Human/Mouse Pentraxin 2/SAP Antibody Summary
Gln21-Asp224
Accession # P12246
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human and Mouse Pentraxin 2/SAP by Western Blot. Western blot shows lysates of mouse kidney tissue, mouse liver tissue, and human kidney tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse Pentraxin 2/SAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2558) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Pentraxin 2/SAP at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Mouse Pentraxin 2/SAP by Simple WesternTM. Simple Western lane view shows lysates of mouse kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for Pentraxin 2/SAP at approximately 36 kDa (as indicated) using 2 µg/mL of Sheep Anti-Human/Mouse Pentraxin 2/SAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2558) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Pentraxin 2/SAP
Pentraxin 2 (also known as Serum Amyoid P Component or SAP) is a secreted glycoprotein that is a universal non-fibrillar component of amyloid deposits. Amyloid is an abnormal extracellular deposit of insoluble protein fibrils that can lead to tissue damage and disease (1‑3). Pentraxin 2 belongs to the pentraxin (pentaxin) family, whose members have a characteristic pentagonal discoid arrangement of five non-covalently bound subunits (4). Pentraxin domains contain the consensus sequence, HxCx(S/T)WxS (x = any amino acid), a lectin fold, and two calcium-binding sites (1). They bind to a variety of unrelated molecules in a calcium-dependent lectin-like manner (1, 4, 5). Pentraxin 2 and C-reactive protein (CRP) are members of the classical or short pentraxin subfamily and share 46% amino acid (aa) identity (1). Mouse Pentraxin 2 is the major acute-phase protein whose expression is dependent on complement activation, IL-6 and/or IL-1 beta, while in humans, CRP is the major acute-phase protein (2, 5, 9). Both are produced and secreted by liver hepatocytes and circulate in plasma. The 204 aa mature mouse Pentraxin 2 shares 79% aa identity with rat Pentraxin 2 and 63‑70% aa identity with human, guinea pig, golden hamster, porcine, and bovine Pentraxin 2 (2, 5). Amyloid deposits containing Pentraxin 2 are implicated in a diverse range of diseases including Alzheimer’s, prion diseases, type 2 diabetes and various systemic amyloidoses (3, 6, 7). Pentraxin 2 regulates the solubility of amyloid fibrils and protects them from degradation. In addition to its pathogenic role, Pentraxin 2 also has an important physiological function in innate immunity (8). It is an opsonin that interacts with all three types of human Fc gamma receptors that mediate neutrophil phagocytosis (8). Pentraxin 2 has been proposed to bind and sequester a variety of ligands including auto-antigens, apoptotic cells, chromatin, DNA, and micro-organisms (1‑3). Pentraxin 2 is also a normal component of basement membranes (1).
- Garlanda, C. et al. (2005) Annu. Rev. Immunol. 23:337.
- Steel, D. and A. Whitehead (1994) Immunol. Today 15:81.
- Alexandrescu, A.T. (2005) Protein Sci. 14:1.
- Emsley, J. et al. (1994) Nature 367:338.
- Mole, J.E. et al. (1988) J. Immunol. 141:3642.
- Gillmore, J.D. et al. (2004) Immunology 112:255.
- Pepys, M. et al. (2002) Nature 417:254.
- Bharadwaj, D. et al. (2001) J. Immunol. 166:6735.
- Szalai, A.J. et al. (2000) J. Immunol. 165:1030.
Product Datasheets
Citations for Human/Mouse Pentraxin 2/SAP Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Plasma redox imbalance caused by albumin oxidation promotes lung-predominant NETosis and pulmonary cancer metastasis
Authors: M Inoue, R Nakashima, M Enomoto, Y Koike, X Zhao, K Yip, SH Huang, JN Waldron, M Ikura, FF Liu, SV Bratman
Nat Commun, 2018-11-30;9(1):5116.
Species: Human
Sample Types: Plasma
Applications: Western Blot -
Persistent lung inflammation and fibrosis in serum amyloid P component (APCs-/-) knockout mice.
Authors: Pilling, Darrell, Gomer, Richard
PLoS ONE, 2014-04-02;9(4):e93730.
Species: Mouse
Sample Types: Serum
Applications: Western Blot -
Reduction of bleomycin-induced pulmonary fibrosis by serum amyloid P.
Authors: Pilling D, Roife D, Wang M, Ronkainen SD, Crawford JR, Travis EL, Gomer RH
J. Immunol., 2007-09-15;179(6):4035-44.
Species: Mouse
Sample Types: Recombinant Protein
Applications: Western Blot
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