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Human/Mouse/Rat beta-Catenin Antibody

Catalog #: AF1329
31 Citations 4 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1329
AF1329-SP
Detection of Human/Mouse/Rat beta ‑Catenin by Western Blot.
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Product Details
Citations (31)
FAQs
Supplemental Products
Reviews (4)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat beta-Catenin Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse and rat beta -Catenin in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human beta -Catenin
Ala2-Leu781
Accession # P35222
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
50 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Chromatin Immunoprecipitation (ChIP)
5 µg/5 x 106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat beta ‑Catenin antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat beta ‑Catenin by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C6 rat glioma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse/Rat beta -Catenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1329) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human beta -catenin (1 ng) was included. A specific band for beta-Catenin was detected at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Chromatin Immunoprecipitation (ChIP) Detection of beta -Catenin-regul-ated Genes antibody by Chromatin Immunoprecipitation. View Larger

Detection of beta ‑Catenin-regul-ated Genes by Chromatin Immunoprecipitation. HeLa human cervical epithelial carcinoma cell line were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. beta -Catenin/ DNA complexes were immuno-precipitated using 5 µg Goat Anti-Human/Mouse/Rat beta -Catenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1329) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immuno-complexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. TheSU(Z)12promoter was detected by standard PCR.

Immunocytochemistry beta -Catenin antibody in SW480 Human Cell Line by Immunocytochemistry (ICC). View Larger

beta ‑Catenin in SW480 Human Cell Line. beta -Catenin was detected in immersion fixed SW480 human colorectal adenocarcinoma cell line using Goat Anti-Human/Mouse/Rat beta -Catenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1329) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry beta -Catenin antibody in Human Kidney Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

beta -Catenin in Human Kidney Cancer Tissue. beta -Catenin was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human/Mouse/Rat beta -Catenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1329) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry beta -Catenin antibody in Human Kidney Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

beta ‑Catenin in Human Kidney Cancer Tissue. beta -Catenin was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using 15 µg/mL Goat Anti-Human/Mouse/Rat beta -Catenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1329) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to epithelial cells in collecting tubules in the medulla. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Intracellular Staining by Flow Cytometry Detection of beta -Catenin antibody in HeLa Human Cell Line antibody by Flow Cytometry. View Larger

Detection of beta ‑Catenin in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Goat Anti-Human/Mouse/Rat beta -Catenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1329, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Simple Western Detection of Human and Mouse beta ‑Catenin antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human and Mouse beta ‑Catenin by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for beta -Catenin at approximately 94-97 kDa (as indicated) using 50 µg/mL of Goat Anti-Human/Mouse/Rat beta -Catenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1329) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-Catenin

beta -Catenin is a cadherin-associated protein that is involved in the regulation of cell adhesion. It also acts as a transcriptional co-activator in the nucleus and is involved the canonical Wnt signal transduction pathways.

Entrez Gene IDs
1499 (Human); 12387 (Mouse); 84353 (Rat)
Alternate Names
bCatenin; beta 1 (88kD); beta-Catenin; catenin (cadherin-associated protein), beta 1, 88kDa; catenin beta-1; CTNNB; CTNNB1; DKFZp686D02253; FLJ25606; FLJ37923

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Citations for Human/Mouse/Rat beta-Catenin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

31 Citations: Showing 1 - 10
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  1. Annexin A2 supports pulmonary microvascular integrity by linking vascular endothelial cadherin and protein tyrosine phosphatases
    Authors: Luo M, Flood EC, Almeida D et al.
    J. Exp. Med.
  2. A Fat4-Dchs1 signal between stromal and cap mesenchyme cells influences nephrogenesis and ureteric bud branching.
    Authors: Mao Y, Francis-West P, Irvine KD.
    Development.
  3. Wnt/ beta -catenin links oxidative stress to podocyte injury and proteinuria
    Authors: Lili Zhou, Xiaowen Chen, Meizhi Lu, Qinyu Wu, Qian Yuan, Chengxiao Hu et al.
    Kidney International
  4. GSK3beta Inhibition Reduced Vascular Calcification in Ins2Akita/+ Mice
    Authors: KI Boström, X Qiao, Y Zhao, X Wu, L Zhang, JA Ma, J Ji, X Cai, Y Yao
    International Journal of Molecular Sciences, 2023-03-22;24(6):.
  5. Aberrant nonfibrotic parenchyma in idiopathic pulmonary fibrosis is correlated with decreased beta -catenin inhibition and increased Wnt5a/b interaction
    Authors: Kristina Rydell-Törmänen, Xiao-Hong Zhou, Oskar Hallgren, Jonas Einarsson, Leif Eriksson, Annika Andersson-Sjöland et al.
    Physiological Reports
  6. GSK3 beta Inhibition Ameliorates Atherosclerotic Calcification
    Authors: Cai, X;Zhao, Y;Yang, Y;Wu, X;Zhang, L;Ma, JA;Ji, J;Boström, KI;Yao, Y;
    International journal of molecular sciences
    Species: Mouse
    Sample Types: Cell Lysates, Tissue Homogenates
    Applications: Western Blot
  7. TCF/LEF regulation of the topologically associated domain ADI promotes mESCs to exit the pluripotent ground state
    Authors: N Doumpas, S Söderholm, S Narula, S Moreira, BW Doble, C Cantù, K Basler
    Cell Reports, 2021-09-14;36(11):109705.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Shifting osteogenesis in vascular calcification
    Authors: J Yao, X Wu, X Qiao, D Zhang, L Zhang, JA Ma, X Cai, KI Boström, Y Yao
    JCI Insight, 2021-05-24;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates, Tissue Homogenates
    Applications: ChIP, ChIP-Seq, Western Blot
  9. LncRNA SNHG6 regulating Hedgehog signaling pathway and affecting the biological function of gallbladder carcinoma cells through targeting miR-26b-5p
    Authors: XF Liu, K Wang, HC Du
    Eur Rev Med Pharmacol Sci, 2020-07-01;24(14):7598-7611.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. The Actin-Family Protein Arp4 Is a Novel Suppressor for the Formation and Functions of Nuclear F-Actin
    Authors: S Yamazaki, C Gerhold, K Yamamoto, Y Ueno, R Grosse, K Miyamoto, M Harata
    Cells, 2020-03-19;9(3):.
    Species: Mouse
    Sample Types: Cell Culture Lysates
    Applications: Immunoprecipitation
  11. Progranulin protects the mouse retina under hypoxic conditions via inhibition of the Toll?like receptor?4?NADPH oxidase 4 signaling pathway
    Authors: ZP You, MJ Yu, YL Zhang, K Shi
    Mol Med Rep, 2018-11-08;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  12. Amnionless-mediated glycosylation is crucial for cell surface targeting of cubilin in renal and intestinal cells
    Authors: T Udagawa, Y Harita, K Miura, J Mitsui, KL Ode, S Morishita, S Urae, S Kanda, Y Kajiho, H Tsurumi, HR Ueda, S Tsuji, A Saito, A Oka
    Sci Rep, 2018-02-05;8(1):2351.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  13. WNT10A mutation causes ectodermal dysplasia by impairing progenitor cell proliferation and KLF4-mediated differentiation
    Authors: M Xu, J Horrell, M Snitow, J Cui, H Gochnauer, CM Syrett, S Kallish, JT Seykora, F Liu, D Gaillard, JP Katz, KH Kaestner, B Levin, C Mansfield, JE Douglas, BJ Cowart, M Tordoff, F Liu, X Zhu, LA Barlow, AI Rubin, JA McGrath, EE Morrisey, EY Chu, SE Millar
    Nat Commun, 2017-06-07;8(0):15397.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  14. Dchs1-Fat4 regulation of polarized cell behaviours during skeletal morphogenesis
    Nat Commun, 2016-05-05;7(0):11469.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. ROS-induced endothelial stress contributes to pulmonary fibrosis through pericytes and Wnt signaling.
    Authors: Andersson-Sjoland A, Karlsson J, Rydell-Tormanen K
    Lab Invest, 2015-09-14;96(2):206-17.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  16. WNT1-induced Secreted Protein-1 (WISP1), a Novel Regulator of Bone Turnover and Wnt Signaling.
    Authors: Maeda A, Ono M, Holmbeck K, Li L, Kilts T, Kram V, Noonan M, Yoshioka Y, McNerny E, Tantillo M, Kohn D, Lyons K, Robey P, Young M
    J Biol Chem, 2015-04-11;290(22):14004-18.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-P, Western Blot
  17. Integrin beta1 controls VE-cadherin localization and blood vessel stability.
    Authors: Yamamoto H, Ehling M, Kato K, Kanai K, van Lessen M, Frye M, Zeuschner D, Nakayama M, Vestweber D, Adams R
    Nat Commun, 2015-03-10;6(0):6429.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Immunoprecipitation, Western Blot
  18. Osteocytes mediate the anabolic actions of canonical Wnt/beta-catenin signaling in bone.
    Authors: Tu X, Delgado-Calle J, Condon K, Maycas M, Zhang H, Carlesso N, Taketo M, Burr D, Plotkin L, Bellido T
    Proc Natl Acad Sci U S A, 2015-01-20;112(5):E478-86.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  19. The Wnt/beta-catenin pathway attenuates experimental allergic airway disease.
    Authors: Reuter S, Martin H, Beckert H, Bros M, Montermann E, Belz C, Heinz A, Ohngemach S, Sahin U, Stassen M, Buhl R, Eshkind L, Taube C
    J Immunol, 2014-06-13;193(2):485-95.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  20. High glucose induces podocyte injury via enhanced (pro)renin receptor-Wnt-beta-catenin-snail signaling pathway.
    Authors: Li C, Siragy H
    PLoS ONE, 2014-02-12;9(2):e89233.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. Shaping organs by a wingless-int/Notch/nonmuscle myosin module which orients feather bud elongation.
    Authors: Li A, Chen M, Jiang T, Wu P, Nie Q, Widelitz R, Chuong C
    Proc Natl Acad Sci U S A, 2013-04-01;110(16):E1452-61.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: Bioassay
  22. Canonical Wnt signaling negatively regulates platelet function.
    Authors: Steele BM, Harper MT, Macaulay IC, Morrell CN, Perez-Tamayo A, Foy M, Habas R, Poole AW, Fitzgerald DJ, Maguire PB
    Proc. Natl. Acad. Sci. U.S.A., 2009-11-09;106(47):19836-41.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  23. Blockade of Wnt signaling inhibits angiogenesis and tumor growth in hepatocellular carcinoma.
    Authors: Hu J, Dong A, Fernandez-Ruiz V, Shan J, Kawa M, Martinez-Anso E, Prieto J, Qian C
    Cancer Res., 2009-08-18;69(17):6951-9.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  24. Mutational analysis of the FXNPXY motif within LDL receptor-related protein 1 (LRP1) reveals the functional importance of the tyrosine residues in cell growth regulation and signal transduction.
    Authors: Zhang H, Lee JM, Wang Y, Dong L, Ko KW, Pelletier L, Yao Z
    Biochem. J., 2008-01-01;409(1):53-64.
    Species: Hamster
    Sample Types: Cell Lysates
    Applications: Western Blot
  25. Dickkopf-1 is a master regulator of joint remodeling.
    Authors: Diarra D, Stolina M, Polzer K, Zwerina J, Ominsky MS, Dwyer D, Korb A, Smolen J, Hoffmann M, Scheinecker C, van der Heide D, Landewe R, Lacey D, Richards WG, Schett G
    Nat. Med., 2007-01-21;13(2):156-63.
    Species: Human, Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  26. Requirements for proximal tubule epithelial cell detachment in response to ischemia: role of oxidative stress.
    Authors: Saenz-Morales D, Escribese MM, Stamatakis K, Garcia-Martos M, Alegre L, Conde E, Perez-Sala D, Mampaso F, Garcia-Bermejo ML
    Exp. Cell Res., 2006-09-12;312(19):3711-27.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  27. Interlocked positive and negative feedback network motifs regulate beta -catenin activity in the adherens junction pathway
    Authors: David J. Klinke, Nicholas Horvath, Vanessa Cuppett, Yueting Wu, Wentao Deng, Rania Kanj
    Molecular Biology of the Cell
  28. Dopamine D2 receptor modulates Wnt expression and control of cell proliferation
    Authors: Fei Han, Prasad Konkalmatt, Chaitanya Mokashi, Megha Kumar, Yanrong Zhang, Allen Ko et al.
    Scientific Reports
  29. A Selective Neutraligand for CXCL12/SDF-1 alpha With Beneficial Regulatory Functions in MRL/Lpr Lupus Prone Mice
    Authors: Nicolas Schall, François Daubeuf, Claire Marsol, Patrick Gizzi, Nelly Frossard, Dominique Bonnet et al.
    Frontiers in Pharmacology
  30. Zeylenone represses the progress of human prostate cancer by downregulating the Wnt/??catenin pathway
    Authors: S Zeng, B Zhu, J Zeng, W Wu, C Jiang
    Mol Med Rep, 2018-10-17;0(0):.
  31. A Dual Array-Based Approach to Assess the Abundance and Posttranslational Modification State of Signaling Proteins
    Authors: Katrin Luckert, Taranjit S. Gujral, Marina Chan, Mark Sevecka, Thomas O. Joos, Peter K. Sorger et al.
    Science Signaling

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Reviews for Human/Mouse/Rat beta-Catenin Antibody

Average Rating: 4.5 (Based on 4 Reviews)

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Human/Mouse/Rat beta-Catenin Antibody
By Anonymous on 06/27/2021
Application: IHC Sample Tested: epithelium Species: Mouse
Immunohistochemistry Human/Mouse/Rat beta-Catenin Antibody AF1329
Human/Mouse/Rat beta-Catenin Antibody
By Tina Yuan on 11/23/2020
Application: IHC Sample Tested: mouse oral epithelium Species: Mouse
Immunohistochemistry Human/Mouse/Rat beta-Catenin Antibody AF1329
Human/Mouse/Rat beta -Catenin Antibody
By Anonymous on 11/30/2018
Application: Immunocytochemistry/Immunofluorescence Sample Tested: c2bbe Species: Human
Human/Mouse/Rat beta -Catenin Antibody
By Anonymous on 01/25/2018
Application: WB Sample Tested: IPS2 induced pluripotent stem cells Species: Mouse
Western Blot Human/Mouse/Rat beta -Catenin Antibody AF1329