Human/Mouse/Rat Ezrin Antibody

Detection of Human, Mouse, and Rat Ezrin by Western Blot. Western blot shows lysates of human placenta tissue, mouse placenta tissue, and C6 rat glioma cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse/Rat Ezrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7239) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Ezrin at approximately 81 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Ezrin by Simple Western^TM. Simple Western shows lysates of Exosome Standard (HEK293) (NBP3-11684), Exosome Standard (PC‑3) (NBP2-49856), and human cerebellum tissue, loaded at 0.5 mg/ml. A specific band was detected for Ezrin at approximately 76 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human/Mouse/Rat Ezrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7239). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Ezrin in Human Colon Stem Cells. Ezrin was detected in immersion fixed paraffin-embedded sections of human colon stem cells differentiating into ascending colon using Sheep Anti-Human/Mouse/Rat Ezrin Antigen Affinity-purified Polyclonal Antibody (AF7239) at 0.5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010). Chromogranin A was also detected using Mouse Anti-Human Chromogranin A Monoclonal Antibody (MAB90981) at 0.5 µg/mL overnight at 4 °C. Tissue was co-stained using the NorthernLights™ 637-conjugated Anti-Mouse IgG Secondary Antibody (red; NL008) and counterstained with DAPI (blue). Specific staining of Ezrin was localized to microtubules. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Detection of Ezrin in Human Stomach Ezrin was detected in immersion fixed paraffin-embedded sections of human stomach using Sheep Anti-Human/Mouse/Rat Ezrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7239) at 1 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface of epithelial cells in gastric glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.