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Human/Mouse/Rat GRIN2A/NMDAR2A Antibody

Catalog #: PPS012
6 Citations Datasheet / COA / SDS
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PPS012
Detection of GRIN2A/NMDAR2A by Western Blot.
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Citations (6)
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Human/Mouse/Rat GRIN2A/NMDAR2A Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Specific for the ~180 kDa NR2A subunit of the NMDA Receptor in Western blots of most brain regions. No reactivity towards the NR2B and NR2C subunits. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used to generate the antibody.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Fusion protein from the C-terminus of the NR2A subunit of rat NMDA Receptor
Formulation
Lyophilized from 5 mM ammonium bicarbonate
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1:1000 dilution
See below
Immunohistochemistry
1:1000 to 1:2000 dilution
Frozen sections; unpublished observations
Immunoprecipitation
3 μL per 200 μg lysate
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of GRIN2A/NMDAR2A antibody by Western Blot. View Larger

Detection of GRIN2A/NMDAR2A by Western Blot. Western blot of 10 μg of rat brain hippocampus lysate showing specific immunolabeling of the ~180 kDa NR2A subunit of the NMDA Receptor. The labeling by the antibody was blocked by the N-terminal NR2A-subunit of the NMDA Receptor fusion protein (not shown).

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
This antibody should be reconstituted in 50 μL phosphate buffered saline (137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use.
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Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
After reconstitution the antibody should be aliquoted and stored at ≤ -20° C. This product is stable at ≤- 20° C for at least 1 year.

Background: GRIN2A/NMDAR2A

NMDA (N-Methyl D-Aspartate) receptors are members of the glutamate receptor family of ligand-gated ion channels. The functional NMDA receptor (NMDAR) is a 650-850 kDa heteromultimer of at least two NR2 (NMDAR2) subunits and two NR1 subunits. NR2 subunits determine overall NMDAR characteristics such as conductance and Mg++ sensitivity. In addition, NR2 subunits mediate NMDAR clustering and synaptic localization through cytoplasmic interaction with PSD-95/SAP90 family members. Upon glutamate binding to NR2, and glycine binding to NR1, the NMDA channel is opened, allowing calcium and sodium influx into the cell. There are four genes, which code for NR2 subunits (NR2A-D). The two (or three) NR2 subunits making up the NMDAR may be homodimers or heterodimers. The 2A form generates the highest conductance NMDAR. Rat NR2A is a 180 kDa, 1464 amino acid (aa), three transmembrane (TM) glycoprotein that contains a 533 aa extracellular domain (ECD) and a 627 aa cytoplasmic region. The molecule is described as 4-TM. However, the second-TM segment is only partial (or reenterant), and this makes the C-terminus intracellular. The loop connecting TM segments 3 and 4, plus the N-terminal ECD constitutes the glutamate binding site. Rat NR2 is 99% and 95% aa identical to mouse and human NR2, respectively.

Long Name
Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate, Subunit 2A
Entrez Gene IDs
2903 (Human); 14811 (Mouse); 24409 (Rat)
Alternate Names
EPND; FESD; GluN1; GluN2A; GRIN2A; LKS; MRD8; NDHMSD; NDHMSR; NMDA R, NR2A Subunit; NMDA1; NMDAR1; NMDAR2A; NMD-R1; NR1; NR2A

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Citations for Human/Mouse/Rat GRIN2A/NMDAR2A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Transmembrane protein 108 is required for glutamatergic transmission in dentate gyrus
    Authors: HF Jiao, XD Sun, R Bates, L Xiong, L Zhang, F Liu, L Li, HS Zhang, SQ Wang, MT Xiong, M Patel, AM Stranahan, WC Xiong, BM Li, L Mei
    Proc. Natl. Acad. Sci. U.S.A, 2017-01-17;114(5):1177-1182.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Lrp4 in astrocytes modulates glutamatergic transmission
    Authors: Xiang-Dong Sun
    Nat Neurosci, 2016-06-13;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Altered hippocampal plasticity by prenatal kynurenine administration, kynurenine-3-monoxygenase (KMO) deletion or galantamine
    Authors: C.M. Forrest, K. McNair, M. Pisar, O.S. Khalil, L.G. Darlington, T.W. Stone
    Neuroscience
  4. Mutant disrupted-in-schizophrenia 1 in astrocytes: Focus on glutamate metabolism
    Authors: Sofya Abazyan, Eun Ju Yang, Bagrat Abazyan, Meng Xia, Chunxia Yang, Camilo Rojas et al.
    Journal of Neuroscience Research
  5. Changes in synaptic transmission and protein expression in the brains of adult offspring after prenatal inhibition of the kynurenine pathway
    Authors: C.M. Forrest, O.S. Khalil, M. Pisar, K. McNair, E. Kornisiuk, M. Snitcofsky et al.
    Neuroscience
  6. Prenatal activation of Toll-like receptors-3 by administration of the viral mimetic poly(I:C) changes synaptic proteins, N-methyl-D-aspartate receptors and neurogenesis markers in offspring.
    Authors: Forrest, Caroline, Khalil, Omari S, Pisar, Mazura, Smith, Robert A, Darlington, Lynda Ga, Stone, Trevor W
    Mol Brain, 2012-06-09;5(0):22.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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