Home / Annexin V / Human/Mouse/Rat Annexin V Antibody

Human/Mouse/Rat Annexin V Antibody

Catalog #: AF399
9 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF399
AF399-SP
Detection of Human, Mouse, and Rat Annexin V by Western Blot.
2 Images
Product Details
Citations (9)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Annexin V Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse and rat Annexin V in Western blots. Detects human Annexin V in direct ELISAs.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Annexin V
Ala2-Asp320
Accession # P08758
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
12.5 µg/mL
Human lung, mouse lung, rat lung

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat Annexin V antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat Annexin V by Western Blot. Western blot shows lysates of human lung tissue, mouse lung tissue, and rat lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Annexin V Antigen Affinity-purified Polyclonal Antibody (Catalog # AF399) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Annexin V at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western View Larger

Detection of Human, Mouse, Rat Annexin V by Simple WesternTM. Simple Western lane view shows lysates of Human lung, mouse lung, rat lung, loaded at 0.2 mg/mL. A specific band was detected for Annexin V at approximately 38 kDa (as indicated) using 12.5 µg/mL of Goat Anti-Human/Mouse/Rat Annexin V Antigen Affinity-purified Polyclonal Antibody (Catalog # AF399). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Annexin V

Annexin V is a member of a calcium and phospholipid binding family of proteins with vascular anticoagulant activity. It is also known as PP4, PAP I, CPB-I, CaBP33, VACa, anchorin CII, lipocortin-V, endonexin II, and thromboplastin inhibitor.

Entrez Gene IDs
308 (Human)
Alternate Names
Anchorin CII; annexin A5; Annexin V; annexin-5; ANX5; ANXA5; ANXV; Calphobindin I; Endonexin II; ENX2CBP-I; Lipocortin V; PAP-I; Placental anticoagulant protein 4; Placental anticoagulant protein I; PP4; Thromboplastin inhibitor; VAC-alpha; Vascular anticoagulant-alpha

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human/Mouse/Rat Annexin V Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
Filter your results:

Filter by:

  1. Individually cultured bovine embryos produce extracellular vesicles that have the potential to be used as non-invasive embryo quality markers
    Authors: K Dissanayak, M Nõmm, F Lättekivi, Y Ressaissi, K Godakumara, A Lavrits, G Midekessa, J Viil, R Bæk, MM Jørgensen, S Bhattachar, A Andronowsk, A Salumets, Ü Jaakma, A Fazeli
    Theriogenology, 2020-04-04;149(0):104-116.
    Species: Mouse
    Sample Types:
    Applications: Microarray
  2. Platelets, endothelial cells and leukocytes contribute to the exercise-triggered release of extracellular vesicles into the circulation
    Authors: Alexandra Brahmer, Elmo Neuberger, Leona Esch-Heisser, Nils Haller, Malene Moeller Jorgensen, Rikke Baek et al.
    Journal of Extracellular Vesicles
  3. Induction of a Regulatory Phenotype in CD3+ CD4+ HLA-DR+ T Cells after Allogeneic Mixed Lymphocyte Culture; Indications of Both Contact-Dependent and -Independent Activation
    Authors: Anne Louise Schacht Revenfeld, Rikke Bæk, Malene Møller Jørgensen, Kim Varming, Allan Stensballe
    International Journal of Molecular Sciences
  4. Megf10 Is a Receptor for C1Q That Mediates Clearance of Apoptotic Cells by Astrocytes
    Authors: Joseph El Khoury
    J. Neurosci., 2016-05-11;36(19):5185-92.
    Species: Mouse
    Sample Types: Whole Cells
  5. Phenotyping of Leukocytes and Leukocyte-Derived Extracellular Vesicles
    Authors: Lotte Hatting Pugholm, Rikke Bæk, Evo Kristina Lindersson Søndergaard, Anne Louise Schacht Revenfeld, Malene Møller Jørgensen, Kim Varming
    Journal of Immunology Research
  6. The tolerogenic function of annexins on apoptotic cells is mediated by the annexin core domain.
    Authors: Linke B, Abeler-Dorner L, Jahndel V, Kurz A, Mahr A, Pfrang S, Linke L, Krammer P, Weyd H
    J Immunol, 2015-04-27;194(11):5233-42.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Type II collagen levels correlate with mineralization by articular cartilage vesicles.
    Authors: Jubeck B, Muth E, Gohr CM, Rosenthal AK
    Arthritis Rheum., 2009-09-01;60(9):2741-6.
    Species: Porcine
    Sample Types: Whole Tissue
    Applications: Western Blot
  8. Proteomic assessment of sympathetic ganglia from adult mice that possess null mutations of ExonIII or ExonIV in the p75 neurotrophin receptor gene.
    Authors: McDonald TG, Scott SA, Kane KM, Kawaja MD
    Brain Res., 2008-11-18;1253(0):1-14.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  9. Potentials and capabilities of the Extracellular Vesicle (EV) Array.
    Authors: Jorgensen MM, Baek R, Varming K.
    J Extracell Vesicles

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human/Mouse/Rat Annexin V Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse/Rat Annexin V Antibody and earn rewards!

Have you used Human/Mouse/Rat Annexin V Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review