Human/Mouse Frizzled-1 Antibody Summary
Gln72-His248 (Met122Ile)
Accession # O70421
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Frizzled-1 in HEK293 Human Cell Line transfected with Human Frizzled-1 by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with Frizzled-1 was stained with Rat Anti-Human/Mouse Frizzled-1 Monoclonal Antibody (Catalog # MAB11201, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram) followed by anti-Rat IgG APC-conjugated secondary antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.
View Larger
Detection of Frizzled-1 in HEK293 Human Cell Line by Flow Cytometry. HEK293 human embryonic kidney cell line was stained with Rat Anti-Human/Mouse Frizzled-1 Monoclonal Antibody (Catalog # MAB11201, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram) followed by anti-Rat IgG APC-conjugated secondary antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.
View Larger
Detection of Frizzled‑1 in NS0 cell line transfected with mouse Frizzled-1 by Flow Cytometry NS0 cell line transfected with mouse Frizzled-1 were stained with Rat Anti-Human/Mouse Frizzled‑1 Monoclonal Antibody (Catalog # MAB11201, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with saponin. View our protocol for Staining Intracellular Molecules.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Frizzled-1
The Wnt genes encode a large family of glycoproteins that are essential in development and tissue maintenance (1). Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway (2). Ten mouse and human Frizzled genes have been identified to date. The predicted structure of Frizzled proteins is similar among all family members, containing a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain (CRD), a variable-length linker region, a seven-pass transmembrane region, and a variable-length C-terminal cytoplasmic domain. The CRD comprises 642 amino acids and shares 95% identity with the human orthologue. Frizzled-1 mRNA has been detected in relatively large amounts in adult heart, placenta, lung, kidney, pancreas, prostate, and ovary, and in fetal lung and kidney (3). Several Frizzled-dependent signaling pathways exist (2). Their activation depends on the Wnt ligand and the cell context. Members of the low density lipoprotein receptor-related protein (LRP) are co-receptors for the Wnt ligands. LRP5/6 serve as co-receptors in the Wnt/Frizzled canonical pathway that alters gene expression via the stabilization of beta -catenin (4, 5). LRP1 may down regulate Wnt-3a/Frizzled-1 signaling in the canonical pathway by sequestering Frizzled-1 (6). Frizzled-1 is one of the purported Wnt-10b receptors whose signaling inhibits adipogenesis in preadipocytes (7). Frizzled-1 may be part of a feedback mechanism to modulate the effects of BMP-2 in mesenchymal cells since upregulation of its expression by BMP-2 counteracts the effects of BMP-2 and Wnt-3a in inducing the expression of the osteoblast differentiation marker, alkaline phosphatase (8).
- Wodarz, A. and R. Nusse (1998) Annu. Rev. Cell Dev. Biol. 14:59.
- Hsieh, J-C. (2004) Front. Biosci., 9:1333.
- Xu, L. et al. (2001) Matrix Biol. 2:147.
- Tamai, K. et al. (2000) Nature 407:530.
- Pinson, K. et al. (2000) Nature 407:535.
- Zielberberg, A. et al. (2004) J. Biol. Chem. 279:17535.
- Bennet, C. et al. (2002) J. Biol. Chem. 277:30998.
- Roman-Roman, S. et al. (2004) J. Biol. Chem. 279:5725.
Product Datasheets
Citations for Human/Mouse Frizzled-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
2
Citations: Showing 1 - 2
Filter your results:
Filter by:
-
Cross-talk between Wnt signaling pathways in human mesenchymal stem cells leads to functional antagonism during osteogenic differentiation.
Authors: Baksh D, Boland GM, Tuan RS
J. Cell. Biochem., 2007-08-01;101(5):1109-24.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
A regulatory circuit of miR-125b/miR-20b and Wnt signalling controls glioblastoma phenotypes through FZD6-modulated pathways
Authors: Tianzhi Huang, Angel A. Alvarez, Rajendra P. Pangeni, Craig M M. Horbinski, Songjian Lu, Sung-Hak Kim et al.
Nature Communications
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human/Mouse Frizzled-1 Antibody
There are currently no reviews for this product. Be the first to review Human/Mouse Frizzled-1 Antibody and earn rewards!
Have you used Human/Mouse Frizzled-1 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
