Human/Mouse/Rat GULP1/CED-6 Antibody

Catalog #: AF7978 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7978
AF7978-SP

Detection of Human, Mouse, and Rat GULP1/CED-6 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat GULP1/CED-6 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat GULP1/CED-6 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat GULP1/CED-6 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, MEF mouse embryonic feeder cells, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat GULP1/CED-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7978) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GULP1/CED-6 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: GULP1/CED-6

GULP1 (also PTB domain-containing engulfment adaptor protein 1 and Cell death protein 6 homolog/CED6) is a 36-40 kDa, cytosolic member of the ced-6 family of proteins. It is expressed by multiple cell types, including macrophages, neurons and sinusoidal endothelial cells, and is believed to play a key role in phaogcytosis, particularly involving apoptotic cells. Failure to remove apoptotic cells is suggested to contribute to chronic inflammatory conditions. GULP1 is involved with a MEGF10:GULF:stabilin-1:stabilin-2 internalization pathway. Possibly following endosome formation, it binds to transmembrane activated stabilins and/or LRP1 as a homodimer, and serves as an intermediate for signal transduction. GULP1 also binds to APP, facilitating its proteolysis and the generation of A beta. Human GULP1 is 304 amino acids (aa) in length. It contains one PTB domain (aa 17-154) that interacts with transmembrane receptors, a Leu-zipper (aa 163-193) that mediates dimerization, and a Pro-rich region (aa 238-271) that is likely involved with signal transduction. There are three splice variants. One shows a deletion of aa 31-133, a second contains a 34 aa substitution for aa 134-304, and a third contains a 10 aa substitution for aa 282-304. Over aa 1-92, human GULP1 shares 99% aa sequence identity with mouse GULP1.

Entrez Gene IDs

51454 (Human); 70676 (Mouse); 314543 (Rat)

Alternate Names

CED6; CED6GULPCED-6; Cell death protein 6 homolog; engulfment adapter protein; FLJ31156; GULP, engulfment adaptor PTB domain containing 1; GULP1; Protein GULP; PTB domain adapter protein CED-6; PTB domain adaptor protein CED-6; PTB domain-containing engulfment adapter protein 1