Human/Mouse Heparan Sulfate 3‑O‑Sulfotransferase 4/HS3ST4 Antibody

Catalog #: MAB60851
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB60851
MAB60851-SP

Detection of Human and Mouse Heparan Sulfate 3‑O‑Sulfotransferase 4/HS3ST4 by Western Blot.

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Human/Mouse Heparan Sulfate 3‑O‑Sulfotransferase 4/HS3ST4 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 in direct ELISAs, and human and mouse HS3ST4 in Western blots. In Western blots, no cross-reactivity with recombinant human HS3ST3B1 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 712011

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4
Gly184-Lys456
Accession # Q9Y661

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse Heparan Sulfate 3‑O‑Sulfotransferase 4/HS3ST4 by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, Neuro-2A mouse neuroblastoma cell line, U-118-MG human glioblastoma/astrocytoma cell line, and human brain (hippocampus) tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 Monoclonal Antibody (Catalog # MAB60851) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4

Heparan sulfate is a highly sulfated polysaccharide that can be found on the cell surface and within the extracellular matrix. It is typically covalently attached to the protein core of proteoglycans, such as syndecans and glypicans. Heparin, on the other hand, can be considered as a highly sulfated version of heparan sulfate that is predominantly found in mast cells. Both heparin and heparan sulfate contain disaccharide repeats of uronic acid and N‑acetylglucosamine and are modified by the same sulfotransferases (1, 2). The uronic acid residues can be sulfated at the 2-O position by heparan sulfate 2-O sulfotransferase (HS2ST). The N‑acetylglucosamine residues can be sulfated at the N, 3-O, and 6-O positions by N‑deacetylase/N‑sulfotransferases (NDSTs), heparan sulfate 3-O sulfotransferases (HS3STs) and heparan sulfate 6-O sulfotransferases (HS6STs) respectively. There are seven HS3STs in the human genome (3, 4). HS3ST4 and HS3ST2 are brain specific and may participate in HS-dependent neurobiologic events (5). HS3ST4 can generate tetrasulfated heparan sulfate disaccharide, the most highly sulfated sugar found in biological samples (6, 7), and may have a role in assisting HSV-1 entry and spread (8). HS3ST4 is a Golgi resident type II membrane protein and has the longest proline rich stem region among all HS3STs (3, 5).

References

Bernfield, M. et al. (1999) Annu. Rev. Biochem. 68:729.
Esko, J.D. and Selleck, S.B. (2002) Annu. Rev. Biochem. 71:435.
Shworak, N.W. et al. (1999) J. Biol. Chem. 274:5170.
Xu, D. et al. (2005) Biochem. J. 386:451.
Lawrence, R. et al. (2007) Matrix Biol. 26:442.
Mochizuki, H. et al. (2003) J. Biol. Chem. 278:26780.
Wu, Z.L. et al. (2004) J. Biol. Chem. 279:1861.
Tiwari,V. et al. (2005) Biochem. Biophys. Res. Commun. 338:930.

Entrez Gene IDs

9951 (Human)

Alternate Names

30ST4; 3OST4 heparan sulfate 3-O-sulfotransferase-4; 3-OST-4; EC 2.8.2; EC 2.8.2.23; h3-OST-4; heparan sulfate (glucosamine) 3-O-sulfotransferase 4; Heparan sulfate 3-O-sulfotransferase 4; Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 4; heparan sulfate glucosamine 3-O-sulfotransferase 4; HS3ST4

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Citation for Human/Mouse Heparan Sulfate 3‑O‑Sulfotransferase 4/HS3ST4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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