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Human/Mouse/Rat Calretinin Antibody

Catalog #: AF5065
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF5065
AF5065-SP
Calretinin in Human Brain.
2 Images
Product Details
Citations (7)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Calretinin Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat Calretinin in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Calretinin
Ala2-Ser177
Accession # AAH15484
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Calretinin antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

Calretinin in Human Brain. Calretinin was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 15 µg/mL Goat Anti-Human/Mouse/ Rat Calretinin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5065) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot Detection of Human/Mouse/Rat Calretinin antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat Calretinin by Western Blot. Western blot shows lysates of human brain, mouse brain and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Calretinin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5065) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for Calretinin at approximately 31 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Calretinin

Calretinin (also 29 kDa Calbindin and Calbindin 2) is a 30 kDa member of the EF-hand family of Ca2+-binding proteins. It serves as both an intracellular calcium sensor and calcium buffer. Calretinin is found in cerebellar granule cells plus cortical bipolar and multipolar GABAergic interneurons.

Human Calretinin is 271 amino acids (aa) in length. It contains six EF-hand domains (aa 16 - 270), each of which show a 12 aa Ca2+-binding motif. There are multiple splice variants. One shows a seven aa substitution for aa 181 - 190, accompanied by a 25 aa substitution for the C-terminal Met. Two others show a one and 14 aa substitution for aa 179 - 271, generating a 20 kDa and 22 kDa isoform, respectively. A fourth variant contains a seven aa substitution for aa 185 - 271. Over aa 2 - 177, human and mouse Calretinin show complete aa identity.

Entrez Gene IDs
794 (Human); 12308 (Mouse); 117059 (Rat)
Alternate Names
29 kDa calbindin; CAB29; CAL2; CALB2; calbindin 2; calbindin 2, (29kD, calretinin); calbindin 2, 29kDa (calretinin); calbindin D29K; Calretinin; CR

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Citations for Human/Mouse/Rat Calretinin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Hepatocyte membrane potential regulates serum insulin and insulin sensitivity by altering hepatic GABA release
    Authors: CE Geisler, S Ghimire, C Hepler, KE Miller, SM Bruggink, KP Kentch, MR Higgins, CT Banek, J Yoshino, S Klein, BJ Renquist
    Cell Reports, 2021-06-29;35(13):109298.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Is the eye a window to the brain in Sanfilippo syndrome?
    Authors: H Beard, G Chidlow, D Neumann, N Nazri, M Douglass, PJ Trim, MF Snel, RJ Casson, KM Hemsley
    Acta Neuropathol Commun, 2020-11-17;8(1):194.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. PlexinA1 is crucial for the midline crossing of callosal axons during corpus callosum development in BALB/cAJ mice
    Authors: MM Hossain, T Tsuzuki, K Sakakibara, F Imaizumi, A Ikegaya, M Inagaki, I Takahashi, T Ito, H Takamatsu, A Kumanogoh, T Negishi, K Yukawa
    PLoS ONE, 2019-08-20;14(8):e0221440.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Estrogen treatment reverses prematurity-induced disruption in cortical interneuron population
    Authors: S Panda, P Dohare, S Jain, N Parikh, P Singla, R Mehdizadeh, DW Klebe, GM Kleinman, B Cheng, P Ballabh
    J. Neurosci., 2018-07-23;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. AAV2-Mediated Transduction of the Mouse Retina After Optic Nerve Injury
    Authors: RW Nickells, HM Schmitt, ME Maes, CL Schlamp
    Invest. Ophthalmol. Vis. Sci., 2017-12-01;58(14):6091-6104.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. The nucleus reuniens: a key node in the neurocircuitry of stress and depression
    Authors: V Kafetzopou, N Kokras, I Sotiropoul, JF Oliveira, H Leite-Alme, A Vasalou, VM Sardinha, Z Papadopoul, OF Almeida, K Antoniou, N Sousa, C Dalla
    Mol. Psychiatry, 2017-04-11;0(0):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  7. Dmrt factors determine the positional information of cerebral cortical progenitors via differential suppression of homeobox genes
    Authors: Daijiro Konno, Chiaki Kishida, Kazumitsu Maehara, Yasuyuki Ohkawa, Hiroshi Kiyonari, Seiji Okada et al.
    Development

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