Human/Mouse Frizzled-1 APC-conjugated Antibody

Catalog #: FAB11201A
1 Citation Datasheet / COA / SDS

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FAB11201A

Detection of Frizzled‑1 in HEK293 Human Cell Line transfected with Human Frizzled-1 by Flow Cytometry.

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All Frizzled-1 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Frizzled-1 APC-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects mouse Frizzled-1 in direct ELISAs and Western blots. Detects human and mouse Frizzled-1 in Flow Cytometry. In direct ELISAs, approximately 30% cross‑reactivity with recombinant mouse (rm) Frizzled-9 is observed and no cross-reactivity with recombinant human (rh) Frizzled-5, rmFrizzled 2, 3, 4, 6, 7, 8, or rhMFRP is observed. No staining is observed on normal human PBMCs.

Source

Monoclonal Rat IgG_2A Clone # 162531

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Frizzled‑1
Gln1-His248 (Met122Ile)
Accession # O70421

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

see below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Frizzled‑1 in HEK293 Human Cell Line transfected with Human Frizzled-1 by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with Frizzled-1 was stained with Rat Anti-Human/Mouse Frizzled-1 APC-conjugated Monoclonal Antibody (Catalog # FAB11201A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of Frizzled‑1 in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line treated with Retinoic Acid for 3 days was stained with Rat Anti-Human/Mouse Frizzled-1 APC-conjugated Monoclonal Antibody (Catalog # FAB11201A, filled histogram) or isotype control antibody (IC006A, open histogram). Staining was performed using our Staining Membrane-associated Proteins protocol.

Flow Cytometry

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Detection of Frizzled‑1 in HEK293 Human Cell Line by Flow Cytometry. HEK293 human embryonic kidney cell line was stained with Rat Anti-Human/Mouse Frizzled-1 APC-conjugated Monoclonal Antibody (Catalog # FAB11201A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of Frizzled‑1 in NS0 cell line transfected with mouse Frizzled-1 by Flow Cytometry NS0 cell line transfected with mouse Frizzled-1 were stained with Rat Anti-Human/Mouse Frizzled‑1 APC‑conjugated Monoclonal Antibody (Catalog # FAB11201A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with saponin. View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Frizzled-1

Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway and are involved in a variety of developmental processes. Frizzled proteins each contain a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain, a variable-length linker region, a seven-pass transmembrane domain, and a variable-length C-terminal tail.

Entrez Gene IDs

8321 (Human); 14362 (Mouse)

Alternate Names

DKFZp564G072; FLJ95923; frizzled family receptor 1; frizzled homolog 1; frizzled, Drosophila, homolog of, 1; Frizzled1; Frizzled-1; fz-1; FZD1; fzE1; hFz1; seven transmembrane spanning receptor; Wnt receptor

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citation for Human/Mouse Frizzled-1 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1