Human/Mouse/Rat Glyoxalase II Antibody Summary
Met49-Ile254
Accession # Q16775
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of Human, Mouse, and Rat Glyoxalase II by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, HEK293 human embryonic kidney cell line, human liver, human kidney, mouse liver, and rat liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Glyoxalase II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5944) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Glyoxalase II at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Glyoxalase II
Glyoxalase II (GLO2; also hydroxyacylglutathione hydrolase 2 and Glx II) is a monomeric, cytosolic 29 kDa member of the Glyoxylase II family, metallo-beta -lactamase superfamily of enzymes. It is expressed in liver and kidney, and converts GLO1-generated lactoylglutathione into lactate and GSH. It is up‑regulated by p63 and p73, and thus serves as a p53-associated survival factor. Human GLO2 is 260 amino acids (aa) in length. It binds two atoms of Zn and contains one substrate binding region (aa 143‑145 plus 249‑252). The 29 kDa form represents 80‑90% of cellular GLO2. There is also a 32‑33 kDa, 308 aa mitochondrial isoform that arises via the use of an alternative start site. Its function is unclear, given that mitochondria do not contain GLO1. There is an additional potential isoform that shows an insertion of nine aa after Thr167. Human GLO2 shares approximately 90% aa identity with mouse GLO2.
Product Datasheets
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human/Mouse/Rat Glyoxalase II Antibody
There are currently no reviews for this product. Be the first to review Human/Mouse/Rat Glyoxalase II Antibody and earn rewards!
Have you used Human/Mouse/Rat Glyoxalase II Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image