Home / GAPDH / Human/Mouse/Rat GAPDH Antibody

Human/Mouse/Rat GAPDH Antibody

Catalog #: 2275-PC-100
107 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
2275-PC-100
Detection of Human, Mouse, and Rat GAPDH/G3PDH by Western Blot.
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Citations (107)
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Human/Mouse/Rat GAPDH Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat GAPDH/G3PDH in Western blots.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Synthetic peptide corresponding to a portion of the human GAPDH/G3PDH sequence
Formulation
Supplied as a solution containg PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
1-5 µg/mL
Exosome Standards (LNCap) (Catalog # NBP3-11687), Exosome Standards (HT-29) (Catalog # NBP3-11685), Jurkat human acute T cell leukemia cell line, C2C12 mouse myoblast cell line, and C6 rat glioma cell line
Immunoprecipitation
1:1000-1:5000 dilution
L292 human fibrosarcoma cell line, WEHI 7.1 mouse T cell lymphoma cell line, and INT 407 human intestinal epithelial cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat GAPDH/G3PDH antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat GAPDH/G3PDH by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line, A20 mouse B cell lymphoma cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.1 µg/mL Rabbit Anti-Human/Mouse/Rat GAPDH Polyclonal Antibody (Catalog # 2275-PC) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for GAPDH at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western View Larger

Detection of Human GAPDH by Simple WesternTM. Simple Western shows lysates of Exosome Standards (LNCaP) (NBP3-11687), Exosome Standards (HT‑29) (NBP3-11685) and Jurkat human acute T cell leukemia cell line, loaded at 0.5 mg/ml. A specific band was detected for GAPDH at approximately 41 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat GAPDH Antigen Affinity-purified Polyclonal Antibody (Catalog # 2275-PC-100). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Simple Western View Larger

Detection of Human, Mouse and Rat GAPDH by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, C2C12 mouse myoblast cell line, and C6 rat glioma cell line, loaded at 0.2 mg/mL. A specific band was detected for GAPDH at approximately 43 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human/Mouse/Rat GAPDH Antigen Affinity-purified Polyclonal Antibody (Catalog # 2275-PC-100). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot Detection of Mouse GAPDH by Western Blot View Larger

Detection of Mouse GAPDH by Western Blot Higher levels of CD4 are found in macrophages expressing LCK.A) Schematic diagram of self-inactivating lentiviral construct used to express wild-type Lck and puroR (LCKWT P). A similar construct was used to express a kinase inactive form of Lck and puroR (LCKINACTIVE P) or to express puroR only (‘control P’). B) Detection of protein expression of LCK and CD4. Control and transgenic PSC-macrophages lysates were analysed by western blotting using anti-LCK and anti-CD4 antibodies. The loading control GAPDH was detected using anti-GAPDH antibody. C) Protein levels were measured with Odyssey software (Li-COR) and CD4 expression was normalised to GAPDH expression. Symbols represent normalised CD4 expression, relative to the PSC-macrophages control group, of two independent experiments. D) Detection of surface CD4 and total LCK expression. Representative two-colour immunofluorescence (dot plot) analysis is shown. Gates were determined by using the two relevant isotype control antibodies. Quantification of total LCK expression (E) and surface CD4 expression (F), expressed as the ratio of the geometric mean fluorescence intensity (MFI) over the isotype control ±SEM of independent experiments (n = 7). G) Detergent resistance of CD4, expressed as mean Flow Cytometric Detergent Resistance (FCDR) index of CD4 (n = 4) in PSC-macrophages, calculated as described in materials and methods. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0086071), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse Human/Mouse/Rat GAPDH Antibody by Western Blot View Larger

Detection of Mouse Human/Mouse/Rat GAPDH Antibody by Western Blot Higher levels of CD4 are found in macrophages expressing LCK.A) Schematic diagram of self-inactivating lentiviral construct used to express wild-type Lck and puroR (LCKWT P). A similar construct was used to express a kinase inactive form of Lck and puroR (LCKINACTIVE P) or to express puroR only (‘control P’). B) Detection of protein expression of LCK and CD4. Control and transgenic PSC-macrophages lysates were analysed by western blotting using anti-LCK and anti-CD4 antibodies. The loading control GAPDH was detected using anti-GAPDH antibody. C) Protein levels were measured with Odyssey software (Li-COR) and CD4 expression was normalised to GAPDH expression. Symbols represent normalised CD4 expression, relative to the PSC-macrophages control group, of two independent experiments. D) Detection of surface CD4 and total LCK expression. Representative two-colour immunofluorescence (dot plot) analysis is shown. Gates were determined by using the two relevant isotype control antibodies. Quantification of total LCK expression (E) and surface CD4 expression (F), expressed as the ratio of the geometric mean fluorescence intensity (MFI) over the isotype control ±SEM of independent experiments (n = 7). G) Detergent resistance of CD4, expressed as mean Flow Cytometric Detergent Resistance (FCDR) index of CD4 (n = 4) in PSC-macrophages, calculated as described in materials and methods. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24465876), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse Human/Mouse/Rat GAPDH Antibody by Western Blot View Larger

Detection of Mouse Human/Mouse/Rat GAPDH Antibody by Western Blot CD4 knock-down in genetically modified stem cell-derived macrophages. C) Detection of protein expression of CD4. Control and transgenic PSC-macrophages lysates were analysed by western blotting using anti-CD4 antibodies. GAPDH, a loading control, was detected using anti-GAPDH antibody. Representative blot is shown. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24465876), licensed under a CC-BY license. Not internally tested by R&D Systems.

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage


Background: GAPDH

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a 36‑40 kDa member of the GAPDH family of enzymes. It is a widely expressed heterotetramer that is found in both the nucleus and cytoplasm. Although GAPDH was initially identified as a glycolytic enzyme that converted G3P into 1,3 diphosphoglycerate, it is now recognized to participate in no less than endocytosis, membrane fusion, vesicular secretory transport, DNA replication and repair, and apoptosis. Human GAPDH is 335 amino acids (aa) in length and contains two NAD binding sites (Asp35 and Asn316) with a catalytic region between aa 151‑155. GAPDH contains more than 19 posttranslational modifications, including methylation, deamidation and phosphorylation. One splice variant shows a 10 aa substitution for aa 319‑335. Over amino acid 1‑150, human GAPDH shares 92% aa identity with mouse GAPDH.

Long Name
Glyceraldehyde-3-phosphate Dehydrogenase
Entrez Gene IDs
2597 (Human); 14433 (Mouse); 24383 (Rat)
Alternate Names
aging-associated gene 9 protein; EC 1.2.1; EC 1.2.1.12; EC 2.6.99.-; G3PD; G3PDH; GAPD; GAPDH; glyceraldehyde 3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; MGC88685; Peptidyl-cysteine S-nitrosylase GAPDH

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Citations for Human/Mouse/Rat GAPDH Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

107 Citations: Showing 1 - 10
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  1. Rational engineering of an improved adenosine deaminase 2 enzyme for weaponizing T-cell therapies
    Authors: J.R. Cox, M. Jennings, C. Lenahan, M. Manion, S. Courville, J. Blazeck
    Immuno-Oncology and Technology
  2. Compromised NLRP3 and AIM2 inflammasome function in autoimmune NZB/W F1 mouse macrophages
    Authors: SJ Thygesen, KE Takizawa, AAB Robertson, DP Sester, KJ Stacey
    Immunol. Cell Biol., 2018-08-19;0(0):.
  3. A variant in human AIOLOS impairs adaptive immunity by interfering with IKAROS
    Authors: M Yamashita, HS Kuehn, K Okuyama, S Okada, Y Inoue, N Mitsuiki, K Imai, M Takagi, H Kanegane, M Takeuchi, N Shimojo, M Tsumura, AK Padhi, KYJ Zhang, B Boisson, JL Casanova, O Ohara, SD Rosenzweig, I Taniuchi, T Morio
    Nature Immunology, 2021-06-21;22(7):893-903.
  4. Y-box binding protein 1/cyclin A1 axis specifically promotes cell cycle progression at G2/M phase in ovarian cancer
    Authors: Murakami, Y;Katsuchi, D;Matsumoto, T;Kanazawa, K;Shibata, T;Kawahara, A;Akiba, J;Yanaihara, N;Okamoto, A;Itamochi, H;Sugiyama, T;Terada, A;Nishio, S;Tsuda, N;Kato, K;Ono, M;Kuwano, M;
    Scientific reports
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot Control
  5. SMARCB1 Gene Therapy Using a Novel Tumor-Targeted Nanomedicine Enhances Anti-Cancer Efficacy in a Mouse Model of Atypical Teratoid Rhabdoid Tumors
    Authors: Kim, SS;Moghe, M;Rait, A;Donaldson, K;Harford, JB;Chang, EH;
    International journal of nanomedicine
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Deficiency of endothelial sirtuin1 in mice stimulates skeletal muscle insulin sensitivity by modifying the secretome
    Authors: Li, Q;Zhang, Q;Kim, YR;Gaddam, RR;Jacobs, JS;Bachschmid, MM;Younis, T;Zhu, Z;Zingman, L;London, B;Rauckhorst, AJ;Taylor, EB;Norris, AW;Vikram, A;Irani, K;
    Nature communications
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Identification of a novel enhancer essential for Satb1 expression in TH2 cells and activated ILC2s
    Authors: Aneela Nomura, Tetsuro Kobayashi, Wooseok Seo, Michiko Ohno-Oishi, Kiyokazu Kakugawa, Sawako Muroi et al.
    Life Science Alliance
  8. v-Src delocalizes Aurora B by suppressing Aurora B kinase activity during monopolar cytokinesis
    Authors: Ota, R;Watanabe, T;Wazawa, Y;Kuwajima, H;Honda, T;Soeda, S;Saito, Y;Yuki, R;Fukumoto, Y;Yamaguchi, N;Yamaguchi, N;Nakayama, Y;
    Cellular signalling
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Novel Bruton's tyrosine kinase inhibitor TAS5315 suppresses the progression of inflammation and joint destruction in rodent collagen-induced arthritis
    Authors: D Akasaka, S Iguchi, R Kaneko, Y Yoshiga, D Kajiwara, Y Nakachi, N Noma, K Tanaka, A Shimizu, F Hosoi
    PLoS ONE, 2023-02-23;18(2):e0282117.
    Species: Human
    Sample Types: Whole Cells
    Applications: Positive Control
  10. Selective Eradication of Colon Cancer Cells Harboring PI3K and/or MAPK Pathway Mutations in 3D Culture by Combined PI3K/AKT/mTOR Pathway and MEK Inhibition
    Authors: VS Atanasova, A Riedl, M Strobl, J Flandorfer, D Unterleuth, C Weindorfer, P Neuhold, S Stang, M Hengstschl, M Bergmann, H Dolznig
    International Journal of Molecular Sciences, 2023-01-14;24(2):.
    Species: Human
    Sample Types: Cell culture supernate
    Applications: Western Blot
  11. Platelet-derived growth factor D expression in adrenal cells is modulated by corticosteroids: putative role in adrenal suppression
    Authors: Christopher M. Parry, Li F. Chan, Daniel F. Carr, Daniel B. Hawcutt
    Pediatric Research
  12. Vitamin C enhances NF-kappaB-driven epigenomic reprogramming and boosts the immunogenic properties of dendritic cells
    Authors: O Morante-Pa, G Godoy-Tena, J Calafell-S, L Ciudad, EM Martínez-C, JL Sardina, E Ballestar
    Nucleic Acids Research, 2022-10-28;50(19):10981-10994.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Nanomedicine-Based Gene Delivery for a Truncated Tumor Suppressor RB94 Promotes Lung Cancer Immunity
    Authors: SS Kim, C Doherty, M Moghe, A Rait, KF Pirollo, JB Harford, EH Chang
    Cancers, 2022-10-18;14(20):.
    Species: Xenograft
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Retinal Pigment Epithelium-Secreted VEGF-A Induces Alpha-2-Macroglobulin Expression in Endothelial Cells
    Authors: GL Lehmann, M Ginsberg, DJ Nolan, C Rodríguez, J Martínez-G, S Zeng, AP Voigt, RF Mullins, S Rafii, E Rodriguez-, I Benedicto
    Cells, 2022-09-24;11(19):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. Senescent Human Pancreatic Stellate Cells Secrete CXCR2 Agonist CXCLs to Promote Proliferation and Migration of Human Pancreatic Cancer AsPC-1 and MIAPaCa-2 Cell Lines
    Authors: T Takikawa, S Hamada, R Matsumoto, Y Tanaka, F Kataoka, A Sasaki, A Masamune
    International Journal of Molecular Sciences, 2022-08-17;23(16):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. The conserved C-terminal residues of FAM83H are required for the recruitment of casein kinase 1 to the keratin cytoskeleton
    Authors: T Kuga, N Inoue, K Sometani, S Murataka, M Saraya, R Sugita, T Mikami, Y Takeda, M Taniguchi, K Nishida, N Yamagishi
    Scientific Reports, 2022-07-12;12(1):11819.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. NF-kappaB-inducing kinase (NIK) is activated in pancreatic beta-cells but does not contribute to the development of diabetes
    Authors: P Xiao, T Takiishi, NM Violato, G Licata, F Dotta, G Sebastiani, L Marselli, SP Singh, M Sze, G Van Loo, E Dejardin, EN Gurzov, AK Cardozo
    Cell Death & Disease, 2022-05-19;13(5):476.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. Rapid lamellipodial responses by neighbor cells drive epithelial sealing in response to pyroptotic cell death
    Authors: A Bonfim-Mel, I Noordstra, S Gupta, AH Chan, MJK Jones, K Schroder, AS Yap
    Cell Reports, 2022-02-01;38(5):110316.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  19. Effects of 2-Methoxyestradiol, a Main Metabolite of Estradiol on Hepatic ABCA1 Expression in HepG2 Cells
    Authors: T Ibata, J Lyu, H Imachi, K Fukunaga, S Sato, T Kobayashi, T Saheki, T Yoshimura, K Murao
    Nutrients, 2022-01-11;14(2):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  20. Coordinated glucocorticoid receptor and MAFB action induces tolerogenesis and epigenome remodeling in dendritic cells
    Authors: O Morante-Pa, L Ciudad, R Micheroli, C de la Call, T Li, G Barbisan, M Houtman, SG Edalat, M Frank-Bert, C Ospelt, E Ballestar
    Nucleic Acids Research, 2022-01-11;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. Bone mesenchymal stem cells-derived miR-223-3p-containing exosomes ameliorate lipopolysaccharide-induced acute uterine injury via interacting with endothelial progenitor cells
    Authors: Yana Liu, Shihong Zhang, Zhiwei Xue, Xiaoxia Zhou, Lin Tong, Jiachen Liao et al.
    Bioengineered
  22. T and B cell abnormalities, pneumocystis pneumonia, and chronic lymphocytic leukemia associated with an AIOLOS defect in patients
    Authors: Hye Sun Kuehn, Jingjie Chang, Motoi Yamashita, Julie E. Niemela, Chengcheng Zou, Kazuki Okuyama et al.
    Journal of Experimental Medicine
  23. TGF-beta -induced alpha -SMA expression is mediated by C/EBP beta acetylation in human alveolar epithelial cells
    Authors: Hui Ding, Jinjun Chen, Jingping Qin, Ruhua Chen, Zili Yi
    Molecular Medicine
  24. The RNA helicase Ddx21 controls Vegfc-driven developmental lymphangiogenesis by balancing endothelial cell ribosome biogenesis and p53 function
    Authors: K Koltowska, KS Okuda, M Gloger, M Rondon-Gal, E Mason, J Xuan, S Dudczig, H Chen, H Arnold, R Skoczylas, NI Bower, S Paterson, AK Lagendijk, GJ Baillie, I Leshchiner, C Simons, KA Smith, W Goessling, JK Heath, RB Pearson, E Sanij, S Schulte-Me, BM Hogan
    Nature Cell Biology, 2021-11-08;23(11):1136-1147.
    Species: Human
    Sample Types:
  25. A spoonful of L-fucose-an efficient therapy for GFUS-CDG, a new glycosylation disorder
    Authors: RG Feichtinge, A Hüllen, A Koller, D Kotzot, V Grote, E Rapp, P Hofbauer, K Brugger, C Thiel, JA Mayr, SB Wortmann
    Embo Molecular Medicine, 2021-09-01;13(9):e14332.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  26. Progesterone depletion results in Lamin B1 loss and induction of cell death in mouse trophoblast giant cells
    Authors: H Morimoto, M Ueno, H Tanabe, T Kono, H Ogawa
    PLoS ONE, 2021-07-14;16(7):e0254674.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  27. ABT?737, a Bcl?2 family inhibitor, has a synergistic effect with apoptosis by inducing urothelial carcinoma cell necroptosis
    Authors: R Cheng, X Liu, Z Wang, K Tang
    Molecular Medicine Reports, 2021-03-31;23(6):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  28. EBF1 drives hallmark B cell gene expression by enabling the interaction of PAX5 with the MLL H3K4 methyltransferase complex
    Authors: CE Bullerwell, PP Robichaud, PML Deprez, AP Joy, G Wajnberg, D D'Souza, S Chacko, S Fournier, N Crapoulet, DA Barnett, SM Lewis, RJ Ouellette
    Scientific Reports, 2021-01-15;11(1):1537.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  29. Activation of PKC supports the anticancer activity of tigilanol tiglate and related epoxytiglianes
    Authors: JK Cullen, GM Boyle, PY Yap, S Elmlinger, JL Simmons, N Broit, J Johns, B Ferguson, LA Maslovskay, AI Savchenko, PM Mirzayans, A Porzelle, PV Bernhardt, VA Gordon, PW Reddell, A Pagani, G Appendino, PG Parsons, CM Williams
    Scientific Reports, 2021-01-08;11(1):207.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  30. Src kinases relax adherens junctions between the neighbors of apoptotic cells to permit apical extrusion
    Authors: Jessica L. Teo, Vanesa M. Tomatis, Luke Coburn, Anne K. Lagendijk, Irin-Maya Schouwenaar, Srikanth Budnar et al.
    Molecular Biology of the Cell
  31. Aprotinin Inhibits SARS-CoV-2 Replication
    Authors: D Bojkova, M Bechtel, KM McLaughlin, JE McGreig, K Klann, C Bellinghau, G Rohde, D Jonigk, P Braubach, S Ciesek, C Münch, MN Wass, M Michaelis, J Cinatl
    Cells, 2020-10-30;9(11):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  32. The DNA methyltransferase DNMT3A contributes to autophagy long-term memory
    Authors: P González-R, M Cheray, J Füllgrabe, M Salli, P Engskog-Vl, L Keane, V Cunha, A Lupa, W Li, Q Ma, K Dreij, MG Rosenfeld, B Joseph
    Autophagy, 2020-09-14;0(0):1-19.
    Species: Human, Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  33. Depletion of Csk preferentially reduces the protein level of LynA in a Cbl-dependent manner in cancer cells
    Authors: T Kuga, Y Yamane, S Hayashi, M Taniguchi, N Yamaguchi, N Yamagishi
    Sci Rep, 2020-05-06;10(1):7621.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  34. Nrf2 activation protects against lithium-induced nephrogenic diabetes insipidus
    Authors: S Jobbagy, DA Vitturi, SR Salvatore, MF Pires, P Rowart, DR Emlet, M Ross, S Hahn, C St Croix, SG Wendell, AR Subramanya, AC Straub, RJ Tan, FJ Schopfer
    JCI Insight, 2020-01-16;5(1):.
    Species: Mouse
    Sample Types: Tissue Lysate
    Applications: Western Blot
  35. Receptor Heterodimerization and Co-Receptor Engagement in TLR2 Activation Induced by MIC1 and MIC4 from Toxoplasma gondii
    Authors: F Costa Mend, C Duque Lope, R Ricci-Azev, A Sardinha-S, C Figueiredo, AC Paiva Aleg, L L Nohara, A B Carneiro, A Panunto-Ca, I C Almeida, MC Roque-Barr
    Int J Mol Sci, 2019-10-10;20(20):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  36. Blockade of leukemia inhibitory factor as a therapeutic approach to KRAS driven pancreatic cancer
    Authors: MT Wang, N Fer, J Galeas, EA Collisson, SE Kim, J Sharib, F McCormick
    Nat Commun, 2019-07-11;10(1):3055.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  37. Humanization of the entire murine Mapt gene provides a murine model of pathological human tau propagation
    Authors: T Saito, N Mihira, Y Matsuba, H Sasaguri, S Hashimoto, S Narasimhan, B Zhang, S Murayama, M Higuchi, VMY Lee, JQ Trojanowsk, TC Saido
    J. Biol. Chem., 2019-07-04;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  38. Anticancer effects of a non-narcotic opium alkaloid medicine, papaverine, in human glioblastoma cells
    Authors: M Inada, M Shindo, K Kobayashi, A Sato, Y Yamamoto, Y Akasaki, K Ichimura, SI Tanuma
    PLoS ONE, 2019-05-17;14(5):e0216358.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  39. MCC950 directly targets the NLRP3 ATP-hydrolysis motif for inflammasome inhibition
    Authors: RC Coll, JR Hill, CJ Day, A Zamoshniko, D Boucher, NL Massey, JL Chitty, JA Fraser, MP Jennings, AAB Robertson, K Schroder
    Nat. Chem. Biol., 2019-05-13;15(6):556-559.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  40. Propranolol regulates ERK1/2 signaling pathway and promotes chronic wound healing in diabetic rats
    Authors: X Chang, S Li, XD Xue, F Chang
    Eur Rev Med Pharmacol Sci, 2019-05-01;23(10):4498-4506.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  41. Single-Cell RNA-Sequencing-Based CRISPRi Screening Resolves Molecular Drivers of Early Human Endoderm Development
    Authors: RMJ Genga, EM Kernfeld, KM Parsi, TJ Parsons, MJ Ziller, R Maehr
    Cell Rep, 2019-04-16;27(3):708-718.e10.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  42. Coenzyme Q10 protects against burn-induced mitochondrial dysfunction and impaired insulin signaling in mouse skeletal muscle
    Authors: H Nakazawa, K Ikeda, S Shinozaki, S Yasuhara, YM Yu, JAJ Martyn, RG Tompkins, T Yorozu, S Inoue, M Kaneki
    FEBS Open Bio, 2019-01-19;9(2):348-363.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  43. Genipin protects against H2O2-induced oxidative damage in�retinal pigment epithelial cells by promoting Nrf2 signaling
    Authors: Hailan Zhao, Ruiqing Wang, Mingxia Ye, Lan Zhang
    International Journal of Molecular Medicine
  44. Validation of antibody-based tools for galanin research
    Authors: SM Brunner, A Koller, J Stockinger, F Locker, S Leis, F Ernst, P Strasser, B Brodowicz, S Ebner, BS Holub, I Rauch, K Graf, R Lang, B Kofler
    Peptides, 2018-09-06;120(0):170009.
    Species: Human, Mouse
    Sample Types: Cell Lysates, Tissue Homogenates
    Applications: Western Blot
  45. Knockdown of anti-silencing function 1B histone chaperone induces cell apoptosis via repressing PI3K/Akt pathway in prostate cancer
    Authors: Guangye Han, Xinjun Zhang, Pei Liu, Quanfeng Yu, Zeyu Li, Qinnan Yu et al.
    International Journal of Oncology
  46. Gelatinolytic activity of autocrine matrix metalloproteinase-9 leads to endothelial de-arrangement in Moyamoya disease
    Authors: KG Blecharz-L, V Prinz, M Burek, D Frey, T Schenkel, SM Krug, M Fromm, P Vajkoczy
    J. Cereb. Blood Flow Metab., 2018-04-10;0(0):271678X187684.
    Species: Human
    Sample Types: Tissue Lysates
    Applications: Western Blot
  47. Caspase-1 Is an Apical Caspase Leading to Caspase-3 Cleavage in the AIM2 Inflammasome Response, Independent of Caspase-8
    Authors: Vitaliya Sagulenko, Nazarii Vitak, Parimala R. Vajjhala, James E. Vince, Katryn J. Stacey
    Journal of Molecular Biology
  48. MicroRNA miR-27 Inhibits Adenovirus Infection by Suppressing the Expression of SNAP25 and TXN2.
    Authors: Machitani M, Sakurai F, Wakabayashi K, Nakatani K, Tachibana M, Mizuguchi H
    J Virol, 2017-05-26;91(12):.
  49. Bim gene dosage is critical in modulating nephron progenitor survival in the absence of microRNAs during kidney development.
    Authors: Cerqueira D, Bodnar A, Phua Y, Freer R, Hemker S, Walensky L, Hukriede N, Ho J
    FASEB J, 2017-04-26;31(8):3540-3554.
  50. Induction of Apoptosis in Human Oral Keratinocyte by Doxorubicin.
    Authors: Sakagami H, Okudaira N, Masuda Y, Amano O, Yokose S, Kanda Y, Suguro M, Natori T, Oizumi H, Oizumi T
    Anticancer Res, 2017-03-01;37(3):1023-1029.
  51. Quantitative Structure-Cytotoxicity Relationship of Chalcones.
    Authors: Sakagami H, Masuda Y, Tomomura M, Yokose S, Uesawa Y, Ikezoe N, Asahara D, Takao K, Kanamoto T, Terakubo S, Kagaya H, Nakashima H, Sugita Y
    Anticancer Res, 2017-03-01;37(3):1091-1098.
  52. Resolution of TLR2-induced inflammation through manipulation of metabolic pathways in Rheumatoid Arthritis
    Authors: T McGarry, M Biniecka, W Gao, D Cluxton, M Canavan, S Wade, S Wade, L Gallagher, C Orr, DJ Veale, U Fearon
    Sci Rep, 2017-02-22;7(0):43165.
  53. Sirtuin1-regulated lysine acetylation of p66Shc governs diabetes-induced vascular oxidative stress and endothelial dysfunction.
    Authors: Kumar S, Kim Y, Vikram A, Naqvi A, Li Q, Kassan M, Kumar V, Bachschmid M, Jacobs J, Kumar A, Irani K
    Proc Natl Acad Sci U S A, 2017-01-30;114(7):1714-1719.
  54. Plasma Membrane Origin of the Steroidogenic Pool of Cholesterol Used in Hormone-induced Acute Steroid Formation in Leydig Cells.
    Authors: Venugopal S, Martinez-Arguelles D, Chebbi S, Hullin-Matsuda F, Kobayashi T, Papadopoulos V
    J Biol Chem, 2016-11-03;291(50):26109-26125.
  55. Hepatic aberrant glycosylation by N-acetylglucosaminyltransferase V accelerates HDL assembly.
    Authors: Kamada Y, Kida S, Hirano K, Yamaguchi S, Suzuki A, Hashimoto C, Kimura A, Sato M, Fujii H, Sobajima T, Yamamoto A, Ebisutani Y, Takamatsu S, Shinzaki S, Yoshida Y, Yamada M, Nagasaka H, Takehara T, Miyoshi E
    Am J Physiol Gastrointest Liver Physiol, 2016-09-22;311(5):G859-G868.
  56. Enhancement of Cytotoxicity of Three Apoptosis-inducing Agents Against Human Oral Squamous Cell Carcinoma Cell Line by Benzoxazinotropone.
    Authors: Tomikoshi Y, Nomura M, Okudaira N, Sakagami H, Wakabayashi H
    In Vivo, 2016-09-10;30(5):645-50.
  57. Cross-regulation of Phosphodiesterase 1 and Phosphodiesterase 2 Activities Controls Dopamine-mediated Striatal alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptor Trafficking.
    Authors: Song R, Tolentino R, Sobie E, Neves-Zaph S
    J Biol Chem, 2016-09-07;291(44):23257-23267.
  58. The ‘melanoma-enriched’ microRNA miR-4731-5p acts as a tumour suppressor
    Authors: Mitchell S. Stark, Lisa N. Tom, Glen M. Boyle, Vanessa F. Bonazzi, H. Peter Soyer, Adrian C. Herington et al.
    Oncotarget
  59. Sustained Mutant KIT Activation in the Golgi Complex Is Mediated by PKC-theta in Gastrointestinal Stromal Tumors.
    Authors: Kim W, Yun S, Park C, Bauer S, Kim J, Lee M, Kim H
    Clin Cancer Res, 2016-07-20;23(3):845-856.
  60. Letrozole vs estradiol valerate induced PCOS in rats: glycemic, oxidative and inflammatory status assessment.
    Authors: Daneasa A, Cucolas C, Lenghel L, Olteanu D, Orasan R, Filip G
    Reproduction, 2016-01-20;151(4):401-9.
  61. MicroRNA Biogenesis and Hedgehog-Patched Signaling Cooperate to Regulate an Important Developmental Transition in Granule Cell Development.
    Authors: Constantin L, Constantin M, Wainwright B
    Genetics, 2016-01-15;202(3):1105-18.
  62. Histone Deacetylase Inhibitors Promote Mitochondrial Reactive Oxygen Species Production and Bacterial Clearance by Human Macrophages.
    Authors: Ariffin J, das Gupta K, Kapetanovic R, Iyer A, Reid R, Fairlie D, Sweet M
    Antimicrob Agents Chemother, 2015-12-28;60(3):1521-9.
  63. Analysis of the N-terminal region of human MLKL, as well as two distinct MLKL isoforms, reveals new insights into necroptotic cell death.
    Authors: Arnez K, Kindlova M, Bokil N, Murphy J, Sweet M, Guncar G
    Biosci Rep, 2015-12-24;36(1):e00291.
  64. Phosphatidylinositol-3,4-Bisphosphate and Its Binding Protein Lamellipodin Regulate Chemotaxis of Malignant B Lymphocytes.
    Authors: Li H, Wu X, Hou S, Malek M, Kielkowska A, Noh E, Makondo K, Du Q, Wilkins J, Johnston J, Gibson S, Lin F, Marshall A
    J Immunol, 2015-12-22;196(2):586-95.
  65. Endoplasmic reticulum stress increases AT1R mRNA expression via TIA-1-dependent mechanism.
    Authors: Backlund M, Paukku K, Kontula K, Lehtonen J
    Nucleic Acids Res, 2015-12-17;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  66. Renal stromal miRNAs are required for normal nephrogenesis and glomerular mesangial survival.
    Authors: Phua Y, Chu J, Marrone A, Bodnar A, Sims-Lucas S, Ho J
    Physiol Rep, 2015-10-01;3(10):.
  67. SORT1 Mutation Resulting in Sortilin Deficiency and p75(NTR) Upregulation in a Family With Essential Tremor.
    Authors: Sanchez E, Bergareche A, Krebs C, Gorostidi A, Makarov V, Ruiz-Martinez J, Chorny A, Lopez de Munain A, Marti-Masso J, Paisan-Ruiz C
    ASN Neuro, 2015-08-21;7(4):.
  68. Alterations of oxidative phosphorylation in meningiomas and peripheral nerve sheath tumors.
    Authors: Feichtinger R, Weis S, Mayr J, Zimmermann F, Bogner B, Sperl W, Kofler B
    Neuro Oncol, 2015-06-23;18(2):184-94.
  69. Decreased eIF3e Expression Can Mediate Epithelial-to-Mesenchymal Transition through Activation of the TGFbeta Signaling Pathway.
    Authors: Desnoyers G, Frost L, Courteau L, Wall M, Lewis S
    Mol Cancer Res, 2015-06-08;13(10):1421-30.
  70. Calcium ions promote primary renal epithelial cell differentiation into cells with bone-associated phenotypes via transforming growth factor-beta 1-induced epithelial-mesenchymal transition in idiopathic hypercalciuria patients
    Authors: DENG HE, SHAOGANG WANG, ZHAOHUI JIA, LEI CUI, YUCHAO LU, HENGLONG HU et al.
    Molecular Medicine Reports
  71. Loss of Gab1 adaptor protein in hepatocytes aggravates experimental liver fibrosis in mice.
    Authors: Kizu T, Yoshida Y, Furuta K, Ogura S, Egawa M, Chatani N, Hamano M, Takemura T, Ezaki H, Kamada Y, Nishida K, Nakaoka Y, Kiso S, Takehara T
    Am J Physiol Gastrointest Liver Physiol, 2015-01-23;308(7):G613-24.
  72. Rab31 and APPL2 enhance FcgammaR-mediated phagocytosis through PI3K/Akt signaling in macrophages.
    Authors: Yeo J, Wall A, Luo L, Stow J
    Mol Biol Cell, 2015-01-07;26(5):952-65.
  73. Tricellulin regulates junctional tension of epithelial cells at tricellular contacts through Cdc42.
    Authors: Oda Y, Otani T, Ikenouchi J, Furuse M
    J Cell Sci, 2014-08-05;127(0):4201-12.
  74. EDEM2 initiates mammalian glycoprotein ERAD by catalyzing the first mannose trimming step.
    Authors: Ninagawa S, Okada T, Sumitomo Y, Kamiya Y, Kato K, Horimoto S, Ishikawa T, Takeda S, Sakuma T, Yamamoto T, Mori K
    J Cell Biol, 2014-08-04;206(3):347-56.
  75. Retrotransposition of long interspersed element 1 induced by methamphetamine or cocaine.
    Authors: Okudaira N, Ishizaka Y, Nishio H
    J Biol Chem, 2014-07-22;289(37):25476-85.
  76. Conditional knockout of the leptin receptor in the colonic epithelium revealed the local effects of leptin receptor signaling in the progression of colonic tumors in mice.
    Authors: Higurashi T, Endo H, Uchiyama T, Uchiyama S, Yamada E, Ohkubo H, Sakai E, Takahashi H, Maeda S, Wada K, Natsumeda Y, Hippo Y, Nakajima A, Nakagama H
    Carcinogenesis, 2014-06-23;35(9):2134-41.
  77. Pharmacological regulation of SPARC by lovastatin in human trabecular meshwork cells.
    Authors: Villarreal G, Chatterjee A, Oh S, Oh D, Rhee D
    Invest Ophthalmol Vis Sci, 2014-03-19;55(3):1657-65.
  78. Forced expression of DNA methyltransferases during oocyte growth accelerates the establishment of methylation imprints but not functional genomic imprinting.
    Authors: Hara S, Takano T, Fujikawa T, Yamada M, Wakai T, Kono T, Obata Y
    Hum Mol Genet, 2014-03-05;23(14):3853-64.
  79. Cortactin scaffolds Arp2/3 and WAVE2 at the epithelial zonula adherens.
    Authors: Han, Siew Pin, Gambin, Yann, Gomez, Guillerm, Verma, Suzie, Giles, Nichole, Michael, Magdalen, Wu, Selwin K, Guo, Zhong, Johnston, Wayne, Sierecki, Emma, Parton, Robert G, Alexandrov, Kirill, Yap, Alpha S
    J Biol Chem, 2014-01-27;289(11):7764-75.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  80. The productive entry pathway of HIV-1 in macrophages is dependent on endocytosis through lipid rafts containing CD4.
    Authors: van Wilgenburg, Bonnie, Moore, Michael, James, William, Cowley, Sally A
    PLoS ONE, 2014-01-22;9(1):e86071.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  81. Lipopolysaccharide induces degradation of connexin43 in rat astrocytes via the ubiquitin-proteasome proteolytic pathway.
    Authors: Liao C, Jeng C, Wang H, Wang S, Wu J
    PLoS ONE, 2013-11-13;8(11):e79350.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  82. Nicotine induces the up-regulation of the alpha7-nicotinic receptor (alpha7-nAChR) in human squamous cell lung cancer cells via the Sp1/GATA protein pathway.
    Authors: Brown K, Perry H, Lau J, Jones D, Pulliam J, Thornhill B, Crabtree C, Luo H, Chen Y, Dasgupta P
    J Biol Chem, 2013-10-02;288(46):33049-59.
  83. Loss of Usp9x Disrupts Cortical Architecture, Hippocampal Development and TGF beta -Mediated Axonogenesis
    Authors: Shane Stegeman, Lachlan A. Jolly, Susitha Premarathne, Jozef Gecz, Linda J. Richards, Alan Mackay-Sim et al.
    PLoS ONE
  84. Mechanism of enhancement of radiation-induced cytotoxicity by sorafenib in colorectal cancer.
    Authors: Kim Y, Jeung H, Jeong I, Lee K, Rha S, Chung H, Kim G
    J Radiat Res, 2012-08-24;54(1):52-60.
  85. Human TSC-associated renal angiomyolipoma cells are hypersensitive to ER stress.
    Authors: Siroky B, Yin H, Babcock J, Lu L, Hellmann A, Dixon B, Quilliam L, Bissler J
    Am J Physiol Renal Physiol, 2012-07-11;303(6):F831-44.
  86. Cell surface expression of the major amyloid-beta peptide (Abeta)-degrading enzyme, neprilysin, depends on phosphorylation by mitogen-activated protein kinase/extracellular signal-regulated kinase kinase (MEK) and dephosphorylation by protein phosphatase 1a.
    Authors: Kakiya N, Saito T, Nilsson P, Matsuba Y, Tsubuki S, Takei N, Nawa H, Saido T
    J Biol Chem, 2012-07-05;287(35):29362-72.
  87. Transcriptional regulation of translocator protein (Tspo) via a SINE B2-mediated natural antisense transcript in MA-10 Leydig cells.
    Authors: Fan J, Papadopoulos V
    Biol Reprod, 2012-05-10;86(5):147, 1-15.
  88. Hormone-induced 14-3-3gamma adaptor protein regulates steroidogenic acute regulatory protein activity and steroid biosynthesis in MA-10 Leydig cells.
    Authors: Aghazadeh Y, Rone M, Blonder J, Ye X, Veenstra T, Hales D, Culty M, Papadopoulos V
    J Biol Chem, 2012-03-16;287(19):15380-94.
  89. Regulation of AT1R expression through HuR by insulin.
    Authors: Paukku K, Backlund M, de Boer R, Kalkkinen N, Kontula K, Lehtonen J
    Nucleic Acids Res, 2012-02-23;40(12):5250-61.
  90. N-Acetylglucosaminyltransferase V regulates TGF-beta response in hepatic stellate cells and the progression of steatohepatitis.
    Authors: Kamada Y, Mori K, Matsumoto H, Kiso S, Yoshida Y, Shinzaki S, Hiramatsu N, Ishii M, Moriwaki K, Kawada N, Takehara T, Miyoshi E
    Glycobiology, 2012-01-30;22(6):778-87.
  91. The nuclear receptor, Nor-1, markedly increases type II oxidative muscle fibers and resistance to fatigue.
    Authors: Pearen M, Eriksson N, Fitzsimmons R, Goode J, Martel N, Andrikopoulos S, Muscat G
    Mol Endocrinol, 2012-01-26;26(3):372-84.
  92. B-cell adaptor for PI3K (BCAP) negatively regulates Toll-like receptor signaling through activation of PI3K.
    Authors: Ni M, MacFarlane A, Toft M, Lowell C, Campbell K, Hamerman J
    Proc Natl Acad Sci U S A, 2011-12-20;109(1):267-72.
  93. Tuberin and PRAS40 are anti-apoptotic gatekeepers during early human amniotic fluid stem-cell differentiation.
    Authors: Fuchs C, Rosner M, Dolznig H, Mikula M, Kramer N, Hengstschlager M
    Hum Mol Genet, 2011-11-16;21(5):1049-61.
  94. MicroRNA-494 downregulates KIT and inhibits gastrointestinal stromal tumor cell proliferation.
    Authors: Kim W, Park M, Kim Y, Tae Y, Yang H, Lee J, Kim H
    Clin Cancer Res, 2011-10-31;17(24):7584-94.
  95. Farnesyltransferase inhibitor FTI-277 reduces mortality of septic mice along with improved bacterial clearance.
    Authors: Yang W, Yamada M, Tamura Y, Chang K, Mao J, Zou L, Feng Y, Kida K, Scherrer-Crosbie M, Chao W, Ichinose F, Yu Y, Fischman A, Tompkins R, Yao S, Kaneki M
    J Pharmacol Exp Ther, 2011-08-26;339(3):832-41.
  96. Protein kinase C and NF-kappaB-dependent CD4 downregulation in macrophages induced by T cell-derived soluble factors: consequences for HIV-1 infection.
    Authors: Raposo RA, Trudgian DC, Thomas B, van Wilgenburg B, Cowley SA, James W
    J. Immunol., 2011-06-10;187(2):748-59.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  97. Patched1 inhibits epidermal progenitor cell expansion and basal cell carcinoma formation by limiting Igfbp2 activity.
    Authors: Villani RM, Adolphe C, Palmer J, Waters MJ, Wainwright BJ
    Cancer Prev Res (Phila), 2010-09-21;3(10):1222-34.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  98. Cadherin adhesion receptors orient the mitotic spindle during symmetric cell division in mammalian epithelia.
    Authors: den Elzen N, Buttery CV, Maddugoda MP, Ren G, Yap AS
    Mol. Biol. Cell, 2009-06-24;20(16):3740-50.
    Species: Canine
    Sample Types: Cell Lysates
    Applications: Western Blot
  99. Post-transcriptional silencing of CCR3 downregulates IL-4 stimulated release of eotaxin-3 (CCL26) and other CCR3 ligands in alveolar type II cells.
    Authors: Taka E, Errahali YJ, Abonyo BO, Bauer DM, Heiman AS
    Cytokine, 2008-11-26;44(3):342-51.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  100. Reduction of brain metastases in plasminogen activator inhibitor-1-deficient mice with transgenic ocular tumors.
    Authors: Maillard CM, Bouquet C, Petitjean MM, Mestdagt M, Frau E, Jost M, Masset AM, Opolon PH, Beermann F, Abitbol MM, Foidart JM, Perricaudet MJ, Noel AC
    Carcinogenesis, 2008-08-27;29(11):2236-42.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  101. Modulation of eotaxin-3 (CCL26) in alveolar type II epithelial cells.
    Authors: Abonyo BO, Lebby KD, Tonry JH, Ahmad M, Heiman AS
    Cytokine, 2007-03-09;36(5):237-44.
  102. Nitric oxide-induced apoptosis in RAW 264.7 macrophages is mediated by endoplasmic reticulum stress pathway involving ATF6 and CHOP.
    Authors: Gotoh T, Oyadomari S, Mori K, Mori M
    J. Biol. Chem., 2002-01-22;277(14):12343-50.
  103. NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis
    Authors: Park MK, Zhang L, Min KW et al.
    Cell metabolism
  104. The Globally Disseminated M1T1 Clone of Group A Streptococcus Evades Autophagy for Intracellular Replication.
    Authors: Barnett TC, Liebl D, Seymour LM et al.
    Cell Host Microbe.
  105. The microRNA‐204‐5p inhibits APJ signalling and confers resistance to cardiac hypertrophy and dysfunction
    Authors: Ravinder Reddy Gaddam, Young‐Rae Kim, Julia S. Jacobs, Jin‐Young Yoon, Qiuxia Li, Angela Cai et al.
    Clinical and Translational Medicine
  106. Impaired differentiation of macrophage lineage cells attenuates bone remodeling and inflammatory angiogenesis in Ndrg1 deficient mice.
    Authors: Watari K, Shibata T, Nabeshima H et al.
    Sci Rep.
  107. Interleukin-1 Induces the Release of Lubricating Phospholipids from Human Osteoarthritic Fibroblast-like Synoviocytes
    Authors: Thottakkattumana Parameswaran, V;Hild, C;Eichner, G;Ishaque, B;Rickert, M;Steinmeyer, J;
    International journal of molecular sciences

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