Human/Mouse/Rat ERK1/ERK2 Antibody

Catalog #: MAB15761
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB15761
MAB15761-SP

Detection of Human, Mouse, and Rat ERK1/ERK2 by Western Blot.

1 Image

All ERK1/ERK2 Antibodies

Product Details

Citations (3)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat ERK1/ERK2 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human ERK1 and ERK2 in direct ELISAs and Western blots. In Western blots, 100% cross-reactivity with recombinant human (rh) ERK2 and no cross-reactivity with rhMAPK4, 6, 7, 9, 10, 11, or 12 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 631122

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human ERK1 synthetic peptide
KKISPFEHQTYCQRTLR
Accession # P27361 (ERK1) & P28482 (ERK2)

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human, Mouse, and Rat ERK1/ERK2 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, BaF3 mouse pro-B cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat ERK1/ERK2 Monoclonal Antibody (Catalog # MAB15761) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for ERK1/ERK2 at approximately 42 and 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ERK1/ERK2

ERK1 and ERK2 (also known as MAPK3 and MAPK1) are 44 and 42 kDa Ser/Thr kinases, respectively. They are part of the Ras-Raf-ERK signal transduction cascade often found downstream of growth factor receptor activation. ERK1 and ERK2 were initially isolated and cloned as kinases activated in response to insulin and NGF. They are expressed in most, if not all, mammalian tissues. Dual threonine and tyrosine phosphorylation activate both ERKs, at Thr202/Tyr204 for human ERK1 and Thr185/Tyr187 for human ERK2.

ERK5, also known as Big Mitogen-activated Protein Kinase 1 (BMK1) and MAPK7, is activated by several mechanisms, including receptor tyrosine kinases, G protein-coupled receptors, and osmotic stress. Like ERK1 and ERK2, ERK5 contains the conserved Thr-Glu-Tyr activation motif in its activation loop. Unlike these ERKs, however, ERK5 contains a unique C-terminal domain that regulates its activation and nuclear translocation.

Long Name

Extracellular Signal-regulated Kinase 1/2

Alternate Names

ERK1; ERK-1; ERK1/ERK2; ERT2; Extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; HS44KDAP; HUMKER1A; Insulin-stimulated MAP2 kinase; MAPK 1; Microtubule-associated protein 2 kinase; mitogen-activated protein kinase 3; p44erk1; p44-ERK1; p44mapk; p44-MAPK; PRKM3

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse/Rat ERK1/ERK2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

Rnd3 Is a Crucial Mediator of the Invasive Phenotype of Glioblastoma Cells Downstream of Receptor Tyrosine Kinase Signalling
Authors: B Almarán, G Ramis, S Fernández, P Villalonga
Cells, 2022-11-22;11(23):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
An engineered IL-2 partial agonist promotes CD8+ T cell stemness
Authors: F Mo, Z Yu, P Li, J Oh, R Spolski, L Zhao, CR Glassman, TN Yamamoto, Y Chen, FM Golebiowsk, D Hermans, S Majri-Morr, LK Picton, W Liao, M Ren, X Zhuang, S Mitra, JX Lin, L Gattinoni, JD Powell, NP Restifo, KC Garcia, WJ Leonard
Nature, 2021-09-15;597(7877):544-548.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Pharmaceutical immunoglobulin G impairs anti-carcinoma activity of oxaliplatin in colon cancer cells
Authors: Y Shang, X Zhang, L Lu, K Jiang, M Krohn, S Matschos, CS Mullins, B Vollmar, D Zechner, P Gong, M Linnebache
British Journal of Cancer, 2021-02-09;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot