith Olejnik, Adam J. Hume et al.
    Angiogenesis
  5. SARS-CoV-2 Causes Lung Infection without Severe Disease in Human ACE2 Knock-In Mice
    Authors: Emma S. Winkler, Rita E. Chen, Fahmida Alam, Soner Yildiz, James Brett Case, Melissa B. Uccellini et al.
    Journal of Virology
  6. The N501Y mutation in SARS-CoV-2 spike leads to morbidity in obese and aged mice and is neutralized by convalescent and post-vaccination human sera
    Authors: R Rathnasing, S Jangra, A Cupic, C Martínez-R, LCF Mulder, T Kehrer, S Yildiz, A Choi, I Mena, J De Vrieze, S Aslam, D Stadlbauer, DA Meekins, CD McDowell, V Balaraman, JA Richt, BG De Geest, L Miorin, F Krammer, V Simon, A García-Sas, M Schotsaert
    medRxiv : the preprint server for health sciences, 2021-01-20;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Angiotensin-converting enzyme 2 protects from lethal avian influenza A H5N1 infections.
    Authors: Zou, Zhen, Yan, Yiwu, Shu, Yuelong, Gao, Rongbao, Sun, Yang, Li, Xiao, Ju, Xiangwu, Liang, Zhu, Liu, Qiang, Zhao, Yan, Guo, Feng, Bai, Tian, Han, Zongshen, Zhu, Jindong, Zhou, Huandi, Huang, Fengming, Li, Chang, Lu, Huijun, Li, Ning, Li, Dangshen, Jin, Ningyi, Penninger, Josef M, Jiang, Chengyu
    Nat Commun, 2014-05-06;5(0):3594.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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