Human/Mouse/Rat 14-3-3 eta Antibody

Catalog #: AF4420
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4420
AF4420-SP

Detection of Human/Mouse/ Rat 14‑3‑3 eta by Western Blot.

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All 14-3-3 eta Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat 14-3-3 eta Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat 14-3-3 eta in Western blots. In Western Blot less than 1% cross reactivity with other recombinant human 14-3-3 isoforms.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human 14‑3‑3 eta
Met1-Asn246
Accession # Q04917

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/ Rat 14‑3‑3 eta by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/ Mouse/Rat 14-3-3 eta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4420) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human 14-3-3 beta, epsilon, eta, gamma, sigma, theta, and zeta (2 ng/lane) were included. A specific band for 14-3-3 eta was detected at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 14-3-3 eta

14-3-3 proteins are a highly conserved family of homo- and heterodimeric phosphoserine/threonine-binding proteins present in all eukaryotic cells. 14-3-3 proteins act as key regulators of intracellular signal transduction through their ability to bind specific motifs phosphorylated on serine or threonine. There are at least 7 distinct mammalian 14-3-3 genes: alpha / beta, epsilon, eta, gamma, tau / theta, sigma, and zeta /δ.

Entrez Gene IDs

7533 (Human); 22629 (Mouse); 25576 (Rat)

Alternate Names

1433 eta; 14-3-3 eta; 14-3-3 protein eta; Protein AS1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, etapolypeptide; YWHA1; YWHA114-3-3 eta; YWHAH

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human/Mouse/Rat 14-3-3 eta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

YWHA (14-3-3) protein isoforms and their interactions with CDC25B phosphatase in mouse oogenesis and oocyte maturation
Authors: AA Eisa, S De, A Detwiler, E Gilker, AC Ignatious, S Vijayaragh, D Kline
BMC Dev. Biol., 2019-10-22;19(1):20.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot