Human/Mouse/Rat FABP3/H-FABP Antibody Summary
Met1-Ala133
Accession # P05413
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Detection of Human, Mouse, and Rat FABP3/H-FABP antibody by Western Blot. Detection of Human, Mouse, and Rat FABP3/H-FABP antibody by Western Blot.](https://resources.rndsystems.com/images/datasheets/antibody/FABP3_MAB1678_Western_Blot_5986.jpg)
Detection of Human, Mouse, and Rat FABP3/H‑FABP by Western Blot. Western blot shows lysates of human heart tissue, human skeletal muscle tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human/Mouse/Rat FABP3/H-FABP Monoclonal Antibody (Catalog # MAB1678) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for FABP3/H-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
![FABP3/H-FABP antibody in Human Heart by Immunohistochemistry (IHC-P). FABP3/H-FABP antibody in Human Heart by Immunohistochemistry (IHC-P).](https://resources.rndsystems.com/images/datasheets/antibody/FABP3_MAB1678_Immunohistochemistry_11974.jpg)
FABP3/H‑FABP in Human Heart. FABP3/H-FABP was detected in immersion fixed paraffin-embedded sections of human heart using Rat Anti-Human/Mouse/Rat FABP3/H-FABP Monoclonal Antibody (Catalog # MAB1678) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
![Detection of Human and Mouse FABP3/H-FABP antibody by Simple Western<sup>TM</sup>. Detection of Human and Mouse FABP3/H-FABP antibody by Simple Western<sup>TM</sup>.](https://resources.rndsystems.com/images/datasheets/antibody/FABP3_MAB1678_Simple_Western_20571.jpg)
Detection of Human and Mouse FABP3/H‑FABP by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP3/H-FABP at approximately 19 kDa (as indicated) using 5 µg/mL of Rat Anti-Human FABP3/H-FABP Monoclonal Antibody (Catalog # MAB1678) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FABP3/H-FABP
Human FABP3, also known as FABP-Heart (H-FABP), FABP-Muscle (M-FABP) and mammary-derived growth inhibitor (MDGI), is a member of the intracellular FABP family. It plays a role in the transport of long chain fatty acids in the cytoplasm.
Product Datasheets
Citations for Human/Mouse/Rat FABP3/H-FABP Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Tumor-Targeting Peptides: The Functional Screen of Glioblastoma Homing Peptides to the Target Protein FABP3 (MDGI)
Authors: A Ayo, E Figueras, T Schachtsie, M Budak, N Sewald, P Laakkonen
Cancers (Basel), 2020-07-08;12(7):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Fatty acid binding protein 4 in human skeletal muscle.
Authors: Fischer H, Gustafsson T, Sundberg CJ, Norrbom J, Ekman M, Johansson O, Jansson E
Biochem. Biophys. Res. Commun., 2006-05-24;346(1):125-30.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
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