Human/Mouse HMGB3 Antibody

Catalog #: MAB5507
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5507
MAB5507-SP

Detection of Human HMGB3 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse HMGB3 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse HMGB3 in Western blots.

Source

Monoclonal Mouse IgG_2A Clone # 546737

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human HMGB3
Met1-Val180
Accession # O15347

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.05 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human HMGB3 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 0.05 µg/mL Mouse Anti-Human/Mouse HMGB3 Monoclonal Antibody (Catalog # MAB5507) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for HMGB3 was detected at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HMGB3

High mobility group protein B3 (HMGB3; also HMG-4 and HMG-2a) is a 23 kDa (predicted) nuclear protein and member of the HMGB family. Human HMGB3 is synthesized as a 200 amino acid precursor, which is demethylated to produce the 199 aa mature chain. The protein contains two HMG box DNA-binding domains (aa 9‑79 and 93‑161) and an acidic Asp/Glu-rich region (aa 181‑200). Human HMGB3 shares 98% aa sequence identity with mouse and bovine HMGB3. HMGB3 is expressed predominantly in the placenta. It binds preferentially single‑stranded DNA and unwinds double stranded DNA.

Long Name

High Mobility Group Box 3

Entrez Gene IDs

3149 (Human); 15354 (Mouse); 305373 (Rat)

Alternate Names

High mobility group protein 2a; High mobility group protein 4; high mobility group protein B3; high-mobility group (nonhistone chromosomal) protein 4; high-mobility group box 3; HMG2A; HMG-2a; HMG2AHMG-4; HMG4; HMG4MGC90319; HMGB3; non-histone chromosomal protein

Product Datasheets

Product Datasheet

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Related Research Areas

Citation for Human/Mouse HMGB3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1